Planar chromatography: Current status and future perspectives in pharmaceutical analysis (short review) — II. Special techniques and future perspectives in planar chromatography

Nyiredy, Szabolcs; Szepesi, G.

doi:10.1016/0731-7085(91)80113-n

Cited by 6 publications

(2 citation statements)

References 7 publications

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“…In AMD, several parameters must be considered to obtain the best separation: separation of solvents, eluent gradient, and number of steps [16,17].…”

Section: Separation By Amdmentioning

confidence: 99%

“…AMD is an instrumentation technique of planar chromatography [16,17] for the separation of compounds of similar chemical properties by using a solvent gradient on HPTLC plates. AMD presents the best resolution without any band diffusion and without oxidation because the microchamber is saturated with, for instance, methanol under a nitrogen atmosphere.…”

Section: Introductionmentioning

confidence: 99%

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Separation and quantification of terpenoids ofBoswellia serrata Roxb. extract by planar chromatography techniques (TLC and AMD)

Pozharitskaya¹,

Иванова²,

Shikov³

et al. 2006

J. Sep. Sci.

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An high-performance TLC (HPTLC) method for the separation of boswellic acids, the active constituents in Boswellia serrata extract, has been developed and TLC of these compounds on silica by automated multiple development (AMD) using solvent gradients was performed. Enhancement of the separation of boswellic acids on HPTLC plates was carried out by AMD chromatography. Densitometric analysis of the developed plate was carried out to quantify the four boswellic acids. 11-Keto-beta-boswellic acid (KBA) and acetyl-11-keto-beta-boswellic acid (AKBA) were quantified by densitometric scanning of the developed plate at 254 nm. beta-Boswellic acid (BA) and acetyl-beta-boswellic acid (ABA) were quantified after derivatization with anisaldehyde sulfuric acid reagent at 560 nm. The AMD system provided a clean separation according to polarity for each of the four groups studied and good results were obtained. The proposed HPTLC method for the simultaneous quantification of the major boswellic acids BA, ABA, KBA, and AKBA was found to be simple, precise, specific, sensitive, and accurate and can be used for routine quality control and for the quantification of these compounds in plant materials. The study of market products revealed significant variations in the content of these pharmacologically active compounds in commercial samples.

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“…In AMD, several parameters must be considered to obtain the best separation: separation of solvents, eluent gradient, and number of steps [16,17].…”

Section: Separation By Amdmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%