Phytotoxic and antioxidant effects of dichloromethane fraction of Smilax Brasiliensis Sprengel

Abstract

Dichloromethane (DCM) fraction and sub-fractions obtained from Smilax brasiliensis leaves were examined in order to determine their phytotoxic and antioxidant effects. The dichloromethane fraction was submitted to a preparative layer chromatography leading to seven sub-fractions (DCM1-DCM7). Gas chromatography-mass spectrometry (GC-MS) was performed on the dichloromethane sub-fractions. The DCM sub-fractions presented phytotoxic potential; at a concentration of 125 µg per plate, DCM6 and DCM4 showed the strongest results on Lactuca sativa and Allium cepa, respectively. The DCM fraction and DCM4 sub-fraction were more effective than 2,6-di-tert-butyl-4-methylphenol (BHT) at scavenging the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical.Analysis by GC-MS showed the presence of methyl palmitate (33.05%) in DCM4 and methyl palmitate (17.29%) and methyl oleate (50.96%) in DCM6, suggesting that the activities exhibited by the sub-fractions may be attributed, at least partially, to these major compounds. These results indicate that the DCM sub-fractions of S. brasiliensis could be used as natural herbicides and antioxidants.

Influence of the methanol extract and fractions of Smilax brasiliensis Sprengel on development in vitro of Nicotiana tabacum and Alllium cepa

“…Allelopathic, cytotoxic, genotoxic and antigenotoxic effects were observed in the studies of Amado et al (2019Amado et al ( , 2020a. The phytotoxic effects of the hexane and dichloromethane fractions from the leaves of S. brasiliensis was also demonstrated (Barbosa et al, 2021;Fonseca et al, 2019). This work aimed to evaluate the influence of the extract and fractions S. brasiliensis leaves on development in vitro of Nicotiana tabacum and Allium cepa seeds.…”

Efeitos da extração hidroetanolica e sequencial sobre o potencial tóxico dos extratos de diferentes partes da Parkia platycephala do Cerrado tocantinense

“…Os extratos hexânico e hidroetanolico da folha da espécie Caryocar coriaceum (pequizeiro) inibiu o crescimento radicular da A. cepa na concentração de 50 µg/ml (Cardoso et al, 2021). Barbosa et al (2019) avaliaram a toxicidade da fração e subfrações de diclorometano (DCM) obtidas das folhas de Smilax brasiliensis (japecanga) e observaram o efeito tóxico contra a Lactuca sativa e Allium cepa.…”