Phytochemical and in vitro antioxidant evaluation of different fractions of Amaranthus graecizans subsp. silvestris (Vill.) Brenan.

Ishtiaq, Saiqa; Ahmad, Mansoor; Hanif, Uzma; Akbar, Shehla; Mehjabeen,; Kamran, Sairah Hafeez

doi:10.1016/s1995-7645(14)60256-x

Cited by 29 publications

(20 citation statements)

References 17 publications

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“…In addition, the fractions with a neutral polarity contained more total flavonoid content than the other fractions. The result of the phytochemical analysis indicated that considerable quantities of common bioactive components such as terpenoids, alkaloids, phenolics, flavonoids, and saponins were concentrated in the medium polar and polar fractions such as the chloroform, ethyl acetate, and butanol fractions ( 15 ). Other study showed that the total polyphenol content was higher in the ethyl acetate fraction than that in the water fractions of Flammulina velutipes (3.17~3.50 mg GAE/g) and Lyophyllum decastes (1.52~2.92 mg GAE/g) ( 22 ).…”

Section: Resultsmentioning

confidence: 99%

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Analysis of Antioxidant and Anti-Inflammatory Activities of Solvent Fractions from Rhynchosia nulubilis Cultivated with Ganoderma lucidum Mycelium

Park¹,

Kim²

2017

pnf

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In this study, the crude ethanol Rhynchosia nulubilis cultivated with Ganoderma lucidum mycelium (RNGM) extract was solvent fractionated with organic solvents such as n-hexane, chloroform, ethyl acetate, and water. The anticancer activities, anti-inflammatory activity total polyphenols, total flavonoids, isoflavones, and β-glucan of the solvent fractions of RNGM were studied. The ethyl acetate fraction showed the highest 2,2-diphenyl-1-picrylhydrazyl scavenging activity of 76.60% at 800 μg/mL. The ethyl acetate fraction also showed higher antioxidant activity in 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) and ferric reducing ability of plasma assays compared to the other fractions. In addition, this study confirmed that the ethyl acetate fraction strongly inhibited nitric oxide production. The ethyl acetate fraction had the highest amount of total polyphenol and total flavonoid (65.33 mg gallic acid equivalent/g and 18.50 mg quercetin equivalent/g, respectively). The ethyl acetate fraction (13.02%) showed the highest amount of total β-glucan, followed by the water (6.32%), chloroform (1.43%), and n-hexane fraction (0.85%). Therefore, it is suggested that the ethyl acetate fraction of Rhynchosia nulubilis cultivated with Ganoderma lucidum mycelium may be potential natural sources for nutritional and pharmaceutical applications.

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Section: Resultsmentioning

confidence: 99%

“…The total phenolic content was determined using the Folin-Denis method ( 14 ) with slight modifications. The total flavonoid content of the fractions was determined using the aluminum chloride test according to the method of Saiqa et al ( 15 ).…”

Section: Methodsmentioning

confidence: 99%

Analysis of Antioxidant and Anti-Inflammatory Activities of Solvent Fractions from Rhynchosia nulubilis Cultivated with Ganoderma lucidum Mycelium

Park¹,

Kim²

2017

pnf

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“…The concentration of total phenolic content in plant extracts was calculated from the calibration curve of Gallic acid and it was expressed as Gallic acid equivalents/gram fresh weight (Ishtiaq et al, 2014). Each experiment had three replicates.…”

Section: Phytochemical Evaluation (Quantitative Methods) Analysis Of mentioning

confidence: 99%

Phytochemical and HPTLC Studies on Fruit Extracts of Momordica cymbalaria Fenzl, a Medicinally Important Plant

Shantakani

Devi

et al. 2017

Not Sci Biol

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Preliminary screening of phytochemicals is a valuable step in the detection of bioactive principles present in medicinal plants, which subsequently may lead to drug discovery and development. In the present study, chief phyto-constituents of the Momordica cymbalaria were identified. Preliminary screening of phytochemicals was done by using three extraction methods: maceration, soxhlation and reflux with hexane, ethyl acetate and methanol. All the nine extracts showed the presence of flavonoids, phenols, steroids and terpenoids that were obtained by the three extraction methods. The present work also attempted to optimize the HPTLC fingerprint profiles of secondary metabolites in hexane, ethyl acetate and methanolic fruit extracts of M. cymabalaria. Soxhlated methanolic fruit extract was rich in secondary compounds and exhibited more biological activity than the other extracts. Hence future study is needed to isolate potential bioactive principle from fruit extract of M. cymbalaria.

