PI(4,5)P2 forms dynamic cortical structures and directs actin distribution as well as polarity in <i>C. elegans</i> embryos

Scholze, Melina J.; Barbieux, Kévin; Simone, Alessandro De; Boumasmoud, Mathilde; Süess, Camille C. N.; Wang, Ruijia; Gönczy, Pierre

doi:10.1242/dev.164988

Cited by 18 publications

(29 citation statements)

References 92 publications

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“…The asymmetric distribution of plasma membrane PIP2 along the antero-posterior axis was confirmed recently and found to be dependent upon PAR determinants and F-actin (Scholze et al, 2018). Interestingly, PIP2 reciprocally regulates F-actin enrichment at one pole, which also appears to be required for proper polarity establishment and spindle positioning (Scholze et al, 2018).…”

Section: Lipids and Asymmetric Cell Divisionmentioning

confidence: 72%

“…In addition, PPK-1, a PI(4)P5 kinase, was found to accumulate at the posterior side of the one-cell embryo, where it would be responsible for an asymmetric generation of phosphatidylinositol-4,5-bisphosphate (PIP2), which in turn would lead to the recruitment at the posterior of LIN-5 and GPR-1/2, two factors required for asymmetric spindle positioning, in an unknown manner (Panbianco et al, 2008). The asymmetric distribution of plasma membrane PIP2 along the antero-posterior axis was confirmed recently and found to be dependent upon PAR determinants and F-actin (Scholze et al, 2018). Interestingly, PIP2 reciprocally regulates F-actin enrichment at one pole, which also appears to be required for proper polarity establishment and spindle positioning (Scholze et al, 2018).…”

Section: Lipids and Asymmetric Cell Divisionmentioning

confidence: 89%

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Lipid Mediated Regulation of Adult Stem Cell Behavior

Clémot

Demarco

Jones

2020

Front. Cell Dev. Biol.

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Adult stem cells constitute an important reservoir of self-renewing progenitor cells and are crucial for maintaining tissue and organ homeostasis. The capacity of stem cells to self-renew or differentiate can be attributed to distinct metabolic states, and it is now becoming apparent that metabolism plays instructive roles in stem cell fate decisions. Lipids are an extremely vast class of biomolecules, with essential roles in energy homeostasis, membrane structure and signaling. Imbalances in lipid homeostasis can result in lipotoxicity, cell death and diseases, such as cardiovascular disease, insulin resistance and diabetes, autoimmune disorders and cancer. Therefore, understanding how lipid metabolism affects stem cell behavior offers promising perspectives for the development of novel approaches to control stem cell behavior either in vitro or in patients, by modulating lipid metabolic pathways pharmacologically or through diet. In this review, we will first address how recent progress in lipidomics has created new opportunities to uncover stem-cell specific lipidomes. In addition, genetic and/or pharmacological modulation of lipid metabolism have shown the involvement of specific pathways, such as fatty acid oxidation (FAO), in regulating adult stem cell behavior. We will describe and compare findings obtained in multiple stem cell models in order to provide an assessment on whether unique lipid metabolic pathways may commonly regulate stem cell behavior. We will then review characterized and potential molecular mechanisms through which lipids can affect stem cell-specific properties, including self-renewal, differentiation potential or interaction with the niche. Finally, we aim to summarize the current knowledge of how alterations in lipid homeostasis that occur as a consequence of changes in diet, aging or disease can impact stem cells and, consequently, tissue homeostasis and repair.

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Section: Lipids and Asymmetric Cell Divisionmentioning

confidence: 72%

Section: Lipids and Asymmetric Cell Divisionmentioning

confidence: 89%

Lipid Mediated Regulation of Adult Stem Cell Behavior

Clémot

Demarco

Jones

2020

Front. Cell Dev. Biol.

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“…Asymmetric enrichment of phosphatidylinositol 4,5-bisphosphate (PIP 2 ), has been observed within another class of membrane-associated domains in the anterior of the C. elegans zygote (Nakayama et al, 2009; Wang et al, 2017; Scholze et al, 2018). Similar enrichment is seen for the polarity-related Rho-family GTPases CDC-42 and RHO-1, the RHO-1 regulator ECT-2, a CDC-42-associated sub-population of PAR-6 and PKC-3, and casein kinase (CSNK-1) (Motegi and Sugimoto, 2006; Schonegg et al, 2007; Panbianco et al, 2008; Wang et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

