A quantitative point-of-care test for periodontal and dental peri-implant diseases

Sorsa, Timo; Gieselmann, Dirk; Arweiler, Nicole Birgit; Hernández, Marcela

doi:10.1038/nrdp.2017.69

Cited by 74 publications

(151 citation statements)

References 15 publications

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“…It offers an inexpensive, quick and easy way to predictively diagnose and screen adolescents with early signs of subclinical periodontitis and with predisposing genetic background, even before radiological findings . Currently, the chairside/point‐of‐care aMMP‐8 test can be performed quantitatively . Combining the aMMP‐8 chairside test result with other patient‐related risk factor information, e.g., sex, smoking status, and clinical periodontal examination, could really aid oral health professionals in identification of subclinical periodontitis patients early enough.…”

Section: Discussionmentioning

confidence: 99%

Cross‐sectional analysis of risk factors for subclinical periodontitis; active matrix metalloproteinase‐8 as a potential indicator in initial periodontitis in adolescents

Heikkinen

Räisänen

Tervahartiala

et al. 2018

Journal of Periodontology

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Background The aim of this study was to investigate how different patient‐related risk indicators might be associated with the odds of developing subclinical periodontitis in adolescents. Methods This cross‐sectional study included 252 Finnish individuals aged 15 to 16 years, of whom 141 were boys and 111 girls. A specially trained dentist performed clinical examinations: measurements included periodontal indexes (bleeding on probing, visible plaque index, root calculus, and probing depth, smoking by pack‐years, periodontal bacteria (Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia, Prevotella intermedia, Prevotella nigrescens, and Treponema denticola) and the potential salivary periodontal biomarkers (active matrix metalloproteinase‐8 [aMMP‐8], polymorphonuclear leukocyte elastase [PMN elastase], and total protein, albumin, immunoglobulin A, immunoglobulin G, and immunoglobulin M). Results were analyzed by ordinal logistic regression, one‐way analysis of variance, Fisher exact test, and Kruskal‒Wallis H test. Results The main finding of this study was that subclinical periodontitis in adolescents was statistically significantly associated with elevated salivary aMMP‐8 but not with PMN elastase. Also, adolescents with subclinical periodontitis had statistically significantly higher levels of bleeding on probing, root calculus, and dental plaque than adolescents without subclinical periodontitis. Conclusions We suggest that the main risk factor for subclinical periodontitis in adolescents is the partly calcified, dysbiotic bacterial biofilm, which interacts with the immune defenses of the host; this leads to gingival inflammation and eventually to deepening periodontal pockets. This proinflammatory subclinical periodontitis stage, which represents stage I periodontitis in the new classification, is reflected as elevated salivary aMMP‐8 levels in oral fluids.

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Section: Discussionmentioning

confidence: 99%

Cross‐sectional analysis of risk factors for subclinical periodontitis; active matrix metalloproteinase‐8 as a potential indicator in initial periodontitis in adolescents

Heikkinen

Räisänen

Tervahartiala

et al. 2018

Journal of Periodontology

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“…22,23 Activated matrix metalloproteinase-8 (aMMP-8) is already known as one of the most validated periodontitis biomarkers. 24,29,30,[36][37][38][39][40][41][42][43][44][45][46] Moreover, previously there have been found a moderate positive association/correlation between MMP-8/PGLYRP1 axis and TREM-1 levels among adults, 14 but to the best of authors' knowl-edge, this association hasn't been studied among adolescent subjects before. [24][25][26][27][28][29][30][31][32][33][34][35] This stage can be measured using a point-of-care (PoC) lateral flow collagenase-2 (aMMP-8) immunotest.…”

Section: Introductionmentioning

confidence: 99%

A point‐of‐care test of active matrix metalloproteinase‐8 predicts triggering receptor expressed on myeloid cells‐1 (TREM‐1) levels in saliva

Räisänen

Heikkinen

Esmaeili

et al. 2019

Journal of Periodontology

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Background This cross‐sectional study aims to investigate if a point‐of‐care (PoC) test of active matrix metalloproteinase‐8 (aMMP‐8) predicts levels of inflammation amplifier triggering receptor expressed on myeloid cells‐1 (TREM‐1) and its putative ligand the neutrophil peptidoglycan recognition protein 1 (PGLYRP1) in saliva. Methods Forty‐seven adolescents, aged 15 to 17 years, were tested with aMMP‐8 PoC test, which was followed by a full‐mouth clinical examination of the assessment of periodontal, mucosal, and oral health. TREM‐1 and PGLYRP1 levels were analyzed by ELISA. The immunofluorometric assay (IFMA) specific for aMMP‐8 was used as the reference method. Results Fourteen saliva samples out of a total of 47 showed positivity for aMMP‐8 PoC test. Both the TREM‐1 and the aMMP‐8 (IFMA) levels were significantly elevated among the aMMP‐8 PoC test positives compared with the PoC test negatives (P < 0.05). Moreover, aMMP‐8 levels assessed by IFMA showed a strong positive correlation with TREM‐1 levels in saliva (r = 0.777, P < 0.001). The number of sites with a probing depth of ≥4 mm was significantly lower among the adolescents that had a negative aMMP‐8 PoC test result, and TREM‐1 levels < 75 pg/mL (P < 0.05). In contrast, adolescents with a positive aMMP‐8 PoC test result (i.e., elevated aMMP‐8 levels) together with elevated TREM‐1 levels had a significantly higher number of periodontal pockets with ≥4 mm (P < 0.001). Conclusion The present study validated usability of aMMP‐8 PoC test for predicting “proinflammatory” salivary profile and periodontal health status in adolescents.

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“…For instance, salivary interleukin (IL)‐1beta (IL‐1β), MMP‐8, osteoprotegerin, macrophage inflammatory protein‐1α, and prostaglandin E2 have been reported to reflect the response to periodontal therapy and are suggested to be useful for monitoring periodontal disease status, and the combination of MMP‐8 with IL‐1β and Porphyromonas gingivali s can be a successful tool for diagnosing periodontitis . Based on the results of these studies, MMP‐8‐based point‐of‐care diagnostic kits were developed to screen and monitor periodontal and peri‐implant health . In addition, the reduction in activity of alanine aminotransferase, P. gingivalis , soluble toll‐like receptor‐2, lactoferrin, and arginine have been reported to be associated with clinical improvement following nonsurgical periodontal therapy .…”

Section: Introductionmentioning

confidence: 99%

The potential of salivary biomarkers for predicting the sensitivity and monitoring the response to nonsurgical periodontal therapy: A preliminary assessment

Lee

Chen

et al. 2018

J of Periodontal Research

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A quantitative point-of-care test for periodontal and dental peri-implant diseases

Cited by 74 publications

References 15 publications

Cross‐sectional analysis of risk factors for subclinical periodontitis; active matrix metalloproteinase‐8 as a potential indicator in initial periodontitis in adolescents

Cross‐sectional analysis of risk factors for subclinical periodontitis; active matrix metalloproteinase‐8 as a potential indicator in initial periodontitis in adolescents

A point‐of‐care test of active matrix metalloproteinase‐8 predicts triggering receptor expressed on myeloid cells‐1 (TREM‐1) levels in saliva

The potential of salivary biomarkers for predicting the sensitivity and monitoring the response to nonsurgical periodontal therapy: A preliminary assessment

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