Isolation and identification of <i>Burkholderia gladioli</i> on <i>Cymbidium</i> orchids in Korea

Moon, Hyeonjoon; Park, Hye-Jee; Jeong, Aram; Han, Sang-Wook; Park, Chang-Jin

doi:10.1080/13102818.2016.1268069

Cited by 13 publications

(4 citation statements)

References 26 publications

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“…However, the orchid industry suffers financially from poor crop yields due to the problem of plant diseases. Most orchid diseases are acquired through infection from bacteria [ 20 ], viruses [ 21 ], and fungi [ 22 ]. Thus, controlling disease is an important practice for maintaining the quality of orchids.…”

Section: Introductionmentioning

confidence: 99%

Biocontrol of Orchid-pathogenic Mold,Phytophthora palmivora, by Antifungal Proteins fromPseudomonas aeruginosaRS1

Sowanpreecha

Rerngsamran

2018

Mycobiology

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Black rot disease in orchids is caused by the water mold Phytophthora palmivora. To gain better biocontrol performance, several factors affecting growth and antifungal substance production by Pseudomonas aeruginosa RS1 were verified. These factors include type and pH of media, temperature, and time for antifungal production. The results showed that the best conditions for P. aeruginosa RS1 to produce the active compounds was cultivating the bacteria in Luria-Bertani medium at pH 7.0 for 21 h at 37 °C. The culture filtrate was subjected to stepwise ammonium sulfate precipitation. The precipitated proteins from the 40% to 80% fraction showed antifungal activity and were further purified by column chromatography. The eluted proteins from fractions 9–10 and 33–34 had the highest antifungal activity at about 75% and 82% inhibition, respectively. SDS-PAGE revealed that the 9–10 fraction contained mixed proteins with molecular weights of 54 kDa, 32 kDa, and 20 kDa, while the 33–34 fraction contained mixed proteins with molecular weights of 40 kDa, 32 kDa, and 29 kDa. Each band of the proteins was analyzed by LC/MS to identify the protein. The result from Spectrum Modeler indicated that these proteins were closed similarly to three groups of the following proteins; catalase, chitin binding protein, and protease. Morphological study under scanning electron microscopy demonstrated that the partially purified proteins from P. aeruginosa RS1 caused abnormal growth and hypha elongation in P. palmivora. The bacteria and/or these proteins may be useful for controlling black rot disease caused by P. palmivora in orchid orchards.

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Section: Introductionmentioning

confidence: 99%

Biocontrol of Orchid-pathogenic Mold,Phytophthora palmivora, by Antifungal Proteins fromPseudomonas aeruginosaRS1

Sowanpreecha

Rerngsamran

2018

Mycobiology

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“…The amplified fragments were cloned into pNC-Cam1304 binary vectors (NC biotech, Hainan, China) for gene overexpression. The corresponding constructs were then infiltrated into 2-year-old D. catenatum leaves following an Agrobacterium -mediated method [ 36 ] (Fig. S 1 ).…”

Section: Methodsmentioning

confidence: 99%

Genome-wide identification and characterization of active ingredients related β-Glucosidases in Dendrobium catenatum

