Phytochemical screening, antibacterial and antioxidant assessment of Leuconotis eugenifolia leaf extract

Praptiwi,; Wulansari, Dewi; Fathoni, Ahmad; Harnoto, Noto; Novita, Rossi; Alfridsyah,; Agusta, Andria

doi:10.13057/nusbiosci/n120114

Cited by 6 publications

(9 citation statements)

References 23 publications

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“…The crude extract was kept at 40°C until it was analyzed. Phytochemical analysis 35,36 Phytochemical compounds were identified by qualitative analysis. The extract's content of flavonoids, alkaloids, saponins, tannins, steroids, and terpenoids was determined qualitatively.…”

Section: Taro (Colosia Esculenta) Leaves Extract Inhibitsmentioning

confidence: 99%

Taro (Colosia esculenta) Leaves Extract Inhibits Streptococcus mutans ATCC 31987

et al. 2022

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Dental caries was the most common disease in both adults and children. Streptococcus mutans is the main bacteria causing plaque formation and was the initiator of dental caries. Antibacterials derived from plants can be used to prevent plaque formation. Taro (Colosia esculenta) has been used in traditional medicine. Antibacterial compounds have been discovered in C. esculenta leaves. This study aimed to determine the ability of C. esculenta leaf ethanol extract to inhibit the growth of S. mutans ATCC 31987. Simplicia preparation, extract preparation, and phytochemical screening was carried out. Then, the antibacterial activity test was performed using the disc diffusion method to determine the zone of inhibition at various concentrations of 10, 20, 30, 40, 50, 60, and 70%. Colosia esculenta leaf ethanol extract contains alkaloids, flavonoids, triterpenoids, saponins, and produces an inhibition zone at each concentration variation. Very strong antibacterial activity was produced at a concentration of 70% at 21.11±0.46 mm, which was higher than the positive control.

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Section: Taro (Colosia Esculenta) Leaves Extract Inhibitsmentioning

confidence: 99%

Taro (Colosia esculenta) Leaves Extract Inhibits Streptococcus mutans ATCC 31987

et al. 2022

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“…15 mg of each extract was mixed separately on a water bath with 6 ml of 1% HCl and filtered. These filtrates were then tested with Dragendorff's test, Mayer's test, and Wagner's test, respectively [9].…”

Section: Alkaloidsmentioning

confidence: 99%

“…Then, added 2 mL of 10% AlCl3 and 1 M NaOH into the solution. The presence of flavonoid was indicated by the yellow color formation of the solution [9].…”

Section: Flavonoidsmentioning

confidence: 99%

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The Preliminary Study of Antimicrobial Activity of Borassus flabelifer L. Mesocarp Extract

Mahayasih

Mahayasa

2022

JPRI

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Lontar or Borassus flabelifer is a local plant that mostly found in Nusa Tenggara Province of Indonesia. The juice of the ripe mesocarp has been used traditionally for treatment minor oral ulcer. Microorganism is one of the factors that cause the recurrence of oral ulcer. This study was aimed to investigate the antimicrobial potency of the B. flabelifer fresh and dried mesocarp extract. The antimicrobial extract was tested in vitro by using the agar well diffusion method against four bacteria, Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and one fungus, Candida albicans with concentration of the extract were 10 mg/mL and 100 mg/mL. The dried and fresh mesocarp extract was resistant to P. aeruginosa and E. coli, while the dried mesocarp extract showed potential antibacterial activity against B. subtilis and S. aureus with the diameter zone of inhibition on concentration 10 mg/ml were 12 ± 0.1 mm and 10± 0.1 mm, respectively. Antifungal activity only exhibited by the dried mesocarp extract with diameter zone of inhibition on concentration 100 mg/mL was 9 ± 0.1 mm. The dried mesocarp extract was more potent than fresh mesocarp extract as antimicrobial.

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“…Subsequently, 10 µl of filtrate was placed on the thin layer chromatography (TLC) plate of silica F 254, and vitamin C solution was used as a positive control. The F 254 TLC plate was inserted into the chamber containing the mobile phase of chloroform, methanol, and distilled water (6:4:1) (Praptiwi et al 2020). The TLC plate was sprayed with 0.002% DPPH solution, and the observed spots were used to calculate the retardation factor (Rf) with the following formula:…”

Section: Antioxidant Activity Testing Using Thin Layer Chromatographymentioning

confidence: 99%

Potential of Endophytic Fungi Deriving From Asiatic Pennyworth to Produce Antioxidants

Susilowati¹,

Radiastuti

Nurhasni

et al. 2021

Indones. J. Agric. Sci.

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Asiatic pennyworth is a medicinal plant that contains triterpenoids, saponin, flavonoids, and tannins which possess antioxidants. Endophytic fungi from the plant could produce a similar compound; therefore, antioxidants could be made in the laboratory if the fungi are isolated. This study aimed to evaluate the potential of endophytic fungi isolated from Asiatic pennyworth to produce antioxidants. The study used 34 endophytic fungal isolates from Asiatic pennyworth accessions of Malaysia (17 isolates) and Bengkulu, Indonesia (17 isolates) collected by the Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development. The fungi were propagated in a potato dextrose broth medium, then mycelia mats and filtrates were separated and then freeze-dried. The antioxidant activities were first tested with 1,1-diphenyl-2- picrylhydrazyl (DPPH) using thin layer chromatography (TLC), then UV-Vis spectrophotometry λ517 nm with five variations of concentration. Results showed all 34 fungal isolates have antioxidant activities based on a yellowish-white color change after applying 0.002% DPPH solution of the TLC method and IC50 value of the UV-Vis spectrophotometer. The highest antioxidant activity was shown by Aspergillus austroafricanus MB 1 (IC50 = 12.08 ppm) from Bengkulu accession and A. oryzae MM 13 (IC50 = 10.29 ppm) from Malaysia accession. A. austroafricanus MB 1 produced more antioxidant compounds (seven) than A. oryzae MM 13 (six). The antioxidant compounds produced by both endophytic fungi included in the group of flavonoids, fatty acids, and carboxylic acids. The research implies that A. austroafricanus MB 1 and A. oryzae MM 13 could be further developed as sources of antioxidants.

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Phytochemical screening, antibacterial and antioxidant assessment of Leuconotis eugenifolia leaf extract

Cited by 6 publications

References 23 publications

Taro (Colosia esculenta) Leaves Extract Inhibits Streptococcus mutans ATCC 31987

Taro (Colosia esculenta) Leaves Extract Inhibits Streptococcus mutans ATCC 31987

The Preliminary Study of Antimicrobial Activity of Borassus flabelifer L. Mesocarp Extract

Potential of Endophytic Fungi Deriving From Asiatic Pennyworth to Produce Antioxidants

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