Pigmentation of the enamel of albino rat incisor teeth

Stein, Gisele Guiomara; Boyle, P.E.

doi:10.1016/0003-9969(59)90002-0

Cited by 29 publications

(8 citation statements)

References 6 publications

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“…Early studies also led to two main and seemingly contradictory ideas regarding the function of iron in enamel. Stein and Boyle [27] have concluded that pigmentation does not affect enamel structural properties based on the observation that after surgically destroying the pigment-containing part of the enamel organ, the integrity of the underlying enamel is not affected. On the other hand, Prime et al [28] showed that prolonged iron deficiency caused loss of pigmentation as well as enamel hypoplasia and aplasia, suggesting iron deficiency is associated with severe structural defects of enamel.…”

Section: Discussionmentioning

confidence: 99%

Iron Deposition and Ferritin Heavy Chain (Fth) Localization in Rodent Teeth

2013

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BackgroundAn iron rich layer on the labial surface is characteristic of the enamel of rodent incisors. In order to address a role for iron content in continuously growing incisors during odontogenesis, we studied iron deposition patterns in enamel and dentine using Perls’ blue staining and ferritin heavy chain (Fth) immunolocalization. Fth expression is regulated by iron level; therefore its localization can be used as a sensitive indicator for iron deposition.ResultsSagittal sections of 4-week old rat incisors showed a gradual increase in iron level in the enamel organ from secretory to maturation stages. In addition, iron was detected in ameloblasts of erupting third molars of 4-week old rats, suggesting iron plays a role in both incisor and molar development. In odontoblasts, the presence of iron was demonstrated, and this is consistent with iron’s role in collagen synthesis. Using postnatal 3-, 6-, 9-day old mice, the spatial and temporal expression of Fth in tooth development again indicated the presence of iron in mature ameloblasts and odontoblasts.ConclusionsWhile these data do not explain what functional role iron has in tooth formation, it does highlight a significant molecular activity associated with the formation of the rodent dentition.

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Section: Discussionmentioning

confidence: 99%

Iron Deposition and Ferritin Heavy Chain (Fth) Localization in Rodent Teeth

2013

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“…Studying the rat incisor under a light microscope, STEIN and BOYLE (1959) observed that the enamel cuticle, a colorless and structureless membrane, appeared at the portion where the full thickness of enamel was established and that it persisted unchanged to the incisal edge. STERN (1962) was the first to study the DEJ of the rat incisor under an electron microscope and clearly observed a membranous structure he called a cuticular membrane.…”

Section: Discussionmentioning

confidence: 99%

Cytochemical Studies of Ameloblasts and the Surface Layer of Enamel of the Rat Incisor at the Maturation Stage

Takano

1979

Arch. Histol. Jap., Arch. Histol. Jpn

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“…Among them, the iron utilization in the rodent enamel organ illustrates one of the most interesting examples of iron usage in mammals. Iron deposited on to the enamel surface seems to contribute to the formation of acid resistance and hardness of the rodent incisors, which is advantageous for grinding the hard seeds in the environment (Halse 1974; Stein & Boyle 1959). In fact, the diminished acid resistance of iron‐poor Nrf2 −/– teeth (Fig.…”

Section: Discussionmentioning

confidence: 99%

Nrf2 deficiency causes tooth decolourization due to iron transport disorder in enamel organ

Yanagawa¹,

Itoh

Uwayama³

et al. 2004

Genes to Cells

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Rodents have brownish-yellow incisors whose colour represents their iron content. Iron is deposited into the mature enamel by ameloblasts that outline enamel surface of the teeth. Nrf 2 is a basic regionleucine zipper type transcription factor that regulates expression of a range of cytoprotective genes in response to oxidative and xenobiotic stresses. We found that genetically engineered Nrf 2-deficient mice show decolourization of the incisors. While incisors of wild-type mice were brownish yellow, incisors of Nrf 2-deficient mice were greyish white in colour. Micro X-ray imaging analysis revealed that the iron content in Nrf 2-deficient mouse incisors were significantly decreased compared to that of wild-type mice. We found that iron was aberrantly deposited in the papillary layer cells of enamel organ in Nrf 2-deficient mouse, suggesting that the iron transport from blood vessels to ameloblasts was disturbed. We also found that ameloblasts of Nrf 2-null mouse show degenerative atrophy at the late maturation stage, which gives rise to the loss of iron deposition to the surface of mature enamel. Our results thus demonstrate that the enamel organ of Nrf 2-deficient mouse has a reduced iron transport capacity, which results in both the enamel cell degeneration and disturbance of iron deposition on to the enamel surface.

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Pigmentation of the enamel of albino rat incisor teeth

Cited by 29 publications

References 6 publications

Iron Deposition and Ferritin Heavy Chain (Fth) Localization in Rodent Teeth

Iron Deposition and Ferritin Heavy Chain (Fth) Localization in Rodent Teeth

Cytochemical Studies of Ameloblasts and the Surface Layer of Enamel of the Rat Incisor at the Maturation Stage

Nrf2 deficiency causes tooth decolourization due to iron transport disorder in enamel organ

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