Phytochemical Profile and Biological Activity of Endemic Sideritis sipylea Boiss. in North Aegean Greek Islands

Axiotis, Evangelos; Petrakis, Eleftherios A.; Halabalaki, Maria; Mitakou, Sofia

doi:10.3390/molecules25092022

Cited by 29 publications

(38 citation statements)

References 47 publications

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“…In vitro antityrosinase assay was a spectrophotometric analysis based on the methods previously described [ 33 , 34 , 35 ]. l -DOPA (3,4-dihydroxyphenylalanine) and L-tyrosine were using as the substrate, respectively.…”

Section: Methodsmentioning

confidence: 99%

Antimelanogenesis Effects of Leaf Extract and Phytochemicals from Ceylon Olive (Elaeocarpus serratus) in Zebrafish Model

et al. 2021

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The melanogenesis inhibition effect in zebrafish (Danio rerio) and antityrosinase activity of the ethanolic extract and its phytochemicals from Ceylon olive (Elaeocarpus serratus Linn.) leaves were investigated in this study. Among the leaf extract and four soluble fractions, the ethyl acetate soluble fraction exhibits the best antityrosinase and antimelanogenesis activities. One phenolic acid, gallic acid, and two flavonoids, myricetin and mearnsetin, are isolated from the active subfractions through the bioassay-guided isolation; their structures are elucidated based on the 1D and 2D NMR, FTIR, UV, and MS spectroscopic analyses. These compounds have significant antityrosinase activity whether using l-tyrosine or l-DOPA as the substrate; mearnsetin shows the optimal activity. In the enzyme kinetic investigation, both gallic acid and mearnsetin are the competitive-type inhibitors against mushroom tyrosinase, and myricetin acts as a mixed-type tyrosinase inhibitor. Leaf extract and an ethyl acetate soluble fraction show effective performance in the inhibition of melanin formation in zebrafish embryos. Mearnsetin also possesses a promising antimelanogenesis effect, which is superior to the positive control, arbutin. Results reveal that the Ceylon olive leaf extract and its phytochemicals, especially mearnsetin, have the potential to be used as antimelanogenesis and skin-whitening ingredients.

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Section: Methodsmentioning

confidence: 99%

Antimelanogenesis Effects of Leaf Extract and Phytochemicals from Ceylon Olive (Elaeocarpus serratus) in Zebrafish Model

et al. 2021

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“…The DPPH radical scavenging assay determines antioxidant activity using the stable free radical 2,2-diphenyl-1-picrylhydrazyl [ 24 ]. Initially, 190 μL of DPPH solution (314 μM, 12.4 mg dissolved in 100 mL of absolute ethanol) and 10 μL of the tested sample were dispensed into each well of 96-well microplate and were incubated for 30 min at room temperature, avoiding light exposure.…”

Section: Methodsmentioning

confidence: 99%

Phytochemical Analysis and Dermo-Cosmetic Evaluation of Cymbidium sp. (Orchidaceae) Cultivation By-Products

et al. 2021

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Cymbidium is one of the most popular genera in Orchidaceae family, commercialized either as loose flowers or as potted plants in floriculture worldwide. The non-marketable parts are typically discarded (e.g., unsuitable flowers, leaves, pseudobulbs, roots), generating an enormous quantity of unutilized biomass. The above by-products were studied through phytochemical analysis and investigated for their dermo-cosmetic potential. The initial antioxidant, anti-tyrosinase, anti-elastase, and anti-collagenase assays of the total extracts indicated that the pseudobulb and root ethyl acetate extracts were the most potent. Those extracts were then submitted to chromatographic separation leading to the isolation of 16 secondary metabolites (four phenanthrenes, three 1,4-phenanthrenquinones, three dibenzyls, two phenolic acid derivatives, two sterols, one dehydrodiconiferyl alcohol derivative, and one simple phenolic compound), including 6-hydroxy-5,7-dimethoxy-1,4-phenanthrenequinone (cymbisamoquinone), which was identified as a new natural product. In parallel, 48 metabolites were identified by UPLC-HRMS analysis of the extracts. The biological evaluation of the isolated compounds revealed that gigantol and tristin present important anti-tyrosinase activity, while bulbophyllanthrin, 3-hydroxy-2,4,7-trimethoxy-phenanthrene, marylaurencinol A, 5-hydroxy-2-methoxy-1,4-phenanthrenequinone, and ephemeranthroquinone B show dose-dependent anti-collagenase activity. In contrast to isolated metabolites, which may act selectively on specific enzymes, the initial total extracts exhibited inhibitory activity against tyrosinase, elastase, and collagenase enzymes, thus showing better prospects for use in dermo-cosmetic formulations.

