Phytopathogenic Bacteria in the Pathology of Forest Trees of Polyssya and Forest-Steppe of Ukraine

Goychuk, Anatoliy; Drozda, Valentin; Kulbanska, Ivanna; Shvets, М.V.

doi:10.31521/2313-092x/2019-2(102)-4

Cited by 3 publications

(2 citation statements)

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“…Currently, eight independent families are distinguished within the order of Enterobacterales, and the Erwinia genus is included in the Erwiniaceae family [14]. Erwinia nimipressuralis was indicated as a causative agent of hardwood bacterial dropsy in Ukraine [17] and coniferous species in Central Siberia [10]. The species Erwinia nimipressuralis was believed to affect beech and fir, while Erwinia multivora was considered to influence only birch.…”

Section: Discussionmentioning

confidence: 99%

Detection of Phytopathogenic Bacteria Damaging Weeping Birch (Betula Pendula) by Molecular Identification Method

Baubekova¹,

Abiyev²,

Asilkhanova³

et al. 2022

Pol. J. Environ. Stud.

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The article discusses the morphological changes of birch trees due to bacterial dropsy growing in the "Green Belt" artificial forest in the surrounding of the Nur-Sultan city. Samples of kernel were taken from diseased tree trunks, and, afterward, isolated bacterial cultures were grown in a nutrient medium. For molecular genetic studies, one strain (designated as DDdd-1), the most typical of morphological characteristics, was selected, the colonies of which showed the greatest growth on nutrient media. The nucleotide sequences 8F and 806R of 16SrRNA were determined on the basis of sequencing. The resulting sequences were compared in GenBank from the NCBI database showing related species and their similarities. Extraction of cores from infected birch trunks, isolation of pure strains of the pathogen, molecular identification of ribosomal RNA 16S nucleotide chains were performed using modern methods in the field of bacteriology. Then, the results obtained were compared with relevant data from the International Genbank database. Molecular characteristics of isolated bacterial DDdd-1 strain have been found to correspond to typical Dickeya dadantii by 96.72%. According to the International Genbank, the DDdd-1 strain is defined as pathovar D. dadantii pv. betulae. The data obtained demonstrate close relation of DDdd-1 strain Dickeya dadantii pv. betulae to some strains of Dickeya chrysanthemi. The species identified in International Genbank as Erwinia chrysanthemi with index numbers KJ541470.1 and AF373175.1 are assumed to be identical to D. dadantii pv. betulae. Dickeya is a complex taxon that still requires a lot of research efforts on species composition, close family bonds, the size and structure of host plants.

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Section: Discussionmentioning

confidence: 99%

Detection of Phytopathogenic Bacteria Damaging Weeping Birch (Betula Pendula) by Molecular Identification Method

Baubekova¹,

Abiyev²,

Asilkhanova³

et al. 2022

Pol. J. Environ. Stud.

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“…Mico-and microorganisms are an integral part of the forest biocenosis, which determines its depth, which is directly involved in all stages of growth and development of woody plants and the utilization of mortmass and detritus. Among the systematic and functional groups of myco-and microbiota, a special place is occupied by phytopathogenic endophytes of various trophic specializations, capable of causing significant ecological, economic and social damage under certain conditions, which is confirmed by deep pathology 2013), bacteria (Cherpakov, 2012;Goychuk et al, 2019), nematodes (John, 1967;Ryss & Polyanina, 2018), mycoplasmas (Bricker & Stutz, 2004), representatives of harmful entomofauna (Korda et al, 2019), as well as the influence of climatic and soil-hydrological indicators (Goberville et al, 2016), however, no consensus has been reached yet.…”

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confidence: 99%

Myco- and microorganisms in the tuberculosis pathology of the common ash in Ukraine and interaction between them

Kulbanska¹,

Goychuk²,

Shvets³

2021

Ukr. j. for. wood sci.

