Phytochemicals Screening and Antioxidant Effectiveness of Garlic (Allium sativum) from Timor Island

Priska, Melania; Peni, Natalia; Carvallo, Ludovicus

doi:10.15294/biosaintifika.v11i1.17313

Cited by 8 publications

(10 citation statements)

References 11 publications

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“…For the Dragendorff, the formation of an orange or orange-red precipitate testified to the presence of alkaloids. In contrast, for the Wagner reagent, the shape of a brownish-black precipitate indicated the presence of alkaloids [14,15].…”

Section: Alkaloidsmentioning

confidence: 87%

Preliminary phytochemical and hepatoprotective studies of ethanol extract of Desmodium repandum (Vahl) D.C. in paracetamol induced liver injury in guinea pig

Safari¹,

Mutelesi²,

Bapolisi³

et al. 2022

GSC Biol. Pharm. Sci.

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Medicinal plants are used worldwide in folk medicine to treat many diseases, including those affecting the liver, but most of those plants are not yet studied. The present study aimed at the in vivo evaluation of the hepatoprotective potential and doing a preliminary phytochemical screening of Desmodium repandum (Vahl) D.C. leaves ethanolic extract, a plant used in many regions of Africa to treat liver diseases. The presence of main phytochemical groups was assessed by using specific reactions of identification. Hepatoprotective activity was evaluated in male guinea pigs; the hepatotoxicity was induced by oral administration of paracetamol (acetaminophen) at the dose of 500 mg/kg. Silymarin was used as the reference drug at the dose of 100 mg/kg, the control group received a solution of carboxymethylcellulose (CMC) 1%, and the experimental groups 100 and 200 mg of the extract per kg of body weight. The results of phytochemical screening revealed the presence of anthocyanin, phenolic compounds, coumarins, flavonoids, quinones, tannins, sterols, and triterpenes. At the dose of 200 mg/kg, the extract reduced significantly (p ˂ 0.05) the blood concentration of aspartate transaminase (AST), alanine transaminase (ALT), and total bilirubin after intoxication by paracetamol comparatively to the control group. This work demonstrated for the first time the hepatoprotective potential of the ethanolic extract of Desmodium repandum (Vahl) D.C. leaves.

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Section: Alkaloidsmentioning

confidence: 87%

Preliminary phytochemical and hepatoprotective studies of ethanol extract of Desmodium repandum (Vahl) D.C. in paracetamol induced liver injury in guinea pig

Safari¹,

Mutelesi²,

Bapolisi³

et al. 2022

GSC Biol. Pharm. Sci.

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“…1C). Based on the literature ECx has low antioxidant activity because the IC50 value is more than 150 ppm (Priska et al, 2019). However, the anticancer activity is quite strong, with an IC50 value of less than 100 µg/mL.…”

Section: Cell Viability Phytochemical Screening and Antioxidant Activitymentioning

confidence: 99%

Potential of Curcuma xanthorrhiza ethanol extract in inhibiting the growth of T47D breast cancer cell line: In vitro and bioinformatic approach

Fitriana¹,

Rifa’i²,

Masruri³

et al. 2022

J Pharm Pharmacogn Res

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Context: Breast cancer most commonly occurs in women globally and has the highest mortality rate in Asia. Therefore, a safe and prominent drug to cure the disease needs to be urgently developed. Aims: To investigate the molecular mechanism of the ethanol extract of Curcuma xanthorrhiza (ECx) in inducing apoptosis in breast cancer cell line T47D. Methods: The research was started by extracting Curcuma xanthorrhiza using ethanol as solvent. The anticancer research was carried out by cell toxicity assay and apoptosis assay. This study also observed changes in cell morphology and protein expression levels that can induce cell apoptosis. The bioinformatics approach was carried out to determine the activity of the active compound in inhibiting AKT-1, which plays an important role in the development of cancer cells. TIG-1 cells were used as controls in toxicity assays. Results: ECx showed antioxidant and nitric oxide scavenging activity, which is beneficial for human health, and exhibited selective toxicity in T47D breast cancer cells compared to TIG-1 normal cells. ECx increased the expression of p53, Bax, caspase-3, and caspase-9, which induces apoptosis. Further analysis showed that ECx contained at least eight active compounds: curcumin, curcumin II (desmethoxycurcumin), curcumene, camphor, 1,8-cineole, p-cymene, ar-turmerone, and caryophyllene oxide. Bioinformatics studies suggest that active compounds may be involved in apoptosis via the PI3K/AKT signaling pathway. Conclusions: ECx significantly acts as an anticancer agent by inhibiting the growth of T47D cells. This research proves that the bioinformatics approach shows that curcumin can inhibit the expression of AKT-1.

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“…This plant contains sulphuric compounds, proteins and minerals, also allicin which has the ability as antimicrobials [14,36]. Other secondary metabolites, including flavonoids, phenols, terpenoids, and alkaloids are also present in this species [37]. Indonesian people consume this plant by directly chew on them or employ them as cooking material [18].…”

Section: Ethnomedicinal Research Across Indonesiamentioning

confidence: 99%

Upgrading Indonesian Local Ethnomedicinal Knowledge with Molecular Phylogenetics

Brazia¹,

Adi²,

Kaho³

et al. 2020

Proceedings of the 5th International Conference on Food, Agriculture and Natural Resources (FANRes 2019)

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With at least 200 ethnic groups and 30.000 medicinal plants used in Jamu formulations, Indonesia is one of the largest natural drug dispensaries in the world. Ethnomedicine-directed bioprospecting has been used to accelerate drug discovery. Little is known about the coverage of ethnomedicinal studies in Indonesia. The quest to obtain drugs from plants also remains elusive. Nature-derived approved drugs worldwide have come mostly from drug-productive families that tend to be clustered in the phylogenetic tree, thus these families should be the basis of bioprospecting focus. We presented a review of ethnomedicinal research conducted across Indonesia and recommend strategic approaches to accelerate the translation of traditional knowledge into the clinic. We identified several plant species commonly used with similar or different purposes within different places, empirical pharmacological studies, regions that are understudied, and comparison with plants whose metabolites have been used for the development of approved medicine worldwide. Combination of ethnomedicinal knowledge with molecular phylogenetics,-omics, Bioactivity-guided bioprospecting, DNA barcoding, and Global Biodiversity Information Facility (GBIF) software will enable more efficient and environmentally friendly drug discovery process as well as commercial production. Altogether, this proposed method will be a game-changer in the exploration of the largely untapped chemical diversity of Indonesian flora.

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Phytochemicals Screening and Antioxidant Effectiveness of Garlic (Allium sativum) from Timor Island

Cited by 8 publications

References 11 publications

Preliminary phytochemical and hepatoprotective studies of ethanol extract of Desmodium repandum (Vahl) D.C. in paracetamol induced liver injury in guinea pig

Preliminary phytochemical and hepatoprotective studies of ethanol extract of Desmodium repandum (Vahl) D.C. in paracetamol induced liver injury in guinea pig

Potential of Curcuma xanthorrhiza ethanol extract in inhibiting the growth of T47D breast cancer cell line: In vitro and bioinformatic approach

Upgrading Indonesian Local Ethnomedicinal Knowledge with Molecular Phylogenetics

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