Pitch‐Dependent Acceleration of Neurite Outgrowth on Nanostructured Anodized Aluminum Oxide Substrates

Cho, Woo Kyung; Kang, Kyung‐Tae; Kang, Gyumin; Jang, Min Jee; Nam, Yoonkey; Choi, Insung S.

doi:10.1002/anie.201003307

Cited by 36 publications

(12 citation statements)

References 29 publications

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“…2). Similar responses were observed for osteoblastic [43], epithelial [44], and primary neuronal cell types [15] on various flat alumina substrates, indicating the importance of topography and potential of nanoporous AAM substrates as effective neural biomaterials.…”

Section: Resultssupporting

confidence: 65%

“…Several other cell types involving epithelial, muscle, blood, and neuronal origin have also been investigated against AAM to determine their adhesion, proliferation, and filopodia extension properties [5]. Studies examining the behavior of neuronal cells interfacing with AAM generally evaluate the influence of membrane topography and surface chemistry [14,15]. However, few studies have used AAM-modified silicon [16,17], complementary metal-oxide semiconductor [18], and gold electrode surfaces [19] to improve and control cell signal recording.…”

Section: Introductionmentioning

confidence: 99%

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Protein-releasing conductive anodized alumina membranes for nerve-interface materials

Altuntaş

Büyükserin

Haider

et al. 2016

Materials Science and Engineering: C

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Nanoporous anodized alumina membranes (AAMs) have numerous biomedical applications spanning from biosensors to controlled drug delivery and implant coatings. Although the use of AAM as an alternative bone implant surface has been successful, its potential as a neural implant coating remains unclear. Here, we introduce conductive and nerve growth factor-releasing AAM substrates that not only provide the native nanoporous morphology for cell adhesion, but also induce neural differentiation. We recently reported the fabrication of such conductive membranes by coating AAMs with a thin C layer. In this study, we investigated the influence of electrical stimulus, surface topography, and chemistry on cell adhesion, neurite extension, and density by using PC 12 pheochromocytoma cells in a custom-made glass microwell setup. The conductive AAMs showed enhanced neurite extension and generation with the electrical stimulus, but cell adhesion on these substrates was poorer compared to the naked AAMs. The latter nanoporous material presents chemical and topographical features for superior neuronal cell adhesion, but, more importantly, when loaded with nerve growth factor, it can provide neurite extension similar to an electrically stimulated CAAM counterpart.

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Section: Resultssupporting

confidence: 65%

Section: Introductionmentioning

confidence: 99%

Protein-releasing conductive anodized alumina membranes for nerve-interface materials

Altuntaş

Büyükserin

Haider

et al. 2016

Materials Science and Engineering: C

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“…[96] *Abbreviations: CVD: Chemical Vapor Deposition; DRG: Dorsal Root Ganglion; NGF: Nerve growth factor; PDMS: Polydimethylsiloxane; Si: Silicon Coating with N-(2-aminoethyl)-3aminopropyltri methoxysilane Effect of pitch: Neurite development was found to be much faster on surfaces with a 400 nm pitch than on surfaces with a 60 nm pitch. Major neurites stretched out earlier from the soma and grew more vigorously on the large-concave and nanoporous substrates [99] *Abbreviations: NGF: Nerve growth factor; R a : arithmetic average roughness parameter; R q : Root mean square roughness parameter; Si: Silicon 34 34…”

Section: Effect On Neuron Outgrowth and Neurite Outgrowth Orientationmentioning

confidence: 99%

“…Cho et al used a model of aluminium oxide concave nanostructures with a pitch of 60 (small)and 400 (large) nm, which were fabricated by electropolishing and electrochemical anodization. The large pitch was reported to exert an accelerating effect on hippocampal neuronal polarization or axon formation[99].…”

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confidence: 99%

Controlling the morphology and outgrowth of nerve and neuroglial cells: The effect of surface topography

2017

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There is increasing evidence that physical cues, such as topography, can have a significant impact on the neural cell functions. With the aid of micro-and nanofabrication techniques, new types of cell culture platforms are developed and the effect of surface topography on the cells has been studied. The present review article aims at reviewing the existing body of literature reporting on the use of various topographies to study and control the morphology and functions of cells from nervous tissue, i.e. the neuronal and the neuroglial cells. The cell responses-from phenomenology to investigation of the underlying mechanisms- on the different topographies, including both deterministic and random ones, are summarized.

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“…Since Choi et al. reported the accelerated neuronal development and neurite outgrowth of hippocampal neurons cultured on the nanostructured anodized aluminum oxide (AAO) substrate with 400‐nm pitch (Cho et al., 2010), the developmental behaviors of neurons, especially neurites, have intensively been investigated on various nanotopographies. In particular, nanotopographic effects and their influential scope on neurons were investigated for the early period of neuronal development when the neurites begin to sprout and grow.…”

Section: Directional Movement Of Neurons On Physical Cuesmentioning

confidence: 99%

Neuro‐taxis: Neuronal movement in gradients of chemical and physical environments

Seo

Youn

Choi

et al. 2020

Developmental Neurobiology

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Environmental chemical and physical cues dynamically interact with migrating neurons and sprouting axons, and in particular, the gradients of environmental cues are regarded as one of the factors intimately involved in the neuronal movement. Since a growth cone was first described by Cajal, more than one century ago, chemical gradients have been suggested as one of the mechanisms by which the neurons determine proper paths and destinations. However, the gradients of physical cues, such as stiffness and topography, which also interact constantly with the neurons and their axons as a component of the extracellular environments, have rarely been noted regarding the guidance of neurons, despite their gradually increasingly reported influences in the case of nonneuronal‐cell migration. In this review, we discuss chemical (i.e., chemo‐ and hapto‐) and physical (i.e., duro‐) taxis phenomena on the movement of neurons including axonal elongation. In addition, we suggest topotaxis, the most recently proposed physical‐taxis phenomenon, as another potential mechanism in the neuronal movement, based on the reports of neuronal recognition of and responses to nanotopography.

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Pitch‐Dependent Acceleration of Neurite Outgrowth on Nanostructured Anodized Aluminum Oxide Substrates

Cited by 36 publications

References 29 publications

Protein-releasing conductive anodized alumina membranes for nerve-interface materials

Protein-releasing conductive anodized alumina membranes for nerve-interface materials

Controlling the morphology and outgrowth of nerve and neuroglial cells: The effect of surface topography

Neuro‐taxis: Neuronal movement in gradients of chemical and physical environments

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