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Attention is focused on the fact that in recent years there has been epiphytotic dieback out of many species of forest woody plants both in Ukraine and in other countries of the world, which has a dynamic character and a tendency to grow. In the deep pathology of this phenomenon, phytopathogenic bacteria, which have high reproduction energy and can penetrate the plant both from the outside and cause a pathological process as vital obligates, were left without attention. It has been established that the most common and harmful disease of common ash in Ukraine is tuberculosis. The causative agent of the disease is the phytopathogenic bacterium Pseudomonas syringae pv. savastanoi Gard. affects both trunks, branches and shoots, and inflorescences of common ash. Bacteria Pseudomonas sp., Pseudomonas fluorescens Mig., Pseudomonas syringae Van., Erwinia herbicola Eh., Xanthomonas sp. were isolated from tuberculous pathology as a concomitant myco- and microbiota and micromycetes Cladosporium cladosporiodes Fres., Ulocladium botrytis Preus., Mycelia sterilia (dark), Mycelia sterilia (orange), Fusarium heterosporum Lin., Fusarium sp., W., Cylindrocarpon didymium Har., etc. The mechanism of systemic relationships of the components of myco- and microbiota of tuberculous pathology of common ash in the regulation (self-regulation) of pathogenicity and aggressiveness of vital obligates has been investigated. Attention is focused on the prospects and expediency of using the antagonistic properties of myco- and microorganisms and biological products based on them for the prevention and protection of tree plantations from bacterial pathogens. It is shown that the pathology of common ash is a multifaceted phenomenon with interrelated processes of an infectious and non-infectious nature. The need to distinguish between the etiology and pathogenesis of this negative phenomenon is indicated, that is, not to mix the factors that lead to the weakening of ordinary ash (factors catalyzing the disease) and the factors that cause its epiphytotic dieback. Keywords: bacteria, fungi, common ash, antagonism, Pseudomonas syringae pv. savastanoi.

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Tuberculosis Pathology of Fraxinus Excelsior L. in Ukraine: Symptomatology, Etiology, Pathogenesis

Goychuk

Kulbanska

Shvets³

2021

Scientific Horizons

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Given the forestry, the ecological and economic value of forests with the participation of Fraxinus excelsior and taking into account the intensive deterioration of their phytosanitary status in modern conditions, a comprehensive study of symptoms, causes, and pathogenesis of ash tuberculosis with further development of effective protection measures, including using biological products based on Bacillus sp. and other myco- and microorganisms, is a particularly relevant area of research. The aim of the research is to identify negative abiotic and biotic factors in the pathology of F. excelsior. In the process of research general scientific and special research methods were used (microbiological, mycological, phytopathological, entomological, and silvicultural-ecological methods). It is emphasized that the pathology of the common ash is a multifaceted phenomenon with interrelated processes of infectious and non-infectious nature, which in recent years has led to epiphytic dieback and now has a tendency to increase. It has been established that tuberculosis of F. excelsior is the most common and harmful disease within the study region and causes more economic than environmental damage. Bacteria of the genera Pseudomonas sp., Erwinia sp., and Xanthomonas sp., as well as micromycetes, which mainly take the place of the concomitant mycobiota. There are five stages (phases) of the development of tuberculosis pathology, which differ significantly in symptoms. The species composition of harmful insects has been identified. It is shown that hydrothermal stress is a catalyst for the epiphytic dieback of common ash. Direct dependence of the spread of tuberculosis on the share of ash in the stands of different age groups was revealed. In the study area, tuberculosis reaches epiphytotics on F. excelsior, especially at a young age. The expediency of distinguishing the causes and pathological processes associated with ash tuberculosis is indicated, so as not to confuse the disease-catalyzing factors and the factors that lead to the complete degradation of ash stands

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Phytopathogenic Bacteria in the Pathology of Forest Trees of Polyssya and Forest-Steppe of Ukraine

Cited by 3 publications

References 0 publications

Detection of Phytopathogenic Bacteria Damaging Weeping Birch (Betula Pendula) by Molecular Identification Method

Detection of Phytopathogenic Bacteria Damaging Weeping Birch (Betula Pendula) by Molecular Identification Method

Myco- and microorganisms in the tuberculosis pathology of the common ash in Ukraine and interaction between them

Tuberculosis Pathology of Fraxinus Excelsior L. in Ukraine: Symptomatology, Etiology, Pathogenesis

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