Phytochemical Screening and Preliminary TLC Characterization of Alkaloids in Sabicea brevies Root

Ogbuanu, C. C.; Ehiri, Richard C.; Ogah, S. P. I.

doi:10.3923/rjphyto.2014.1.8

Cited by 3 publications

(3 citation statements)

References 8 publications

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“…Using ferric Chloride test, a few drops of neutral Ferric chloride (FeCl₃) solution was added to 2 ml of each solvent extract solution in distilled water (1mg/ml), a blackish red color forms, indicating the presence of flavonoids. [30] The presence of phenols and tannins was evaluated using Ferric Chloride, where 2-3 drops of neutral (5%) Ferric chloride solution was slowly added along the wall of a test tube containing 1 ml of the filtrate (1mg/ml). A reddish-green-blue or violet coloration indicated the presence of a phenolic hydroxyl group/ phenols presence.…”

Section: Phytochemical Screening Of Extractsmentioning

confidence: 99%

“…A reddish-green-blue or violet coloration indicated the presence of a phenolic hydroxyl group/ phenols presence. [30,31] Tannins were also analyzed by Ferric chloride test using 2 ml of each solvent extract (50mg/ml) by adding 2-3 drops of 5% ferric chloride (FeCl 3 ). A greenish to black transient color was positive for tannins presence.…”

Section: Phytochemical Screening Of Extractsmentioning

confidence: 99%

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Rubia cordifolia L. Crude Aerial Part Extracts Show a High Potential as Antimalarials against Chloroquine Resistant Plasmodium falciparum Strains in vitro

Jeremiah¹,

Kamau²,

Nyambati³

et al. 2023

Pharmacogn. Res.

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Introduction: Plasmodium falciparum human malaria parasite is resistant to most available medicines in Africa, necessitating research for new drugs. Rubia cordifolia L. is used in traditional malaria treatment in Kenya despite no supporting scientific data. Objectives: To evaluate anti-plasmodial efficacy of extracts from the aerial part of Rubia cordifolia against chloroquine sensitive (3D7) and resistant (W2) Plasmodium falciparum strains. Methods: Hexane, methanol and water were separately used in crude extractions from open shade dried aerial plant parts. Phytochemical composition was determined qualitatively. Anti-plasmodial efficacy of serial dilutions (range; 0.4 to 50 μg/ml) of each extract were evaluated in vitro and compared using ANOVA and Tukey's HSD test, at p<0.05). Results: All the extracts significantly inhibited parasites growth, in a dose dependent manner. At 50 μg/ml, inhibition was (84.07%, 77.94% and 66.08%) for hexane, methanol and water extracts respectively against W2 and (80.06%, 78.36% and 60.92%) against 3D7 strain. Hexane extract showed IC 50 0.551 µg/ml and 2.747 micrograms/ml against W2 and 3D7 respectively. Methanol extract, IC 50 1.231 and 3.528 µ g/ml respectively, aqueous extract showed moderate activity, IC 50 5.348 μg/ml and 17.341 µg/ml against the strains respectively. Chloroquine resistant (W2 strain) was more responsive to the extracts than 3D7 strain. Conclusion: Anti-plasmodial activities were in the order, Hexane>methanol>aqueous and were attributed to the phytochemicals present in the extracts. This presents evidence of R. cordifolia as anti-malarial drugs' source. Further work is required to identify, purify, and characterize active molecules for development of anti-malaria drugs.

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Section: Phytochemical Screening Of Extractsmentioning

confidence: 99%

Section: Phytochemical Screening Of Extractsmentioning

confidence: 99%

Rubia cordifolia L. Crude Aerial Part Extracts Show a High Potential as Antimalarials against Chloroquine Resistant Plasmodium falciparum Strains in vitro

Jeremiah¹,

Kamau²,

Nyambati³

et al. 2023

Pharmacogn. Res.

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“…Metode KLT 31 . Nilai Rf senyawa fitokimia penelitian ini sesuai dengan kisaran nilai Rf pada pelarut kloroform dan etanol penelitian lain, yaitu flavonoid (0.5 -0.9); tanin (0.5 -0.9); (saponin (0.5 -0.8); alkaloid (0.6 -0.7); terpenoid (0.1 -0.9) 32,33 . Senyawa dengan nilai Rf yang lebih rendah memiliki koefisien distribusi yang lebih besar, karena senyawa tertahan lebih kuat pada fase diam daripada fase geraknya 34 .…”

Section: Tabel 1 Senyawa Metabolit Sekunder Pada Ekstrak Metanol Bounclassified

Studi Toksisitas: Ekstrak Metanol Bonggol Pisang Ambon (Musa acuminata L. cv. Gros Michel) terhadap Aedes aegypti (Diptera: Culcidae)

