Pilot Study on the Genetic Background of an Active Matrix Metalloproteinase‐8 Test in Finnish Adolescents

Heikkinen, Anna Maria; Raivisto, Teija; Kettunen, K; Kovanen, Leena; Haukka, Jari; Esmaeili, Elmira Pakbaznejad; Elg, Jessica; Gieselmann, Dirk-Rolf; Rathnayake, Nilminie; Ruokonen, Hellevi; Tervahartiala, Taina; Sorsa, Timo

doi:10.1902/jop.2016.160441

Cited by 35 publications

(66 citation statements)

References 28 publications

(54 reference statements)

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“…Additionally, it identifies genetically predisposed adolescents [73]. A positive aMMP-8 test is based on a cutoff of 25 ng aMMMp-8 per milliliter of filtrate derived from 5 mL mouth rinse.…”

Section: Development Of Point Of Care/chairside Test For Oral (Permentioning

confidence: 99%

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Salivary Diagnostics—Point-of-Care diagnostics of MMP-8 in dentistry and medicine

et al. 2017

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Human saliva is an easily accessible biological fluid and contains a variety of disease-related biomarkers, which makes it a potential diagnostic medium. The clinical use of salivary/oral fluid biomarkers to identify oral and systemic conditions requires the development of non-invasive screening and diagnostic technologies, and is among the main goals of oral fluid researchers. The analysis of the disease-specific oral and systemic biomarkers in saliva and oral fluids (i.e., mouth-rinse, gingival crevicular fluid (GCF) and peri-implantitis sulcular fluid (PISF)) is demanding. Several factors influence their expression and release; these factors include the intracellular location, the molecular size and the flow characteristics of the biological fluid. The type of saliva/oral fluid utilized for the diagnostics affects the analysis. High sensitivity together with sophisticated methods and techniques are essential to get a useful outcome. We describe here a recently developed mouth-rinse that is practical, convenient and inexpensive, as well as PISF chair-side/point of care (PoC) lateral-flow active matrix metalloproteinase (aMMP-8) immunoassays to detect, predict and monitor the course and treatment of periodontitis and peri-implantitis.

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Section: Development Of Point Of Care/chairside Test For Oral (Permentioning

confidence: 99%

“…The test has been independently and internationally successful validated in Africa, Europe and USA, and in all studies it found to excellently differentiate periodontal health and disease [71,73,74,75,76]. …”

Section: Development Of Point Of Care/chairside Test For Oral (Permentioning

confidence: 99%

Salivary Diagnostics—Point-of-Care diagnostics of MMP-8 in dentistry and medicine

et al. 2017

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“…Indeed, a quantitative POC activated MMP8 (aMMP8) oral fluid test has been repeatedly and independently validated in Finland, Germany, Nigeria, Turkey, the Netherlands and the United States to successfully screen susceptible sites and patients, differentiate active and inactive perio dontal sites, predict the future disease progression and monitor treatment response and mainten ance therapy [3][4][5][6][7][8] , with a diagnostic sensitivity and specificity of 76-83% and 96%, respectively, and a turnaround time of 5-7 minutes. Furthermore, the test can identify initial periodontitis in genetically predisposed adolescents 5 .…”

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confidence: 99%

“…Furthermore, the test can identify initial periodontitis in genetically predisposed adolescents 5 . Thus, the aMMP8 test is effective in both adolescent and adult populations 3,4 .…”

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confidence: 99%

“…The test is, therefore, very suitable for monitoring disease progression and tailor ing preventive and therapeutic measures 14 . The aMMP8 test is inexpensive, easy to use (the results are automated and, therefore, independent of the practitioner's experience) and currently available for routine use by dental and medical professionals linking these disciplines [3][4][5]8,15 . Finally, we agree with the authors of the Primer that additional work, especially on the prognostic value of bio markers in periodontal and peri-implant diseases, is still required.…”

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confidence: 99%

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A quantitative point-of-care test for periodontal and dental peri-implant diseases

Sorsa

Gieselmann

Arweiler

et al. 2017

Nat Rev Dis Primers

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142

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Implementing of aMMP‐8 point‐of‐care test with a modified new disease classification in Finnish adolescent cohorts

Heikkinen

Raivisto²,

Räisänen

et al. 2022

Clinical & Exp Dental Res

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Objectives: Periodontitis is a multifactorial biofilm-induced inflammatory disease; however, clinical and radiographic information reflects events that have already occurred. aMMP-8 lateral-flow chairside or point-of-care-test (POC-test) results have also come to complement the overall status of the patient's current situation. The current study aimed to clarify the usefulness of aMMP-8 chairsidetest (aMMP-8 POCT) results to alert the initial or early periodontitis in adolescents, a potential periodontitis risk group with cut off 20 ng/ml in Stage I periodontitis, according to the current periodontitis classification system.Material and Methods: A total of 117 adolescents were aMMP-8 POC tested for aMMP-8 levels and examined for the full mouth and were interviewed for questions concerning health behavior.Results: Of all 117 participants, N = 38 (32.5%) was aMMP-8 POCT positive, and N = 34 (29.1%) had at least one 4 mm periodontal deepened pocket; however, only N = 16 (13.7%) had both characteristics. The subclinical stage (N = 47) could not be classified either as gingivitis or Stage I. Of the participants, who did not have any deepened periodontal pockets, 18 tested negative.Conclusions: Stage I is preceded by antecedent stages that should be tackled by oral healthcare prevention and personalized treatment modalities by professionals. Elevated (>20 ng/ml), positive aMMP-8 POCT results can be regarded as initial alarmer such as emerging risk. This should be utilized in the preventive personalized interventions by oral health professionals.

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Pilot Study on the Genetic Background of an Active Matrix Metalloproteinase‐8 Test in Finnish Adolescents

Abstract: Genetic polymorphisms of MMP3 and VDR are linked to initial periodontitis in Finnish adolescents, and the aMMP-8 chairside test can eventually detect initial periodontitis in young patients with predisposing genetic background.

Cited by 35 publications

References 28 publications

Salivary Diagnostics—Point-of-Care diagnostics of MMP-8 in dentistry and medicine

Salivary Diagnostics—Point-of-Care diagnostics of MMP-8 in dentistry and medicine

A quantitative point-of-care test for periodontal and dental peri-implant diseases

Implementing of aMMP‐8 point‐of‐care test with a modified new disease classification in Finnish adolescent cohorts

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