Phytochemistry, pharmacology and perceived health uses of non-cultivated vegetable Cyphostemma adenocaule (Steud. ex A. Rich.) Desc. ex Wild and R.B. Drumm: A review

Bello, Oluwasesan M.; Jagaba, Safiya Muhammad; Bello, Oluwatoyin E.; Ogbesejana, Abiodun B.; Dada, Adewumi O.; Adetunji, Charles Oluwaseun; Abubakar, Saudat Muhammad

doi:10.1016/j.sciaf.2019.e00053

Cited by 19 publications

(16 citation statements)

References 31 publications

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“…is a wild climbing plant widespread in West Africa countries, from Senegal east to Eritrea and south to Angola, Malawi and Mozambique (Bukill, 1985). Bello and colleagues gave a review on the plant together with its ethnomedicinal, phytochemistry and pharmacological properties (Bello et al, 2019). In this study, we report the isolation and characterization of a natural triglyceride; Trilinolein from the methanol root extract of C. adenocaule using standard chromatographic and spectroscopic technique.…”

Section: Introductionmentioning

confidence: 94%

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A Natural Triglyceride from the Methanol Root Extract of Cyphostemma Adenocaule (Steud. Ex A. Rich.) Wild & R.B.Drumm

Yakubu¹,

Iliya²,

bababe³

et al. 2020

Preprint

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Cyphostemma adenocaule (Steud. ex A. Rich.) is one of the specie plant that belongs to the family vitacea. In this study, Trilinolein was isolated and characterized from the methanol root extract of the plant. Column chromatography over silica gel granules as the stationary phase and eluted with a mobile phase mixture of n-Hex-EtA; EtA-CHCL3 and CHCL3-MeOH with gradient increasing polarity, followed by a second column using saphadex-LH20 and 100% MeOH as stationary and mobile phase vehicle respectively. TLC was developed with EtA 15: CHCL3 8: MeOH 4: H2O 1 as solvent system; sprayed with 10% H2SO4 ,Vanillin-sulphuric acid, and/ or Polyethylene glycol PEG and heat for spot detection and confirmation of bioactive principles. Compound CA1 was obtained and purified with CHCL3 to give a yellow semi-solid compound (0.23g). The 1H-NMR spectra showed 9 different signals; a signal peak of a glycerol (-CH2OCOR-) moiety on the first α-C chain and on the third αʹ-C at 4.143-4.187ppm and 4.296-4.325ppm respectively, while that of a β glycerol (-CHCOR-) at 5.286ppm. Signals of an allylic methylene group at 2.023-2.035ppm, Olefenic hydrogen group at signal peak of 5.362ppm and a diallylic methylene group at signal 2.790ppm were also observed. In the 13C NMR spectra of compound CA1, 57 carbon atoms where observed, multiple signals overlapping at a range of 14.13-34.21ppm corresponding to the aliphatic CH3 (C18), CH2 (C2, C3, C4, C5, C6, C7, C15, C16, and C17) and allylic (C8, C14) carbon atoms. Signals at 127.90-130.24ppm were assigned to the olefienic C atoms (C9, C10, C12, and C13) while signal of 172.87ppm and 173.32ppm were assigned to the carbonyl (C=O) carbon atoms (C1 and C2) respectively (Table 2). Analysis with DEPT-135, H-H COSY, HMBC and HSQC assignments of CA1 augments assignment of signals made for CA1 from 1H-NMR and 13C-NMR and corresponded to that of Trilinolein (<a href="https://pubchem.ncbi.nlm.nih.gov/#query=C57H98O6">C57H98O6</a>, MW 879.4 g/mol). The isolated compound was positive for the acrolein test for triglycerides; fat & oil and had an IC50 of 46.08µg/ml radical scavenging activity.