2018

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Aedes aegypti mosquito is the main vector of dengue virus causing dengue hemorrhagic fever (DHF) in Indonesia. The main preventive action is to control the presence of Ae. aegypti mosquito. Banana plants are known to contain secondary metabolite compounds acting as a natural insecticide, including the hump part. This research was conducted to evaluate to toxicity of hump of Ambon banana extract (Musa acuminata L. cv. Gros Michel) to dengue vector Ae. aegypti mosquito. The research used experimental method, the treatment concentration was 0.001; 0.01; 0.1; 1; 10; 100 and 1000 ppm of Ambon banana methanol extract and 0 ppm concentration as a control, each with three replications. The variables observed were individual deaths at every stage of development and morphological damage. The data obtained were analyzed using the analysis of variety and Duncan test with 95% confidence level. In addition, probit analysis was used to determine the value of LC50. The results showed that the study of toxicity indicates that in the further stage of development, the toxicity of methanol extract from Ambon banana hump was less toxic. Toxicity of Ambon banana hump methanol extract was highest in egg with LC50 value of 314,852 ppm. The methanol extract of banana Ambon has the morphological destructive activity in all development stages of Ae. aegypti.Abstrak. Nyamuk Ae. aegypti merupakan vektor utama virus dengue penyebab demam berdarah dengue (DBD) di Indonesia. Upaya pencegahan utama adalah dengan mengendalikan keberadaan nyamuk Ae. aegypti. Tanaman pisang diketahui memiliki kandungan senyawa metabolit sekunder yang memiliki aktivitas sebagai insektisida nabati, termasuk bagian bonggol. Penelitian ini dilakukan untuk mengevaluasi daya toksisitas ekstrak metanol bonggol pisang ambon (Musa acuminata L. cv. Gros Michel) terhadap nyamuk vektor dengue Ae. aegypti. Metode penelitian yang digunakan adalah metode eksperimental dengan desain penelitian rancangan acak lengkap (RAL), perlakuan yang dicoba berupa konsentrasi ekstrak metanol bonggol pisang ambon 0,001; 0,01; 0,1; 1; 10; 100, 1000 ppm dan 0 ppm sebagai kontrol, masing-masing dengan tiga kali ulangan. Variabel yang diamati adalah kematian individu pada setiap tahap perkembangan dan kerusakan morfologi. Data yang diperoleh dianalisis menggunakan analisis ragam dan uji duncan dengan taraf kepercayaan 95%, dan untuk menentukan nilai LC50 menggunakan analisis probit dengan aplikasi SPSS 16. Hasil penelitian menunjukkan bahwa semakin lanjut tahap perkembangan nyamuk Ae. aegypti, ekstrak metanol bonggol pisang ambon semakin tidak toksik. Daya toksik ekstrak metanol bonggol pisang ambon paling tinggi pada telur dengan nilai LC50 314,852 ppm, dengan aktivitas berupa penghambatan penetasan telur, sedangkan paling rendah pada imago dengan nilai LC50 1755,077 ppm. Ekstrak metanol bonggol pisang ambon memiliki aktivitas merusak morfologi semua tahap perkembangan Ae. aegypti.

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Anti-inflammatory activity of Sabicea brevipes Wernharm (Rubiaceae)

Attah¹,

Ugwuagbo²,

Chinnam³

et al. 2022

PHAR

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Over the years, medicinal plants have been employed in the treatment of inflammation and related ailments. This study evaluated the anti-inflammatory potential of the aerial parts of S. brevipes. The extracts and fractions were further evaluated for anti-inflammatory activity in carrageenan-induced rat model at varying doses (200 and 400 mg/kg doses, orally) for 5 h of treatment. The result of the phytochemical screening showed the presence of alkaloids, terpenoids, glycosides, flavonoids and tannins in the aerial parts of the plant. The in vivo anti-inflammatory study exhibited inhibition of 42% and 44%, 47% and 36%, 33% and 31%, and 43% and 42% for methanol extract n-hexane fraction, ethyl acetate fraction, and methanol fraction, at 200 and 400 mg/kg doses, respectively. The positive control (diclofenac sodium) showed an inhibition value of 51% at 5 mg/kg dose. Finally, it is concluded that S. brevipes possesses anti-inflammatory potential which validates the enthnomedicinal claim of the plant.

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Phytochemical Screening and Preliminary TLC Characterization of Alkaloids in Sabicea brevies Root

Cited by 3 publications

References 8 publications

Rubia cordifolia L. Crude Aerial Part Extracts Show a High Potential as Antimalarials against Chloroquine Resistant Plasmodium falciparum Strains in vitro

Rubia cordifolia L. Crude Aerial Part Extracts Show a High Potential as Antimalarials against Chloroquine Resistant Plasmodium falciparum Strains in vitro

Studi Toksisitas: Ekstrak Metanol Bonggol Pisang Ambon (Musa acuminata L. cv. Gros Michel) terhadap Aedes aegypti (Diptera: Culcidae)

Anti-inflammatory activity of Sabicea brevipes Wernharm (Rubiaceae)

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Phytochemical Screening and Preliminary TLC Characterization of Alkaloids in Sabicea brevies Root

Cited by 3 publications

References 8 publications

Rubia cordifolia L. Crude Aerial Part Extracts Show a High Potential as Antimalarials against Chloroquine Resistant Plasmodium falciparum Strains in vitro

Rubia cordifolia L. Crude Aerial Part Extracts Show a High Potential as Antimalarials against Chloroquine Resistant Plasmodium falciparum Strains in vitro

Studi Toksisitas: Ekstrak Metanol Bonggol Pisang Ambon (Musa acuminata L. cv. Gros Michel) terhadap Aedes aegypti (Diptera: Culcidae)

﻿Anti-inflammatory activity of Sabicea brevipes Wernharm (Rubiaceae)

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Anti-inflammatory activity of Sabicea brevipes Wernharm (Rubiaceae)