Phytochemical profiling of hot and cold alcoholic extract from Spirulina platensis alga and Comparison between two extracts against multidrug -resistant bacteria

Abstract

Background: Bacterial infections are one of the prominent problems causing death, health troubles and physical disabilities all over the world. Objective: This study was aimed to compare between hot and cold alcoholic extract of Spirulina platensis. Materials and Methods: in regards to antibacterial efficacy against several multidrug-resistant Gram-positive and Gram-negative bacteria, Spirulina was isolated from a freshwater station located in Baghdad, then identified in consideration to molecular analysis and morphologically. algal extracts were prepared using 70% methanol through Soxhlet and maceration extraction methods, antibacterial activity for both algal extracts was carried out by using agar well diffusion assay against several bacteria (Staphylococcus aureus, Streptococcussp., Pseudomonas aeruginosa, Escherichia coli, Klebsiella sp. and Serratia marscesence), also antibiotic sensitivity was determined for five different antibiotics (Gentamycin, levofloxacin, Netilimicin, Meropeneme, Cefixime) against tested bacteria. Results: The results showed that hot methanolic extract gives higher inhibition zones than cold extract. Besides, GC-Mass assessments resulted to identify biologically active chemicals (36 in hot and 6 in cold) as well as many Phyto-compounds within algal extract respectively. Conclusions: hot alcoholic extract of Spirulina platensis a good and safe choice to treat diseases caused by multi drug-resistant human pathogenic bacteria.

Fatty Acids, Minerals Composition, and Antimicrobial Activity of the Blue-Green Microalga (Spirulina platensis ) Isolated from Qilyasan Stream, Sulaimani, Kurdistan, Iraq

“…This suggests that other organic solvents extracted from S. platensis have the potential to be studied and further used as antimicrobial agents. The results of a study concluded that the ethyl-acetate crude extract of S.platensis is effective against S.aureus but has no effect on E.coli, this disagrees with our study due to the fact that ethyl-acetate extract had no significant effect on either S.aureus or E.coli (Fayyad et al, 2022). Moreover, the inhibitory activity is influenced by the type of algae the effectiveness of the extraction technique, and the active intergradient (Lechuga Morente, 2021).…”

Phytochemical Characterization and Anticancer Screening of Cladophora goensis and Cladophora glomerata Extracts

“…After being finished, the extract was filtered, condensed with a rotary evaporator at a regulated temperature and lowered pressure of 50°C-60°C (100 mg/mL of concentration), and then stored at room temperature for later use. 13 Methanolic extracts of C. goensis and C. glomerata were subjected to GC-MS analysis.…”

Antifugall Activity and Qualitative Phytochemical Investigation of Spirogyra Aequinoctialis Hexanic Extract in Iraq

“…Alcoholic extract was made using soxhelet method of extraction [10]. The hexane used in this method dry the algal material into a powder.…”