et al. 2022

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Background Dendrobium catenatum/D. officinale (here after D. catenatum), a well-known economically important traditional medicinal herb, produces a variety of bioactive metabolites including polysaccharides, alkaloids, and flavonoids with excellent pharmacological and clinical values. Although many genes associated with the biosynthesis of medicinal components have been cloned and characterized, the biosynthetic pathway, especially the downstream and regulatory pathway of major medicinal components in the herb, is far from clear. β-glucosidases (BGLUs) comprise a diverse group of enzymes that widely exist in plants and play essential functions in cell wall modification, defense response, phytohormone signaling, secondary metabolism, herbivore resistance, and scent release by hydrolyzing β-D-glycosidic bond from a carbohydrate moiety. The recent release of the chromosome-level reference genome of D. catenatum enables the characterization of gene families. Although the genome-wide analysis of the BGLU gene family has been successfully conducted in various plants, no systematic analysis is available for the D. catenatum. We previously isolated DcBGLU2 in the BGLU family as a key regulator for polysaccharide biosynthesis in D. catenatum. Yet, the exact number of DcBGLUs in the D. catenatum genome and their possible roles in bioactive compound production deserve more attention. Results To investigate the role of BGLUs in active metabolites production, 22 BGLUs (DcBGLU1-22) of the glycoside hydrolase family 1 (GH1) were identified from D. catenatum genome. Protein prediction showed that most of the DcBGLUs were acidic and phylogenetic analysis classified the family into four distinct clusters. The sequence alignments revealed several conserved motifs among the DcBGLU proteins and analyses of the putative signal peptides and N-glycosylation site revealed that the majority of DcBGLU members dually targeted to the vacuole and/or chloroplast. Organ-specific expression profiles and specific responses to MeJA and MF23 were also determined. Furthermore, four DcBGLUs were selected to test their involvement in metabolism regulation. Overexpression of DcBGLU2, 6, 8, and 13 significantly increased contents of flavonoid, reducing-polysaccharide, alkaloid and soluble-polysaccharide, respectively. Conclusion The genome-wide systematic analysis identified candidate DcBGLU genes with possible roles in medicinal metabolites production and laid a theoretical foundation for further functional characterization and molecular breeding of D. catenatum.

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“…Therefore, when appropriate environmental conditions occur, this bacterium could become established in corn crops (Lu et al., 2007), and the climate in Thailand is suitable for the occurrence of these bacterial disease (Parkpoom & Donnua, 2019). Initially, B. gladioli was identified as a gladiolus pathogen (Mcculloch, 1921; Moon et al., 2017). The symptoms on sweet corn were circular shaped, water‐soaked, rotted and necrotic tissue at the base of stalk.…”

Section: Figurementioning

confidence: 99%

Characterization and identification of Burkholderia gladioli causing stalk rot of sweet corn in Thailand

Chiangkhaek,

Akarapisan

2024

Journal of Phytopathology

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Stalk rot disease is becoming an important disease in sweet corn crops because it causes significant yield losses of the crop. In July 2022, typical symptoms of stem rot were observed in fields of Mae Hia Agricultural Research, Demonstration and Training Center in Chiang Mai province of Thailand. The symptoms exhibited by affected crop included circular water‐soaked lesions, rot and necrotic tissue at the base of the stalk. Koch's postulates were fulfilled with reisolation of the inoculated strain from the inoculated tissues, and confirmation was obtained by phenotypic characters and 16S rRNA sequences. The isolated colony on nutrient agar was cream‐coloured, circular, with a convex elevation, entire margin and smooth surface; the optical character was translucent and exuded a yellowish pigment that diffused in the nutrient agar, gram‐negative, lacked arginine dihydrolase, occurrence of the oxidase and catalase reaction. The strains were able to utilize d‐galactose and glucose but were unable to utilize d‐maltose, d‐cellobiose, d‐fructose, d‐mannose, mannitol, lactose and sucrose. The strains showed positive results for gelatine liquefaction test, citrate utilization and casein hydrolysis, but starch hydrolysis and indole production were negative result. These characteristics are the same as those described previously for Burkholderia gladioli. Disease severity in sweet corn was 43.8%, with infections showing spots with dark margins and soft rot on mature leaves and flag leaves. Disease severity in waxy corn and maize were 21.9% and 15.6%, respectively, showing a few light or dark brown spots at the infection sites. Therefore, this represents the first report of leaf stripe and stalk rot disease in sweet corn in Thailand.

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Isolation and identification of Burkholderia gladioli on Cymbidium orchids in Korea

Cited by 13 publications

References 26 publications

Biocontrol of Orchid-pathogenic Mold,Phytophthora palmivora, by Antifungal Proteins fromPseudomonas aeruginosaRS1

Biocontrol of Orchid-pathogenic Mold,Phytophthora palmivora, by Antifungal Proteins fromPseudomonas aeruginosaRS1

Genome-wide identification and characterization of active ingredients related β-Glucosidases in Dendrobium catenatum

Characterization and identification of Burkholderia gladioli causing stalk rot of sweet corn in Thailand

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