Phytosynthesis and Characterization of Copper Oxide Nanoparticles using the Aqueous Extract of Beta vulgaris L and Evaluation of their Antibacterial and Anticancer Activities

Chandrasekaran, Rajkuberan; Yadav, Sangilimuthu Alagar; Sivaramakrishnan, S.

doi:10.1007/s10876-019-01640-6

Cited by 47 publications

(20 citation statements)

References 33 publications

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“…However, the most antibacterial effect of Co 3 O 4 NPs was observed on S. aureus and E. coli, referring to more changes in the outer membrane of Gram-positive bacteria in the presence of cobalt ions. Similar to our results, previous studies have reported the antibacterial activities of copper oxide, 66,67 cerium oxide, 62 zinc oxide 68,69 and zero-valent iron 38 NPs. In addition, Chang et al, 70 Parada et al 53 and Khan et al 52 showed that by increasing the concentration of Co 3 O 4 NPs up to 200 µg/mL., the activity of both Gram-positive and Gram-negative bacteria were significantly decreased.…”

Section: Discussionsupporting

confidence: 93%

Exploring the Interaction of Cobalt Oxide Nanoparticles with Albumin, Leukemia Cancer Cells and Pathogenic Bacterial by Multispectroscopic, Docking, Cellular and Antibacterial Approaches

Arsalan

Kashi

Hasan

et al. 2020

IJN

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The interaction of NPs with biological systems may reveal useful details about their pharmacodynamic, anticancer and antibacterial effects. Methods: Herein, the interaction of as-synthesized Co 3 O 4 NPs with HSA was explored by different kinds of fluorescence and CD spectroscopic methods, as well as molecular docking studies. Also, the anticancer effect of Co 3 O 4 NPs against leukemia K562 cells was investigated by MTT, LDH, caspase, real-time PCR, ROS, cell cycle, and apoptosis assays. Afterwards, the antibacterial effects of Co 3 O 4 NPs against three pathogenic bacteria were disclosed by antibacterial assays. Results: Different characterization methods such as TEM, DLS, zeta potential and XRD studies proved that fabricated Co 3 O 4 NPs by sol-gel method have a diameter of around 50 nm, hydrodynamic radius of 177 nm with a charge distribution of −33.04 mV and a welldefined crystalline phase. Intrinsic, extrinsic, and synchronous fluorescence as well as CD studies, respectively, showed that the HSA undergoes some fluorescence quenching, minor conformational changes, microenvironmental changes as well as no structural changes in the secondary structure, after interaction with Co 3 O 4 NPs. Molecular docking results also verified that the spherical clusters with a dimension of 1.5 nm exhibit the most binding energy with HSA molecules. Anticancer assays demonstrated that Co 3 O 4 NPs can selectively lead to the reduction of K562 cell viability through the cell membrane damage, activation of caspase-9,-8 and-3, elevation of Bax/Bcl-2 mRNA ratio, ROS production, cell cycle arrest, and apoptosis. Finally, antibacterial assays disclosed that Co 3 O 4 NPs can stimulate a promising antibacterial effect against pathogenic bacteria. Conclusion: In general, these observations can provide useful information for the early stages of nanomaterial applications in therapeutic platforms.

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Section: Discussionsupporting

confidence: 93%

Exploring the Interaction of Cobalt Oxide Nanoparticles with Albumin, Leukemia Cancer Cells and Pathogenic Bacterial by Multispectroscopic, Docking, Cellular and Antibacterial Approaches

Arsalan

Kashi

Hasan

et al. 2020

IJN

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“…Numerous studies have reported the anticancer effect of plant-mediated Cu/CuO NPs, particularly breast [ 37 , 53 , 54 , 66 , 78 , 81 , 111 , 112 ], cervical [ 53 , 54 , 79 ], colon [ 37 ], skin (epithelioma) [ 54 ], gastric [ 86 ], blood (leukemia) [ 37 ], liver [ 65 ], lung [ 54 , 56 , 69 , 70 ] and ovarian [ 78 ] cancers ( Table 4 ).…”

Section: Application Of Cu and Cuo Npsmentioning

confidence: 99%

“…The exposure of NPs promotes DNA damage that eventually halts the cell cycle. Biofabrication of CuO NPs using E. globulus leaf extract and Beta vulgaris aqueous extract induced cell cycle arrest at the G2/M phase in MCF-7 breast cancer cells and A549 cancer cells, respectively [ 56 , 66 ]. Additionally, the expression of p53 was upregulated [ 66 ].…”

Section: Application Of Cu and Cuo Npsmentioning

confidence: 99%

“…Numerous studies have reported the anticancer effect of plant-mediated Cu/CuO NPs, particularly breast [37,53,54,66,78,81,111,112], cervical [53,54,79], colon [37], skin (epithelioma) [54], gastric [86], blood (leukemia) [37], liver [65], lung [54,56,69,70] and ovarian [78] cancers (Table 4). Alternatively, the antifungal activity of CuO NPs may be attributed to their antioxidant property.…”

