Phytochemical Screening by FTIR Spectroscopic Analysis and Antibacterial Activity of Sesbania grandiflora

Ndahawali, Suryaningsih; Andayani, Sri; Hardoko, Hardoko

doi:10.21776/ub.jels.2019.009.03.06

Cited by 1 publication

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“…The shape of the spectrum was examined, and unique peaks suggesting functional groups possessed by a compound were identified [12]. The absorption bands developed during FTIR identification of S. Grandiflora revealed that the n-hexane fraction contained flavonoids and terpenoids [13]. Investigating in vitro antioxidants and FTIR analysis of Mannar bay seaweed.…”

Section: Introductionmentioning

confidence: 99%

In Vitro and FTIR Spectroscopy: Local Black Fruit Seed Extract as Antibacterial Aeromonas Hydrophila

Toja,

Suprayitno,

et al. 2021

International Journal of Biology and Biomedical Engineering

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Indonesia has many local plants with potential as herbal antibacterial properties, one of which is the local black fruit of Wandama. The objective of this study is to determine the antibacterial activity of the extract of black fruit seed against A. Hydrophila by in vitro and FTIR by Tilapia. The benefit of this research is to make black fruit seed extract as a natural remedy against tilapia attacked by Aeromonas Hydrophila. The antibacterial activity of black fruit seed extract against A. Hydrophila is investigated in vitro and using FTIR on tilapia in this research. The aim of this study is to establish black fruit seed extract as a natural treatment for tilapia infected with Aeromonas Hydrophila. The disc approach was used to conduct the in vitro research. Agar medium was poured into a sterile petri dish, and 2 drops of bacteria from the liquid medium were uniformly distributed and cooled. On the press, disc paper with black fruit seed extract was put and incubated for 18-24 hours at 35 C. An FTS 1000 version spectrophotometer system was used for the FTIR test. For the FT-IR study, a fraction of dried black fruit seeds was used. For clear preparation, 100 mg of dry extract is condensed in KBr pellets. A specimen of loaded black fruit seed samples was analyzed using FT-IR spectroscopy with a scanning range of 400-4000 cm-1 and a resolution of 4 cm-1. The results showed that the ethyl acetate fraction produced an inhibition zone (13.65 mm), the water fraction produced an inhibition zone (11.21 mm), and the n-hexane fraction produced the weakest inhibition zone (11.21 mm) (5.31mm). The concentration test results from 125 ppm, 250 ppm, 500 ppm and 1000 ppm obtained a large inhibition zone at a concentration of 1000 ppm with an inhibition zone area of 11.43 + 0.02. The absorbance value of black fruit seed extract in ethyl acetate solvent at 3430 cm-1 revealed the absorption band with the strongest vibration of the hydroxylate (-OH) functional group, according to FTIR research. Final thoughts with an average value of 13.65 + 0.06, black fruit seed extract using ethyl acetate can inhibit A. Hydrophila bacteria.

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Section: Introductionmentioning

confidence: 99%