Piezo-Augmented Sonosensitizer with Strong Ultrasound-Propelling Ability for Efficient Treatment of Osteomyelitis

Abstract

The successful treatment of osteomyelitis remains a great challenge in the field of orthopedics. The clinical method for treating refractory bone infection requires a combination therapy of long-term systemic antibiotics administration and surgical debridement. It is highly desirable to develop an antibiotic-free, noninvasive, rapid strategy to eradicate osteomyelitis. Herein, we fabricate a piezoelectricenhanced sonosensitizer that consists of a porphyrin-based hollow metal−organic framework (HNTM), MoS 2 nanosheets, and a red cell (RBC) membrane. We find that the ultrasound (US)-induced piezoelectric polarization of MoS 2 can improve the charge transfer of HNTM at the heterointerface of HNTM-MoS 2 , increasing the production of reactive oxygen species (ROS). Besides, MoS 2 increases the asymmetric shape of HNTM, leading to the strong US-propelling ability of HNTM−MoS 2 . The produced ROS and strong mechanical force can kill methicillin-resistant Staphylococcus aureus (MRSA) with an antibacterial efficiency of 98.5% under 15 min of US treatment, resulting in intracellular DNA damage and increased oxidative stress and disturbance of purine metabolism, tryptophan metabolism, and pantothenate and CoA biosynthesis of MRSA. Together with the toxin neutralization ability, the RBC-HNTM-MoS 2 successfully eliminates the bone infection and suppresses inflammation and bone loss. This work provides another strategy for developing an efficient sonosensitizer through piezoelectric-assisted sonocatalysis and enhancing US-propelling ability.

Nanomaterial-assisted theranosis of bone diseases

“…Feng et al synthesized nanocomposites consisting of hollow metal-organic framework (HNTM), MoS 2 nanosheets and a red cell (RBC) membrane for the efficient treatment of osteomyelitis ( Fig. 27 A) [ 303 ]. The RBC-HNTM-MoS 2 exhibited strongly antibacterial efficiency of 98.5% against MRSA under 15 min ultrasound treatment.…”

“…A) Sonocatalytic mechanism of HNTM-MoS 2 and the efficient SDT treatment of osteomyelitis through rapid MRSA elimination and detoxification. (i) HRTEM images of HNTM, MoS 2 nanosheets, and HNTM-MoS 2 [ 303 ]. The images reproduced with the permission from American Chemical Society.…”

Research Trends of Piezoelectric Nanomaterials in Biomedical Engineering

“…Contrast and inhibition of tumors [90] Au@BTO A piezoelectric effect is produced under ultrasound conditions Antibacterial and promotes wound healing [92] RBC-HNTM-MoS 2 Ultrasonically induced piezoelectric effects enhance ultrasonic propulsion capability Antibacterial and inhibits bone loss [93] PVDF/4Ag-PBT Ultrasound-induced piezoelectric effect, releasing silver ions. Exhibits good antibacterial activity against Escherichia coli.…”

“…Feng et al fabricated a composite structure (RBC‐HNTM‐MoS 2 ) composed of a hollow metal–organic framework (HNTM), MoS 2 nanosheets, and red cells (RBC) for the SDT of osteomyelitis (Figure 7b). [ 93 ] MoS 2 nanosheets, as piezoelectric materials, form heterojunctions with the HNTM. The efficiency of ROS formation could be improved using ultrasonically induced piezoelectric polarization at the heterogeneous interface of HNTM‐MoS 2 .…”

“…Copyright 2022, American Chemical Society. b) Reproduced with permission [93]. Copyright 2020, Elsevier.…”