Section: Anticancermentioning

confidence: 99%

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Plant-Based Biosynthesis of Copper/Copper Oxide Nanoparticles: An Update on Their Applications in Biomedicine, Mechanisms, and Toxicity

et al. 2021

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Plants are rich in phytoconstituent biomolecules that served as a good source of medicine. More recently, they have been employed in synthesizing metal/metal oxide nanoparticles (NPs) due to their capping and reducing properties. This green synthesis approach is environmentally friendly and allows the production of the desired NPs in different sizes and shapes by manipulating parameters during the synthesis process. The most commonly used metals and oxides are gold (Au), silver (Ag), and copper (Cu). Among these, Cu is a relatively low-cost metal that is more cost-effective than Au and Ag. In this review, we present an overview and current update of plant-mediated Cu/copper oxide (CuO) NPs, including their synthesis, medicinal applications, and mechanisms. Furthermore, the toxic effects of these NPs and their efficacy compared to commercial NPs are reviewed. This review provides an insight into the potential of developing plant-based Cu/CuO NPs as a therapeutic agent for various diseases in the future.

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“…Change in the color of the reaction mixture is a response of the interaction of nanoparticles of different sizes and shapes to different electromagnetic light waves. 35 An increase in the concentration of AgNO 3 resulted in increasing the ionic concentration of Ag that may function to affect the enzymatic conformations and electronegative states of reactants resulted in the change of the color of the reaction mixture (very light brown). Surface plasmon resonance (SPR) band which is a result of the vibration of free electrons of AgNPs in coordination with the metal lattice in resonance and the oscillating electromagnetic field of light waves, was observed 36 (Figure 1B).…”

Section: Synergistic Effects Of Different Physicochemical Reaction Comentioning

confidence: 99%

Synergistic Effects of Physicochemical Parameters on Bio-Fabrication of Mint Silver Nanoparticles: Structural Evaluation and Action Against HCT116 Colon Cancer Cells

Javed¹,

Mashwani²

2020

IJN

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Background: Physicochemical parameters such as temperature, pH, the concentration of the AgNO 3 and ratio of reactants act synergistically to influence the reaction kinetics, molecular mechanics, enzymatic catalysis and protein conformations that aid to affect the size, shape and biochemical corona of nanoparticles. The present study was performed to investigate the influence of reaction parameters on the bio-fabrication of silver nanoparticles (AgNPs) by using Mentha arvensis and to determine their potential to control the proliferation of colon cancer cells'. Methods: Plant-mediated method was used for the bio-fabrication and stabilization of AgNPs. Reaction parameters were arranged, and surface plasmon resonance (SPR) bands of AgNPs were collected by using a UV-Visible spectrophotometer. NPs were characterized structurally and optically by using SEM, AFM, EDX and DLS techniques. AgNPs and plant aqueous extract were tested against HCT116 colon cancer cells by using SRB assay, Annexin V assay and cell cycle analysis. Results: Spectrophotometric comparison of various reaction conditions manifested that 5 mM of AgNO 3 , 60°C in an acidic pH and a mixing ratio of 1:9 of plant extract and AgNO 3 , respectively, are the optimized conditions for AgNP synthesis. Structural evaluation by SEM, AFM and particle size analysis confirmed that the NPs are <100 nm and are anisotropic, spherical, triangular and moderately dispersed in the colloidal mixture. SRB assay expressed biomass-stabilized AgNPs as effective cytotoxic particles against HCT116 colon cancer cells, and the IC 50 was measured at 1.7 µg/mL. Annexin V apoptosis assay further confirmed that the AgNPs induce apoptosis in a dose-dependent manner. Experimental evidence manifested that the AgNPs arrest cell cycle and expressed entrapment of a greater number of cells in the Sub-G1 phase, further verifying the anticancer abilities of AgNPs. Conclusion: These findings explain the synergistic effects of physicochemical parameters to optimize the phytosynthesis of biocompatible AgNPs to overcome the limitations of conventional chemotherapeutic treatments of colon cancer cells.

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Phytosynthesis and Characterization of Copper Oxide Nanoparticles using the Aqueous Extract of Beta vulgaris L and Evaluation of their Antibacterial and Anticancer Activities

Cited by 47 publications

References 33 publications

<p>Exploring the Interaction of Cobalt Oxide Nanoparticles with Albumin, Leukemia Cancer Cells and Pathogenic Bacterial by Multispectroscopic, Docking, Cellular and Antibacterial Approaches</p>

<p>Exploring the Interaction of Cobalt Oxide Nanoparticles with Albumin, Leukemia Cancer Cells and Pathogenic Bacterial by Multispectroscopic, Docking, Cellular and Antibacterial Approaches</p>

Plant-Based Biosynthesis of Copper/Copper Oxide Nanoparticles: An Update on Their Applications in Biomedicine, Mechanisms, and Toxicity

<p>Synergistic Effects of Physicochemical Parameters on Bio-Fabrication of Mint Silver Nanoparticles: Structural Evaluation and Action Against HCT116 Colon Cancer Cells</p>

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