Iron Deposition and Ferritin Heavy Chain (Fth) Localization in Rodent Teeth

Abstract

BackgroundAn iron rich layer on the labial surface is characteristic of the enamel of rodent incisors. In order to address a role for iron content in continuously growing incisors during odontogenesis, we studied iron deposition patterns in enamel and dentine using Perls’ blue staining and ferritin heavy chain (Fth) immunolocalization. Fth expression is regulated by iron level; therefore its localization can be used as a sensitive indicator for iron deposition.ResultsSagittal sections of 4-week old rat incisors showed a gradual increase in iron level in the enamel organ from secretory to maturation stages. In addition, iron was detected in ameloblasts of erupting third molars of 4-week old rats, suggesting iron plays a role in both incisor and molar development. In odontoblasts, the presence of iron was demonstrated, and this is consistent with iron’s role in collagen synthesis. Using postnatal 3-, 6-, 9-day old mice, the spatial and temporal expression of Fth in tooth development again indicated the presence of iron in mature ameloblasts and odontoblasts.ConclusionsWhile these data do not explain what functional role iron has in tooth formation, it does highlight a significant molecular activity associated with the formation of the rodent dentition.

U50: A New Metric for Measuring Assembly Output Based on Non-Overlapping, Target-Specific Contigs

“…This is because NG 50 estimates the reference genome length based on the input contigs themselves (Ghodsi et al 2013), not on an input reference genome. This exemplifies that the overinflated NG 50 results can be inaccurate and misleading.…”

Stains on primary teeth- a review

“…(12,13) Iron deficiency affects more than two billion people in the world and is one of the most common nutritional deficiencies. (14) Although iron deficiency may sometimes result from defects in the body's ability to metaboliseiron, the most common cause is inadequate iron in the diet, a deficiency that is treatable by foods and supplements which contain iron salts.…”

Tranexamic acid

“…[56-61] Recent retrospective studies have not shown evidence of increased thromboembolic events. Larger randomized control trials are still needed to determine the ideal dosing and timing of TXA administration in this population.…”