Controlling the morphology and outgrowth of nerve and neuroglial cells: The effect of surface topography

Simitzi, Chara; Ranella, Anthi; Stratakis, Emmanuel

doi:10.1016/j.actbio.2017.01.023

Cited by 184 publications

(177 citation statements)

References 127 publications

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“…Several investigations have evaluated the influence of topographic cues on neurite growth. The majority of these studies used microgrooves with depths ranging from 200 nm to 69 μm 57 . While the grooves used in previous studies usually have well-defined shapes, i.e., square cross section with sharp edges, the aligned polymer structures on our β-PVDF films are much less ordered and exhibit small heights (<50 nm, Supplementary Fig.…”

Section: Resultsmentioning

confidence: 99%

Ultrasound-mediated piezoelectric differentiation of neuron-like PC12 cells on PVDF membranes

Hoop

Chen

Ferrari

et al. 2017

Sci Rep

149

134

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Electrical and/or electromechanical stimulation has been shown to play a significant role in regenerating various functionalities in soft tissues, such as tendons, muscles, and nerves. In this work, we investigate the piezoelectric polymer polyvinylidene fluoride (PVDF) as a potential substrate for wireless neuronal differentiation. Piezoelectric PVDF enables generation of electrical charges on its surface upon acoustic stimulation, inducing neuritogenesis of PC12 cells. We demonstrate that the effect of pure piezoelectric stimulation on neurite generation in PC12 cells is comparable to the ones induced by neuronal growth factor (NGF). In inhibitor experiments, our results indicate that dynamic stimulation of PVDF by ultrasonic (US) waves activates calcium channels, thus inducing the generation of neurites via a cyclic adenosine monophosphate (cAMP)-dependent pathway. This mechanism is independent from the well-studied NGF induced mitogen-activated protein kinases/extracellular signal-regulated kinases (MAPK/ERK) pathway. The use of US, in combination with piezoelectric polymers, is advantageous since focused power transmission can occur deep into biological tissues, which holds great promise for the development of non-invasive neuroregenerative devices.

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Section: Resultsmentioning

confidence: 99%

Ultrasound-mediated piezoelectric differentiation of neuron-like PC12 cells on PVDF membranes

Hoop

Chen

Ferrari

et al. 2017

Sci Rep

149

134

View full text Add to dashboard Cite

show abstract

“…Contact guidance refers to the phenomenon where adherent cells react to material surfaces with different surface topographies . As the cytoskeleton plays a major role in cellular fate, it is important to determine the morphology of adherent cells on scaffolds . Our results revealed that the general morphologies of cardiomyoblasts were controlled by the laser‐ablated microscale groove direction on the hierarchically patterned fibrous scaffolds.…”

Section: Discussionmentioning

confidence: 99%

Effect of spatial arrangement and structure of hierarchically patterned fibrous scaffolds generated by a femtosecond laser on cardiomyoblast behavior

Jun

Kim

Chung

et al. 2018

J Biomedical Materials Res

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Biological responses on biomaterials occur either on their surface or at the interface. Therefore, surface characterization is an essential step in the fabrication of ideal biomaterials for achieving effective control of the interaction between the material surface and the biological environment. Herein, we applied femtosecond laser ablation on electrospun fibrous scaffolds to fabricate various hierarchical patterns with a focus on the alignment of cells. We investigated the simultaneously stimulated response of cardiomyoblasts based on multiple topographical cues, including scales, oriented directions, and spatial arrangements, in the fibrous scaffolds. Our results demonstrated a synergistic effect on cell behaviors of one or more structural arrangements in a homogeneous orientation, whereas antagonistic effects were observed for cells arranged on a surface with heterogeneous directions. Taken together, these results indicate that our hierarchically patterned fibrous scaffolds may be useful tools for understanding the cellular behavior on fibrous scaffolds used to mimic an extracellular matrix-like environment. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1732-1742, 2018.

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“…The stem-cell responses, including adhesion, morphology, proliferation, and differentiation to different continuous, discontinuous, andr andom roughness topographies are summarized in Ta bles 1, 2and 3, respectively.The classification of the topographies into continuous, discontinuousa nd random was introduced in our previous review paper. [50] Briefly,p rimaryc lassification categorizes the various deterministic pattern geometries into continuous and discontinuous. As econd classification divides further the continuous geometries into anisotropic and isotropic topographies.…”

Section: The Effect Of Topographical Cues On Neural Stem Cellsmentioning

confidence: 99%

Controlling the Outgrowth and Functions of Neural Stem Cells: The Effect of Surface Topography

et al. 2018

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Neural stem cells (NSCs) are self-renewing cells that generate the major cell types of the central nervous system, namely neurons, astrocytes and oligodendrocytes, during embryonic development and in the adult brain. NSCs reside in a complex niche where they are exposed to a plethora of signals, including both soluble and physical signals such as compressive and shear stresses, but also discontinuities and differences in morphology of the extracellular environment, termed as topographical features. Different approaches that incorporate artificial micro- and nano-scale surface topographical features have been developed aiming to recapitulate the in vivo NSC niche discontinuities and features, particularly for in vitro studies. The present review article aims at reviewing the existing body of literature on the use of artificial micro- and nano-topographical features to control NSCs orientation and differentiation into neuronal and/or neuroglial lineage. The different approaches on the study of the underlying mechanism of the topography-guided NSC responses are additionally revised and discussed.

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Controlling the morphology and outgrowth of nerve and neuroglial cells: The effect of surface topography

Cited by 184 publications

References 127 publications

Ultrasound-mediated piezoelectric differentiation of neuron-like PC12 cells on PVDF membranes

Ultrasound-mediated piezoelectric differentiation of neuron-like PC12 cells on PVDF membranes

Effect of spatial arrangement and structure of hierarchically patterned fibrous scaffolds generated by a femtosecond laser on cardiomyoblast behavior

Controlling the Outgrowth and Functions of Neural Stem Cells: The Effect of Surface Topography

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