Separation and quantification of terpenoids ofBoswellia serrata Roxb. extract by planar chromatography techniques (TLC and AMD)

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A new procedure has been developed to separate and quantify the free radical-scavenging activity of individual compounds from an Emblica officinalis extract based on the combination of HPTLC with a diode array detector (DAD) and postchromatographic DPPH* radical derivatization. Free gallic and ellagic acids and emblicanins A and B in the E. officinalis extract were separated by TLC and identified. All the compounds of the extract were capable of scavenging of DPPH* radicals. It was established that the DPPH* scavenging activity of emblicanins A and B was 7.86 and 11.20 times more than that of ascorbic acid and 1.25 and 1.78 times more than gallic acid, respectively. From the estimated ID50 values, it can be seen that the increasing order of activity was emblicanin B > emblicanin A > gallic acid > ellagic acid > ascorbic acid. Probably, the antioxidant activity of E. officinalis extract is associated with the presence of hydrolyzable tannins having ascorbic acid-like action.

Section: Resultsmentioning

confidence: 99%

Separation and evaluation of free radical‐scavenging activity of phenol components of Emblica officinalis extract by using an HPTLC–DPPH^• method

Pozharitskaya¹,

Иванова²,

Shikov³

et al. 2007

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“…A HPTLC determination using a modern scanner with diode array detector (DAD) makes it possible to apply the extract containing solution directly on the HPTLC plates without prior preparation steps [12,13].…”

Section: Resultsmentioning

confidence: 99%

Separation and evaluation of free radical‐scavenging activity of phenol components of green, brown, and black leaves of Bergenia crassifolia by using HPTLC‐DPPH^• method

Pozharitskaya

Иванова²,

Shikov³

et al. 2007

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A new procedure has been developed to separate and quantify the free radical-scavenging activity of individual compounds from green, brown, and black leaves of Bergenia crassifolia based on the combination of high performance TLC (HPTLC) with a diode array detector (DAD) and postchromatographic 1,1-diphenyl-2-picrylhydrazyl radical (DPPH(*)) derivatization. Free gallic and ellagic acids, arbutin, hydroquinone, and bergenin in the B. crassifolia leaves' extracts were separated by HPTLC and identified. All compounds of the extract excluding bergenin were capable of scavenging DPPH * radicals. From the estimated ID(50) values, it can be seen that the increasing order of activity was gallic acid > arbutin > ellagic acid > hydroquinone > ascorbic acid. The antiradical activity of leaves of B. crassifolia is probably associated to the presence of phenol.

“…HPTLC determination using a modern scanner with DAD makes it possible to apply the extract containing solution directly on the HPTLC plates without prior preparation steps [11,12]. The optimum wavelength for quantification can easily be chosen via the DAD spectrum.…”

Section: Hptlc Assaymentioning

confidence: 99%

Comparison between HPLC and HPTLC densitometry for the determination of icariin from Epimedium koreanum extracts

Pozharitskaya¹,

Косман²,

Shikov³

et al. 2007

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Dry extracts of the aerial parts of Epimedium koreanum were quantified by HPLC and high performance TLC (HPTLC). A gradient HPLC method was used for the quantification of the prenylflavone glycoside icariin at 270 nm. A direct HPTLC assay was developed for the determination of icariin at 270 nm. The UV detection of both analytical assays were used to examine the purity of icariin peaks and compared with the standards. The assays provide good accuracy, reproducibility, and selectivity for the quantitative analysis of icariin. The icariin contents of five different dry extracts were compared by HPLC and HPTLC densitometry. The quantitative results of both analytical methods did not show any statistically significant differences between them, although a trend to slightly lower mean values could be found for the HPLC method.