PhytopathogenicBurkholderiaSpecies in Bulb Plants in Bulgaria

Isolation and identification of Burkholderia gladioli on Cymbidium orchids in Korea

“…existent [17,18]. In the present study, the isolate 2-9 was identified as B. gladioli by PCR analysis using species-specific primers and the sequence analysis of the 16S rDNA gene.…”

“…AGAGTTTGATCMTGGCTCAG-3') and G16-2 (5'-CGAAG-GATATTAGCCCTC-3') [16][17][18], GLA-f (5'-CGAGCTAA-TACCGCGAAA-3') and GLA-r (5'-AGACTCGAGTCAACTGA-3') [19].…”

“…The isolates were identified by PCR amplification using species-specific primers. Previously, one of the species-specific primer set, CMG16-1 and G16-2, was developed to detect the presence of the specific gene regions in the area coding 16S rDNA of B. gladioli [16][17][18]27]. We used the primer set and successfully amplified an approximately 470-bp fragment of 16S rDNA from B. gladioli pv.…”

“…Bgg has very wide host ranges including gladioli, freesia, irises and Dendrobium [1]. On the contrary, Bga, which infects tulip and onion [1], is well known to cause 'slippery skin' on onion bulbs and dry necrotic spots on onion leaves [15,17,18]. The first report in Korea of soft rot of onion by Bga was in the summer of 1999 [15], and it is now becoming a common disease during storage or transit.…”

“…Taken together, our results indicate that the isolate 2-9 is pathogenic to Cymbidium but not to onion, suggesting that the isolate 2-9 could be Bgg. However, although onion plants are common host plants for Bga, there are a few reports that Bgg is isolated from diseased onions [17][18][19]. Therefore, although isolate 2-9 is most likely Bgg, the interpretation of the results from the pathogenicity test should be viewed with caution.…”