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Section: Introductionmentioning

confidence: 94%

“…In this study, we report the isolation and characterization of a natural triglyceride; Trilinolein from the methanol root extract of C. adenocaule using standard chromatographic and spectroscopic technique. (Bello et al, 2019)…”

Section: Introductionmentioning

confidence: 99%

A Natural Triglyceride from the Methanol Root Extract of Cyphostemma Adenocaule (Steud. Ex A. Rich.) Wild & R.B.Drumm

Yakubu¹,

Iliya²,

bababe³

et al. 2020

Preprint

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“…In traditional medicine, C. adenocaule has been used for the treatment of various illnesses such as malaria, helminthic infection, cancer, tumors, snake bite, rabies, and anthrax [ 3 – 6 ]. Earlier scientific studies showed that C. adenocaule has in vitro antiplasmodial [ 7 ], antioxidant [ 8 ], and deworming [ 9 ] effects. The anti-infective, antitoxin, and antitumor activity of this plant might be due to its effect on the cellular immune system and accessory cell types.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the Effect of Hydroethanolic Root Extract and Solvent Fractions of Cyphostemma adenocaule (Steud. ex A. Rich) Descoings ex Wild & Drummond on Cell-Mediated Immune Response and Blood Cell Count in Mice

Abebe

Periasamy

Karim

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Introduction. Cyphostemma adenocaule (Steud. ex A. Rich) Descoings ex wild & Drummond (Vitaceae) has been used in traditional medicine for the management of various immunological and hematological disorders in different areas of Ethiopia and the rest of the world. In Ethiopia, the plant is used for the management of enlarged spleen, rabies virus, helminthic infection, snake bite, and various types of tumors. Objective. To evaluate the effect of hydroethanolic root extract and solvent fractions of Cyphostemma adenocaule on cell-mediated immunity (delayed-type hypersensitivity), organ index (spleen and liver), and blood cell count in Swiss albino mice. Materials and Methods. Acute oral toxicity test was conducted using nulliparous and nonpregnant Swiss albino mice following OECD 425 limit test method. Delayed-type hypersensitivity model was used to evaluate the effect on cell-mediated immunity. The experimental animals were divided into twelve groups which were sensitized and challenged with sheep red blood cells on day 0 and day 7, respectively. Levamisole 50 mg/kg was used as stimulant control, whereas cyclophosphamide 30 mg/kg was used as suppressant control. Hydroethanolic root extract (100 mg/kg, 200 mg/kg, and 400 mg/kg), aqueous fraction (100 mg/kg, 200 mg/kg, and 400 mg/kg), and n-butanol fraction (100 mg/kg, 200 mg/kg, and 400 mg/kg) were administered for seven days. The paw volume was measured using a digital plethysmometer before challenge and 24 hours after challenge. Blood was collected, and organs (spleen and liver) were isolated from each challenged mouse to determine blood cell count and organ index, respectively. Results. No mortality and noticeable behavioral changes were observed among all mice receiving hydroethanolic root extract and solvent fractions at a dose of 2000 mg/kg. Hydroethanolic root extract and solvent fractions of Cyphostemma adenocaule showed enhancement of delayed-type hypersensitivity, organ index, and blood cell count. Hydroethanolic root extract at a dose of 400 mg/kg showed the highest and statistically significant stimulation of delayed-type hypersensitivity (0.123 ± 0.010) and blood cell count compared to the vehicle. Conclusion. Hydroethanolic root extract and solvent fractions of Cyphostemma adenocaule showed a stimulatory effect on cell-mediated immunity and hematopoiesis.

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“…Rich.) Wild & R.B.Drumm is a climbing, scrambling or trailing herb that belongs to the Vitaceae family (Burkill, 1985 andBello et al, 2019) and locally known as yáákùwár fátààkéé (Hausa, Nigeria) (Burkill, 1985). The plant is a popular, noncultivated vegetable eaten in many parts of Africa i.e.…”

Section: Introductionmentioning

confidence: 99%

“…The plant is a popular, noncultivated vegetable eaten in many parts of Africa i.e. Nigeria, Ghana, Congo, Uganda, Ethiopia and Eritrea (Bello et al, 2019). The plant had been documented for its ethnomedicinal value, with a comprehensive review given by Bello et al (2019).…”

Section: Introductionmentioning

confidence: 99%

Phytochemical Screening, Antioxidant and Antibacterial Activity of the Root Extract of Cyphostemma Adenocaule (Steud. Ex A. Rich.) Wild & R.B.Drumm

