Phytochemical Screening and Thin Layer Chromatography of Indian Asparagus Officinalis Linn.

Begum, A. N.; Rao, K. Ramesh; Dutt, Rajeswar; Giri, Krishna; Sindhu, K. K.; Umera, Fathima; Gowthami, G; Kumar, J Ravi; N, Naveen Kumar; Shaffath., Syed

doi:10.21474/ijar01/3976

Cited by 5 publications

(3 citation statements)

References 3 publications

(5 reference statements)

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“…were the phytoconstituents found in plants. The result of phytochemical analysis are consistent with previous studies that have screened for phytochemicals in Asparagus racemosus (Begum et al, 2017), Bergenia ciliate (Bhandari et al, 2019), Withania somnifera (Arya…”

Section: Phytochemical Screeningsupporting

confidence: 90%

Formulation of Herbal Tea from Nepalese Medicinal Plants: Phenolic Assay, Proximate Composition and In-vivo Toxicity Profiling of Medicinal Plants with Nutritive Benefits

Mandal¹,

Pandey

Pant

et al. 2022

J.Pl.Reso.

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Herbal tea, also known as tisane, is a beverage made from the infusion or decoction of plant material in hot water. True tea comes from the Camellia sinensis plant, while tisane (herbal tea) comes from a water-based infusion of herbs, spices, flowers, leaves etc. This study aimed to formulate and determine the nutraceutical value (proximate analysis), phytochemical value (total phenolic content) and in-vivo toxicity of the different medicinal plants used to prepare three different types of herbal tea formulations. Natural Product Research Laboratory (NPRL)-1 [Asparagus officinalis L., Phyllanthus emblica L., Mentha piperita L., Elettaria cardamomum (L.) Maton and Camellia sinensis (L.) Kuntze], NPRL-2 [Ocimum tenuiflorum L., Bergenia ciliate (Haw.) Sternb., Elettaria cardamom (L.) maton and Camellia sinensis (L.) Kuntze] and NPRL-3 [Salvia rosmarinus Spenn., Cymbopogon citratus (DC.) Stapf, Senegalia catechu (L.f.) P.J.H.Hurter & Mabb. Elettaria cardamomum (L.) Maton and Withania somnifera (L.) Dunal] herbal tea formulations were prepared from these selected medicinal plants. They were respectively tested for their properties. All the plants included were highly nutritional and none were found toxic. The results suggested that herbal tea made up of these potent plants’ parts can be a good choice for health-promoting benefits. These formulations could further be studied for their other beneficial activities.

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Section: Phytochemical Screeningsupporting

confidence: 90%

Formulation of Herbal Tea from Nepalese Medicinal Plants: Phenolic Assay, Proximate Composition and In-vivo Toxicity Profiling of Medicinal Plants with Nutritive Benefits

Mandal¹,

Pandey

Pant

et al. 2022

J.Pl.Reso.

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“…Alkaloids are indicated by the presence of red-orange or yellowish-orange spots on a yellow background with the detection of Dragendroff's reagent (Wagner & Bladt, 1996). According to Begum et al (2017), the results of phytochemical screening of a compound may vary depending on the eluent used during the TLC test.…”

Section: Resultsmentioning

confidence: 99%

Determination of Streptomyces sp. SAE4034 Antioxidant and Antibacterial Compound and Its Inhibitory Mechanism on Staphylococcus aureus

