Plant Regeneration and Microprojectile-Mediated Gene Transfer in Embryonic Leaflets of Peanut (Arachis hypogaea L.)

Livingstone, D. Malcolm; Birch, RG

doi:10.1071/pp9950585

Cited by 24 publications

(23 citation statements)

References 28 publications

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“…The addition of kinetin to the GAs-containing medium further enhanced the number of elongated shoots. Livingstone and Birch (1995) observed substantial elongation of TDZ-derived shoots on 22.2 p.M BA medium, although the same concentration of BA did not bring about noticeable improvement in elongation of TDZ-derived peanut shoot primordia in our experiments. However, due to the inclusion of TDZ (1 gM) in the BA (22.2 l.tM)-supplemented medium, our results are not directly comparable with the previous report.…”

Section: Discussioncontrasting

confidence: 70%

Thidiazuron-induced highly morphogenic callus and high frequency regeneration of fertile peanut (Arachis hypogaea L.) plants

Gill

Ozias‐Akins

1999

In Vitro Cell.Dev.Biol.-Plant

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An efficient regeneration system was developed by culturing immature cotyledons and embryo axes ofArachis hypogaea L. cv. Georgia Green on Murashige and Skoog basal medium (MS) supplemented with various concentrations of thidiazuron (TDZ: 1, 5, 10, and 15 ~M). Highly morphogenic callus was produced from 100% of the explants comprising the cotyledon with attached embryo axis when cultured in the dark on 10 I, tM TDZ. Upon excision and continued culture in the dark on 10 I.tM TDZ, morphogenic callus grew repetitively during monthly subcultures and retained its regeneration potential. For organogenesis, a gradual reduction in TDZ concentration and exposure to light were necessary before transfer to MS basal medium. Inclusion of indole-3-butyric acid in liquid MS medium favored rooting of recovered shoots. A distinct feature of this investigation is the induction of highly morphogenic callus by TDZ and regeneration of morphologically normal, fertile peanut plants after 8 months of callus subculture.

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Section: Discussioncontrasting

confidence: 70%

Thidiazuron-induced highly morphogenic callus and high frequency regeneration of fertile peanut (Arachis hypogaea L.) plants

Gill

Ozias‐Akins

1999

In Vitro Cell.Dev.Biol.-Plant

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“…Using 4-d IILC plated on similar medium, they found the shoot fre quency of fastigiate lines Tamnut 74 and UPL Pn-4 to be higher than that of NC 7. Livingstone and Birch (1995) used MS basal medium + 1 mg L" 1 NAA + 3 mg L" 1 BAP and found that 50% of nonimbibed leaflets of cv. Robut (subsp.…”

Section: Resultsmentioning

confidence: 99%

High Efficiency Peanut Regeneration Using a Nonimbibed Immature Leaflet Culture Method

1996

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Efficient plant regeneration is an essential part of gene transfer systems using recombinant DNA technol ogy. Efficiency of regeneration from immature leaflets of peanut (Arachis hypogaea L.) was compared among several explant treatments in an effort to maximize recovery of plants from culture. In one experiment, explants were derived from leaflets of cv. NC 7 from dry mature seeds or from mature seeds which had been imbibed for 1 or 4 d. To avoid confounding treatment effects with variation among individual seeds, both nonimbibed and imbibed leaflets originated from a single seed. For each seed, four nonimbibed leaflets from a single leaf were excised, sterilized, rinsed, and plated on MS-based medium amended with 4 mg Lr 1 benzylaminopurine and 2 mg L' 1 naphthaleneacetic acid. The embryonic axis with the other leaf (four remaining leaflets) and one cotyledon attached was then imbibed in water for 1 or 4 d. After 4 wk in culture, 53% of nonimbibed leaflets, 37% of leaflets imbibed for 1 d, and 6% of leaflets imbibed for 4 d produced shoots. Subsequendy, regeneration efficiency was compared among immature leaflet cultures from nonimbibed seeds of four cultivars representing three botanical varieties from two subspecies. Shoot frequency after 4 wk in culture averaged 9% for Peruvian introduction A 2 (NC Ac 17090) representing subsp.fastigiata var. peruviana versus 53% for cv. NC 7 and Bolivian introduction B 2 (PI 274191), both representing subsp. hypogaea var. hypogaea. At 6 wk after plating, these frequencies increased to 28 and 61%, respectively. The response of Argentine introduc tion C 2 (PI 262000) representing subsp. fastigiata var. •Corresponding author.greater than in the other varieties, whereas it was signifi cantly lower in var. peruviana. Regenerated plants developed normal flowers and pods in the greenhouse. The study indicated that A. hypogaea can be cultured efficiently from nonimbibed leaflets.

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“…Particle gun bombardm ent initially develop ed by Sanford and his group at Cornell University (Sanford et al, 1987;Klein et al, 1987) has been successfully used for direct introduction of genes into a variety of plant species (see Varsha et al, 1997) including peanut. Embryogenic callus (Ozias-A kins et al, 1993;Livingstone and Birch, 1997), zygotic embryos (Schnall and Weissinger, 1993) and embryonic leaflets (Livingstone and Birch, 1995) have been used for transfo rmation using particle gun bombardment in peanut. Transient gene expression as assayed by GUS assay was affected by both particle size and amount of DNA used for coating and was found to be positively correlated with gene copy number (Lacorte et al, 1997).…”

Section: Dir Ect Dna Tr Ans Fer Usin G Particl E Gun Bomb Ardm Entmentioning

confidence: 99%

Regeneration and Genetic Transformation in Peanut: Current Status and Future Prospects

Eapen

2003

Focus on Biotechnology

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Peanut, Arachis hypogaea L. has long been the focus of conventional plant breeding efforts because of its importance as a source of high quality oil and protein. Recent techniques in genetic engineering coupled with developments in regeneration technology can be used for introduction of agronomically useful traits into established cultivars, which will supplement the conventional breeding programmes. Rapid strides have been made in the last two decades to develop a regeneration system for peanut. Highly reproducible regeneration systems through proliferation of axillary buds around a cultured rneristem, de novo shoot organogenesis and somatic embryogenesis are available today as target explants for experiments on transformation. Success has been achieved in the development of transgenic plants using both Agrobacterium-mediated and direct DNA transfer using particle gun bombardment. Several useful genes have already been transferred to peanut using gene transfer techniques . Peanut production is severely limited by a number of diseases and pests and one of the most challenging needs of the day is to improve resistance to Aspergillus species which produce aflatoxins -which are potent carcinogenic metabolites. In addition, introduction of value added traits such as altered protein/oil composition today lies within the realms of biotechnology. Developing peanut plants with genes for abiotic stress or edible vaccines is not far away. The candidate genes which are currently available for transfer to peanut with possible implications in groundnut breeding programme is discussed. As developments in plant biotechnology unfolds, high frequency, disease resistant, drought tolerant and good tasting peanut, which are safe to cat, will continue to be the goals of peanut biotechnologists .

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Plant Regeneration and Microprojectile-Mediated Gene Transfer in Embryonic Leaflets of Peanut (Arachis hypogaea L.)

Cited by 24 publications

References 28 publications

Thidiazuron-induced highly morphogenic callus and high frequency regeneration of fertile peanut (Arachis hypogaea L.) plants

Thidiazuron-induced highly morphogenic callus and high frequency regeneration of fertile peanut (Arachis hypogaea L.) plants

High Efficiency Peanut Regeneration Using a Nonimbibed Immature Leaflet Culture Method

Regeneration and Genetic Transformation in Peanut: Current Status and Future Prospects

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