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“…The characterisation of foods’ constituents, quality or chemical markers and dereplication of known compounds are amongst our research axis and applications. So, we have also elaborated on the identification of key metabolites in different types of chocolate, mountain tea decoctions, saffron, Chios mastic gum, as well as on the very characteristic in Mediteranean diet edible greens (chorta) such as “stamnagathi” [ 66 , 69 , 99 , 100 , 101 , 102 , 103 ].…”

Section: Foodomicsgr_ri Plan and Strategymentioning

confidence: 99%

“…A total of 489 proteins of diverse functions were detected and were linked to the identity of the product [98] 3.5.2. NMR Technology Consortium members have developed several analytical protocols based on 1 H and 13 C NMR spectroscopy for metabolite profiling of a series of different food products [63,[99][100][101][102][103][104]. A special protocol based on 31 P NMR spectroscopy after chemical derivatization was developed and was used to profile minor glyceride components in olive oil [58,59], and was subsequently applied successfully for the analysis of a variety of foods [105].…”

Section: Lc-ms and Gc-ms Technologymentioning

confidence: 99%

FoodOmicsGR_RI: A Consortium for Comprehensive Molecular Characterisation of Food Products

et al. 2021

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The national infrastructure FoodOmicsGR_RI coordinates research efforts from eight Greek Universities and Research Centers in a network aiming to support research and development (R&D) in the agri-food sector. The goals of FoodOmicsGR_RI are the comprehensive in-depth characterization of foods using cutting-edge omics technologies and the support of dietary/nutrition studies. The network combines strong omics expertise with expert field/application scientists (food/nutrition sciences, plant protection/plant growth, animal husbandry, apiculture and 10 other fields). Human resources involve more than 60 staff scientists and more than 30 recruits. State-of-the-art technologies and instrumentation is available for the comprehensive mapping of the food composition and available genetic resources, the assessment of the distinct value of foods, and the effect of nutritional intervention on the metabolic profile of biological samples of consumers and animal models. The consortium has the know-how and expertise that covers the breadth of the Greek agri-food sector. Metabolomics teams have developed and implemented a variety of methods for profiling and quantitative analysis. The implementation plan includes the following research axes: development of a detailed database of Greek food constituents; exploitation of “omics” technologies to assess domestic agricultural biodiversity aiding authenticity-traceability control/certification of geographical/genetic origin; highlighting unique characteristics of Greek products with an emphasis on quality, sustainability and food safety; assessment of diet’s effect on health and well-being; creating added value from agri-food waste. FoodOmicsGR_RI develops new tools to evaluate the nutritional value of Greek foods, study the role of traditional foods and Greek functional foods in the prevention of chronic diseases and support health claims of Greek traditional products. FoodOmicsGR_RI provides access to state-of-the-art facilities, unique, well-characterised sample sets, obtained from precision/experimental farming/breeding (milk, honey, meat, olive oil and so forth) along with more than 20 complementary scientific disciplines. FoodOmicsGR_RI is open for collaboration with national and international stakeholders.

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Phytochemical Profile and Biological Activity of Endemic Sideritis sipylea Boiss. in North Aegean Greek Islands

Cited by 29 publications

References 47 publications

Antimelanogenesis Effects of Leaf Extract and Phytochemicals from Ceylon Olive (Elaeocarpus serratus) in Zebrafish Model

Antimelanogenesis Effects of Leaf Extract and Phytochemicals from Ceylon Olive (Elaeocarpus serratus) in Zebrafish Model

Phytochemical Analysis and Dermo-Cosmetic Evaluation of Cymbidium sp. (Orchidaceae) Cultivation By-Products

FoodOmicsGR_RI: A Consortium for Comprehensive Molecular Characterisation of Food Products

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