“…Similar enrichment is seen for the polarity-related Rho-family GTPases CDC-42 and RHO-1, the RHO-1 regulator ECT-2, a CDC-42-associated sub-population of PAR-6 and PKC-3, and casein kinase (CSNK-1) (Motegi and Sugimoto, 2006; Schonegg et al, 2007; Panbianco et al, 2008; Wang et al, 2017). PIP 2 -enriched microdomains have been proposed to serve as organizing platforms to coordinate regulation of cortical actin organization, cell polarity and asymmetric division of the zygote (Scholze et al, 2018). Despite being noted over a decade ago, the nature of these domains remains poorly understood.…”

Section: Introductionmentioning

confidence: 99%

Anterior-enriched filopodia create the appearance of asymmetric membrane microdomains in polarizing C. elegans zygotes

Hirani

Illukkumbura

Bland

et al. 2019

Journal of Cell Science

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The association of molecules within membrane microdomains is critical for the intracellular organization of cells. During polarization of the C. elegans zygote, both polarity proteins and actomyosin regulators associate within dynamic membrane-associated foci. Recently, a novel class of asymmetric membrane-associated structures was described that appeared to be enriched in phosphatidylinositol 4,5-bisphosphate (PIP 2 ), suggesting that PIP 2 domains could constitute signaling hubs to promote cell polarization and actin nucleation. Here, we probe the nature of these domains using a variety of membrane- and actin cortex-associated probes. These data demonstrate that these domains are filopodia, which are stimulated transiently during polarity establishment and accumulate in the zygote anterior. The resulting membrane protrusions create local membrane topology that quantitatively accounts for observed local increases in the fluorescence signal of membrane-associated molecules, suggesting molecules are not selectively enriched in these domains relative to bulk membrane and that the PIP 2 pool as revealed by PH PLCδ1 simply reflects plasma membrane localization. Given the ubiquity of 3D membrane structures in cells, including filopodia, microvilli and membrane folds, similar caveats are likely to apply to analysis of membrane-associated molecules in a broad range of systems.

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“…The protein‐lipid interaction is an important part of regulating the membranous structure for cellular activity expressions. In eukaryotic cells, the Pleckstrin homology domain in phospholipase C‐δ1 produces a pulling force of membranous structure to form a membrane compartment through the interaction with phosphorylated phosphatidylinositols . The proteins possessing Bin/amphiphysin/Rvs (BAR) domain also bound to phosphatidylinositols and the diverse BAR protein family including I‐BAR and F‐BAR have an important role for membrane invagination processes .…”

Section: Introductionmentioning

confidence: 99%

Enhanced Tubulation of Liposome Containing Cardiolipin by MamY Protein from Magnetotactic Bacteria

Tanaka

Suwatthanarak

Arakaki

et al. 2018

Biotechnology Journal

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Lipid tubules are of particular interest for many potential applications in nanotechnology. Among various lipid tubule fabrication techniques, the morphological regulation of membrane structure by proteins mimicking biological processes may provide the chances to form lipid tubes with highly tuned structures. Magnetotactic bacteria synthesize magnetosomes (a unique prokaryotic organelle comprising a magnetite crystal within a lipid envelope). MamY protein is previously identified as the magnetosome protein responsible for magnetosome vesicle formation and stabilization. Furthermore, MamY is shown in vitro liposome tubulation activity. In this study, the interaction of MamY and phospholipids is investigated by using a lipids-immobilized membrane strip and a peptide array. Here, the binding of MamY to the anionic phospholipid, cardiolipin, is found and enhanced liposome tubulation efficiency. The authors propose the interaction is responsible for recruiting and locating cardiolipin to elongate liposome in vitro. The authors also suggest a similar mechanism for the invagination site in magnetosomes vesicle formation, where the lipid itself contributes further to increasing the curvature. These findings are highly important to develop an effective biomimetic synthesis technique of lipid tubules and to elucidate the unique prokaryotic organelle formation in magnetotactic bacteria.

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PI(4,5)P2 forms dynamic cortical structures and directs actin distribution as well as polarity in C. elegans embryos

Cited by 18 publications

References 92 publications

Lipid Mediated Regulation of Adult Stem Cell Behavior

Lipid Mediated Regulation of Adult Stem Cell Behavior

Anterior-enriched filopodia create the appearance of asymmetric membrane microdomains in polarizing C. elegans zygotes

Enhanced Tubulation of Liposome Containing Cardiolipin by MamY Protein from Magnetotactic Bacteria

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