Yakubu¹,

Mohammed²,

bababe³

et al. 2020

Preprint

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ABSTRACTPlant secondary metabolites had provided important bioactive principles for developing new lead compounds. Within their confinement, they exhibit unique chemical diversity, which influence their diverse biological properties. The vitaceae family are known for their potent antioxidant and antibacterial phytoconstituents, among other biological properties. Cyphostemma adenocaule is one of the family members explored for its ethnomedicinal properties. This study undertook the evaluation of the phytochemical, antioxidant and antibacterial properties of the root extract of Cyphostemma adenocaule, as well as, evaluating their Biological properties; antioxidant activity (DPPH assay) and antibacterial activity (agar well diffusion test). Preliminary phytochemical screening revealed the presence of flavonoids, alkaloids, carbohydrates & glycoside, alkaloids, saponins and tannins. The methanol root extract had the highest percentage in the DPPH assay, providing 50% inhibition IC50 of 10.87µg/ml, followed by nHexane (IC50 74.10µg/ml) and chloroform (IC50 74.31µg/ml) extract. In the antibacterial assay, the chloroform extract was active against E. coli (24.00±0.15) and moderate activity against Staph. aureus (12.5±0.18). The nHex extract was completely inactive against the test organism while the methanol extract showed poor activity against the test organism. The present study adds to the existing literature on Cyphostemma adenocaule with scientific evidence into its biological properties. Keywords: Cyphostemma adenocaule, Phytochemical screening, antioxidant and antibacterial activity

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Phytochemistry, pharmacology and perceived health uses of non-cultivated vegetable Cyphostemma adenocaule (Steud. ex A. Rich.) Desc. ex Wild and R.B. Drumm: A review

Cited by 19 publications

References 31 publications

A Natural Triglyceride from the Methanol Root Extract of Cyphostemma Adenocaule (Steud. Ex A. Rich.) Wild & R.B.Drumm

A Natural Triglyceride from the Methanol Root Extract of Cyphostemma Adenocaule (Steud. Ex A. Rich.) Wild & R.B.Drumm

Evaluation of the Effect of Hydroethanolic Root Extract and Solvent Fractions of Cyphostemma adenocaule (Steud. ex A. Rich) Descoings ex Wild & Drummond on Cell-Mediated Immune Response and Blood Cell Count in Mice

Phytochemical Screening, Antioxidant and Antibacterial Activity of the Root Extract of Cyphostemma Adenocaule (Steud. Ex A. Rich.) Wild & R.B.Drumm

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Phytochemistry, pharmacology and perceived health uses of non-cultivated vegetable Cyphostemma adenocaule (Steud. ex A. Rich.) Desc. ex Wild and R.B. Drumm: A review

Cited by 19 publications

References 31 publications

A Natural Triglyceride from the Methanol Root Extract of Cyphostemma Adenocaule (Steud. Ex A. Rich.) Wild &amp; R.B.Drumm

A Natural Triglyceride from the Methanol Root Extract of Cyphostemma Adenocaule (Steud. Ex A. Rich.) Wild &amp; R.B.Drumm

Evaluation of the Effect of Hydroethanolic Root Extract and Solvent Fractions of Cyphostemma adenocaule (Steud. ex A. Rich) Descoings ex Wild & Drummond on Cell-Mediated Immune Response and Blood Cell Count in Mice

Phytochemical Screening, Antioxidant and Antibacterial Activity of the Root Extract of Cyphostemma Adenocaule (Steud. Ex A. Rich.) Wild &amp; R.B.Drumm

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A Natural Triglyceride from the Methanol Root Extract of Cyphostemma Adenocaule (Steud. Ex A. Rich.) Wild & R.B.Drumm

A Natural Triglyceride from the Methanol Root Extract of Cyphostemma Adenocaule (Steud. Ex A. Rich.) Wild & R.B.Drumm

Phytochemical Screening, Antioxidant and Antibacterial Activity of the Root Extract of Cyphostemma Adenocaule (Steud. Ex A. Rich.) Wild & R.B.Drumm