Kurniawati

Ryandini

Hernayanti

2023

bioe

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The genus Streptomyces is known as producers of bioactive compounds that have antibacterial and antioxidant properties. Streptomyces sp. SAE4034 was known to produce antibacterial compounds that can inhibit and kill pathogenic bacteria, including Staphylococcus aureus, but the group of antibacterial compounds and their inhibitory mechanisms were not yet known. The ability of Streptomyces sp. SAE4034 in producing antioxidants is unknown yet. This research aimed to determine the classification of antibacterial compounds produced by Streptomyces sp. SAE4034, to know the ability of Streptomyces sp. SAE4034 in producing antioxidant compounds, and to observe the inhibitory mechanisms of antibacterial compounds produced by Streptomyces sp. SAE4034 against S. aureus using cell leakage method. The results of this research show that Streptomyces sp. SAE4034 produce compounds in the group of alkaloid, terpenoid, flavonoid, polyphenol. Compound with Rf values 0,40 and 0,47 which includes into polyphenol and 0,72 which includes into alkaloid group has the ability to inhibit the growth of S. aureus. Streptomyces sp. SAE4034 has a very strong antioxidant activity that can scavenge DPPH free radical with IC50 value of 32,14 ppm and has an antioxidant enzyme activity that is superoxide dismutase amount of 63,10 U/mL The inhibitory mechanism of Streptomyces sp. SAE4034 extract is through inhibition of protein membrane synthesis by the release of nucleic acid and protein material in S. aureus cells and the highest cell leakage was at 2 MIC doses.

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“…A small portion of the total extract of L. kituiensis Vatke was reconstituted in MeOH: Dichloromethane mixture and spotted on cut pieces of pre-coated thin layer chromatography plates (TLC). The plates were then developed in different solvent pairs (Begum et al, 2017). Upon development, assessment of plates was done by observation on open light, under UV (254 and 365 nm), and after vanillin spray with plates heated to 110°C for 5 min.…”

Section: Thin Layer Chromatographymentioning

confidence: 99%

Antimalarial potential and phytochemical composition of fractions of Lippia kituiensis Vatke (Verbenaceae) growing in Northern Tanzania

Ngâ€TMetich¹,

Swai²,

Njokah³

et al. 2020

J. Med. Plants Res.

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Despite past decades of steady advances in the fight against Malaria, statistics show that the disease is still a threat to human health. Previous successes in the development of antimalarial drugs from medicinal plants have fuelled research in this area. However, antimalarial studies on fractionated extracts from such plants have progressed slowly. This study reports the antimalarial potential of fractions from Lippia kituiensis Vatke, for the first time. Column chromatography was used during fractionation. Antiplasmodial assay against chloroquine-sensitive (D6) and resistant (W2) plasmodium strains were done using hypoxanthine incorporation assay. MTT assay was used to assess the cytotoxicity of fractions against the Vero cell line. Fractions obtained exhibited varied inhibitory concentrations (IC50); with the most efficacious being, Lk-5 (19.45 ± 6.20 μg/ml), Lk-3 (30.43 ± 0.68 μg/ml), Lk-4 (30.82 ± 18.01 μg/ml), and Lk-6 (36.53 ± 14.42 μg/ml) against D6. Generally, lower activity against W2 was obtained with the most active being LK-4 (24.18±2.50 μg/ml), and Lk-5 (24.42±5.95 μg/ml), while chloroquine (positive control) exerted IC50 of 77.86±4.09 ng/ml (W2) and 15.71±6.49 ng/ml (D6). LK-4 was the most cytotoxic showing cytotoxic concentration (CC50), 46.26 μg/ml. When tested in mice, fractions suppressed Plasmodium berghei significantly compared to the negative control with Lk-3 being most efficacious (80.01±1). Due to substantive efficacy, GC-MS done on Lk-3 revealed 8 compounds where three have previously been ascribed to antimalarial activity and other pharmacological effects. This study adds to present knowledge of antimalarial efficacy of L. kituiensis and provides the basis for further work to be done on the isolation of compounds from its extracts.

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Phytochemical Screening and Thin Layer Chromatography of Indian Asparagus Officinalis Linn.

Cited by 5 publications

References 3 publications

Formulation of Herbal Tea from Nepalese Medicinal Plants: Phenolic Assay, Proximate Composition and In-vivo Toxicity Profiling of Medicinal Plants with Nutritive Benefits

Formulation of Herbal Tea from Nepalese Medicinal Plants: Phenolic Assay, Proximate Composition and In-vivo Toxicity Profiling of Medicinal Plants with Nutritive Benefits

Determination of Streptomyces sp. SAE4034 Antioxidant and Antibacterial Compound and Its Inhibitory Mechanism on Staphylococcus aureus

Antimalarial potential and phytochemical composition of fractions of Lippia kituiensis Vatke (Verbenaceae) growing in Northern Tanzania

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