Piperlongumine potentiates the antitumor efficacy of oxaliplatin through ROS induction in gastric cancer cells

Zhang, Peichen; Shi, Lingyan; Zhang, Tingting; Lin, Hong; He, Wei; Cao, Peihai; Shen, Xin; Zheng, Peisen; Xia, Yiqun; Zou, Peng

doi:10.1007/s13402-019-00471-x

Cited by 38 publications

(19 citation statements)

References 44 publications

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“…Previous research have indicated that the anti-tumor effect of oxaliplatin is closely related to the ROS accumulation. 40,41 ASK1 is a serine/threonine protein kinase that acts as downstream molecule of ROS. 42 As a key intermediate-bridge of ROSdependent apoptotic pathway, activated ASK1 induces apoptotic cell death through phosphorylating the JNK which is one member of MAPK family.…”

Section: Discussionmentioning

confidence: 99%

<p>MiR-138 Suppresses the PDK1 Expression to Decrease the Oxaliplatin Resistance of Colorectal Cancer</p>

Wang¹,

Zhang²,

Yao³

et al. 2020

OTT

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Background: Oxaliplatin is one kind of platinum-based drug. It is effective and commonly used in the treatment of colorectal cancer (CRC). However, development of acquired drug resistance is still a big obstacle during the oxaliplatin therapy. It is urgent to take strategies to decrease the oxaliplatin resistance of CRC. Materials and Methods: Oxaliplatin-resistant HT29 and SW480 (HT29/R and SW480/R) cells were acquired through long-term exposure to oxaliplatin by using the routine HT29 and SW480 cells. Relative glucose consumption, lactate generation and LDH activity were tested to evaluate the glycolysis of CRC cell lines. MTT assays were conducted to evaluate the differences of oxaliplatin sensitivity between HT29/R (SW480/R) cells and their parental HT29 (SW480) cells. Regulation of miR-138 on PDK1 was confirmed through qRT-PCR, Western blot and dual-luciferase reporter assays. Reactive oxygen species (ROS) levels were measured by flow cytometry. Results: HT29/R and SW480/R cells exhibited higher glucose consumption, lactate production and LDH activity compared to their parental HT29 and SW480 cells. However, oxygen consumption rate (OCR) in HT29/R and SW480/R cells is lower than that in HT29 and SW480 cells, respectively. Results of MTT assays showed that treatment with miR-138 can increase the cytotoxicity of oxaliplatin to HT29/R and SW480/R cells. Research on mechanisms showed that PDK1 was the target of miR-138. Overexpression of miR-138 can inhibit the expression of PDK1, and thus increase the OCR of HT29/R and SW480/R cells. Under the treatment of oxaliplatin, the miR-138-overexpressed HT29/R and SW480/R cells generated more amount of ROS to get into the apoptosis process. Conclusion: Overexpression of miR-138 suppressed the PDK1 expression to decrease the oxaliplatin resistance of CRC.

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Section: Discussionmentioning

confidence: 99%

<p>MiR-138 Suppresses the PDK1 Expression to Decrease the Oxaliplatin Resistance of Colorectal Cancer</p>

Wang¹,

Zhang²,

Yao³

et al. 2020

OTT

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“…[8] PL effectively inhibit cancer cell proliferation via increased ROS-mediated apoptosis, thereby it have anti-tumor property. [9,10,11] PL is a novel compound, it increases the biological activity through structural changes during neuronal protection. [12] PL has been reported to control cell proliferation in cancer cells, inhibits ROS production, and apoptosis induction in H 2 O 2 and 6hydroxydopamine-induced neuron cells and PC12 cells.…”

mentioning

confidence: 99%

Piperlongumine attenuates oxidative stress, inflammatory, and apoptosis through modulating the GLUT‐2/4 and AKT signaling pathway in streptozotocin‐induced diabetic rats

