High performance stationary phases for planar chromatography

Poole, Salwa K.; Poole, Colin F.

doi:10.1016/j.chroma.2010.10.072

Cited by 50 publications

(31 citation statements)

References 64 publications

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“…From Poole and Poole [38], the variance in the band, σ 2 chrom , was taken as: (6) where σ 2 obs is the variance of the separated (developed) analyte band, σ 2 SA is the variance of the band at its point of application, and σ 2 den is the variance of the densitometer used. Here, σ 2 den = 0 (no densitometer was used).…”

Section: Image and Data Analysismentioning

confidence: 99%

“…Efficiencies or numbers of theoretical plates were calculated from: (2) where W is the width of the analyte band. Observed plate heights and variances were calculated using an equation described by Poole and Poole [38]: (3) where Z o represents the distance between the solvent entry position and the position of the applied sample spot, and Z f represents the distance the solvent travels from its entry position. That is, Z sol = Z f − Z o , and Eq.…”

Section: Image and Data Analysismentioning

confidence: 99%

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Atomic layer deposition of aluminum-free silica onto patterned carbon nanotube forests in the preparation of microfabricated thin-layer chromatography plates

Kanyal¹,

Jensen²,

Dadson³

et al. 2014

Journal of Planar Chromatography – Modern TLC

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SummaryWe describe the direct, conformal, atomic layer deposition (ALD) of silica onto carbon nanotubes (CNTs) in the microfabrication of thinlayer chromatography (TLC) plates. As before, these plates were prepared with zig-zag hedge and channel microstructures, with high aspect ratio, porous hedges. After ALD, scanning electron microscopy (SEM) showed an increase in the radius of the CNTs of 8-40 nm. X-ray photoelectron spectroscopy (XPS) showed that the plates were composed almost entirely of silicon and oxygen, without contamination of metals or other elements that might compromise chromatographic performance, e.g., aluminum. Time-of-flight secondary ion mass spectrometry confirmed the extremely low level of aluminum in the plates. The final TLC layer thickness was ca. 50 μm. Separations of a test mixture of dyes from CAMAG (Muttenz, Switzerland) on an uncoated silica plate under traditional, normal phase conditions gave efficiencies of 40,000-140,000 plates m −1 with migration distances ranging from 2 to 36 mm. A separation of two fluorescent dyes, eosin Y disodium salt and sulforhodamine B, on an amino silane-coated plate gave efficiencies of ca. 170,000 and 200,000 plates m −1 , with hR F values of 76 and 88, respectively. Run times on these new plates were much faster than on conventional TLC plates.

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Section: Image and Data Analysismentioning

confidence: 99%

Section: Image and Data Analysismentioning

confidence: 99%

Atomic layer deposition of aluminum-free silica onto patterned carbon nanotube forests in the preparation of microfabricated thin-layer chromatography plates

Kanyal¹,

Jensen²,

Dadson³

et al. 2014

Journal of Planar Chromatography – Modern TLC

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“…[19]. Samples that require a high peak capacity for their separation and identification are usually better handled by HPLC.…”

Section: Attributes Of a Planar Formatmentioning

confidence: 99%

“…Under forced-flow conditions the minimum plate height is both higher and moved to a lower velocity compared to a fully optimized HPLC column, Figure 1.4 [14,19]. In addition, the optimum mobile phase FIGURE 1.4 Variation of the reduced plate height as a function of the reduced mobile phase velocity (Knox plot) for a high-performance silica gel layer using forced-flow development and comparison to an ideal column for HPLC.…”

Section: Consequences Of Capillary-controlled Flowmentioning

confidence: 99%

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Poole

2015

Instrumental Thin-Layer Chromatography

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Chromatography, Liquid

Poole¹

2013

Kirk-Othmer Encyclopedia of Chemical Technology

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Liquid chromatography is a separation technique with three main variants: column liquid chromatography, planar (thin‐layer) chromatography, and capillary electrochromatography. Separations are achieved by the distribution of sample components between a mobile liquid phase and (generally) a porous solid stationary phase. Selectivity differences result from the differences in adsorption, partition, size exclusion, molecular recognition, and electrostatic interactions in the two phases. Defined by mechanism, the main types of liquid chromatography are known as liquid–solid (normal‐phase), reversed‐phase, size‐exclusion, ion‐exchange, affinity, chiral, hydrophilic interaction, and hydrophobic interaction liquid chromatography. These separation mechanisms are supported by a wide range of stationary phase materials whose surface chemistry and morphology are designed for optimum performance selected from porous inorganic oxide, porous organic polymers, porous graphitic carbon, chemically bonded inorganic oxides, and chemically modified porous polymers. Liquid chromatographic methods can also be classified by speed, separation performance, and scale. Here, system properties defined by instrumental parameters have to be considered simultaneously with the physical properties of the stationary phase. Analytical and preparative‐scale applications require different instrument platforms. The method used to control the mobile phase flow (capillary forces, pneumatic forces, or electroosmosis) are further important to design criteria. Liquid chromatographic methods are also sustained by a variety of detection possibilities based on the principles of bulk property and solute property methods employing spectroscopic, electrochemical, light scattering, and ion formation techniques.

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High performance stationary phases for planar chromatography

Cited by 50 publications

References 64 publications

Atomic layer deposition of aluminum-free silica onto patterned carbon nanotube forests in the preparation of microfabricated thin-layer chromatography plates

Atomic layer deposition of aluminum-free silica onto patterned carbon nanotube forests in the preparation of microfabricated thin-layer chromatography plates

Milestones, Core Concepts, and Contrasts

Chromatography, Liquid

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