Xiao

Chi

2021

J Biochem & Molecular Tox

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The current study was done to measure the role of piperlongumine (PL) on hyperglycemia interrelated oxidative stress-mediated inflammation and apoptosis, inflammatory stress, and the diabetic insulin receptor substrate 2 (IRS2), protein kinase B (AKT), and glucose transporter 2 (GLUT-2)/4 signaling pathway in streptozotocin (STZ)-persuaded diabetic animals. Diabetes was initiated in experimental animals via a single dose intraperitoneal inoculation of STZ. Diabetic rats revealed an augmented blood-glucose level with drastically diminished plasma-insulin status.The functions of antioxidants were diminished with enhanced lipid peroxidation, conjugated dienes, and protein carbonyls noticed in diabetic rats' plasma and pancreatic tissues. An elevation of nuclear factor-κB (NF-κB), tumor necrosis factorα, and interleukin-6 proteins was noticed in pancreatic tissues as well as IRS2, AKT, GLUT-2, and GLUT-4 marker expressions were quantified in the hepatic tissue of control and diabetic rats. Oral administration of PL for 30 days drastically lowered glucose and higher insulin status in STZ-induced diabetic rats. Impressively, PL oral supplementation considerably restored the antioxidant levels and reduced inflammation and diabetic marker expressions in STZ-diabetic rats. These results were supported through a histological study. Moreover, PL also augmented the level of B-cell lymphoma 2 and diminished the level of Bcl-2-associated X protein in STZ-treated rat's hepatic tissues. Thus, we concluded that PL excellently rescued pancreatic β cells through mitigating hyperglycemia via dynamic insulin secretion, activating antioxidants, and inhibiting inflammation and apoptosis in the pancreatic and hepatic tissue of diabetic rats.

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“…Piperlongumine has been found to be effective against several cancers such as multiple myeloma [124], melanoma [125], pancreatic cancer [126], colon cancer [127,128] oral squamous cell carcinoma [129], non-small-cell lung cancer [130], gastric cancer [131], biliary cancer [132], and prostate cancer [133]. The mechanism of the anti-cancer effects of piperlongumine is listed in Table 1.…”

Section: Piperlonguminementioning

confidence: 99%

Role of Phytochemicals in Cancer Prevention

Ranjan

Ramachandran

Gupta

et al. 2019

IJMS

228

111

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The use of synthetic, natural, or biological agents to minimize the occurrence of cancer in healthy individuals is defined as cancer chemoprevention. Chemopreventive agents inhibit the development of cancer either by impeding DNA damage, which leads to malignancy or by reversing or blocking the division of premalignant cells with DNA damage. The benefit of this approach has been demonstrated in clinical trials of breast, prostate, and colon cancer. The continuous increase in cancer cases, failure of conventional chemotherapies to control cancer, and excessive toxicity of chemotherapies clearly demand an alternative approach. The first trial to show benefit of chemoprevention was undertaken in breast cancer patients with the use of tamoxifen, which demonstrated a significant decrease in invasive breast cancer. The success of using chemopreventive agents for protecting the high risk populations from cancer indicates that the strategy is rational and promising. Dietary components such as capsaicin, cucurbitacin B, isoflavones, catechins, lycopenes, benzyl isothiocyanate, phenethyl isothiocyanate, and piperlongumine have demonstrated inhibitory effects on cancer cells indicating that they may serve as chemopreventive agents. In this review, we have addressed the mechanism of chemopreventive and anticancer effects of several natural agents.

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Piperlongumine potentiates the antitumor efficacy of oxaliplatin through ROS induction in gastric cancer cells

Cited by 38 publications

References 44 publications

<p>MiR-138 Suppresses the PDK1 Expression to Decrease the Oxaliplatin Resistance of Colorectal Cancer</p>

<p>MiR-138 Suppresses the PDK1 Expression to Decrease the Oxaliplatin Resistance of Colorectal Cancer</p>

Piperlongumine attenuates oxidative stress, inflammatory, and apoptosis through modulating the GLUT‐2/4 and AKT signaling pathway in streptozotocin‐induced diabetic rats

Role of Phytochemicals in Cancer Prevention

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