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“…The ferric reducing antioxidant power (FRAP) of CO was determined using Benzie and Strain's (1996) method as described by Ishtiaq et al (2014). The FRAP reagent was prepared fresh using a 300 mM acetate buffer at pH 3.6 (3.1 g sodium acetatetrihydrate, 16 ml glacial acid made up to 1 : 1 with distilled water), 10 mM (2,4,6-tris (2-pyridyl)-s-triazine) in 40 mM HCl, and 20 mM FeCl 3 @ 6H 2 O in the ratio of 10 : 1 : 1 to afford the working reagent.…”

Section: Ferric Reducing Antioxidant Power Of Comentioning

confidence: 99%

“…The FRAP assay measures the total antioxidant capacity in a sample by considering the oxidation-reduction potential (Ishtiaq et al, 2014). The interaction of antioxidants with the ferric tripyridyltriazine complex favors the reduction of the complex to produce ferrous tripyridyltriazine with an intense blue color (Gülçin, 2012).…”

Section: Antioxidant and Metal Ion Chelating Activity Of Comentioning

confidence: 99%

Antioxidant property and GCMS profile of oil extracted from Cocos nucifera using a fermentation method

Adenike¹,

Adegbola²,

Fadahunsi³

2019

bta

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Cocos nucifera L. is known as a tree of life because of its economic, domestic, and nutritional usefulness. Coconut oil (CO), which is derived from Cocos nucifera L., has received considerable attention because of its reported folkloric, nutritional, biological, and pharmacological properties. We previously reported the outstanding physicochemical properties of CO; therefore, we analyzed its antioxidant activity and chemical composition in this study. CO was extracted using a fermentation method with and without applying heat. Its antioxidant activity was investigated using the DPPH free radical scavenging method, the metal chelation capacity, the reduction of antioxidant power, and the nitric oxide scavenging capacity index. The compounds were identified using GC-MS. The data were expressed as a mean ± SEM and analyzed by one-way analysis of variance (ANOVA) using SPSS 21.0 with data being considered significant at P < 0.05. The results showed that CO demonstrated a concentration-dependent DPPH radical scavenging activity and nitric oxide scavenging capacity index, with the highest activity in the heat-extracted virgin CO (HEVCO). The ferric-reducing antioxidant power and metal chelation capacity significantly varied (P < 0.05) between the HEVCO and the cold extracted virgin CO (CEVCO). The GCMS analysis of virgin CO identified important active compounds. The results revealed a higher content of phenolic compounds in HEVCO compared to CEVCO. In conclusion, applying heat favored incorporating phenolic compounds into CO and consequently improved the antioxidant potential of HEVCO compared to CEVCO.

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Phytochemical and in vitro antioxidant evaluation of different fractions of Amaranthus graecizans subsp. silvestris (Vill.) Brenan.

Abstract: The results indicate that A. graecizans subsp. silvestris has antioxidant potential and can be utilized as a natural source of antioxidant.

Cited by 29 publications

References 17 publications

Analysis of Antioxidant and Anti-Inflammatory Activities of Solvent Fractions from Rhynchosia nulubilis Cultivated with Ganoderma lucidum Mycelium

Analysis of Antioxidant and Anti-Inflammatory Activities of Solvent Fractions from Rhynchosia nulubilis Cultivated with Ganoderma lucidum Mycelium

Phytochemical and HPTLC Studies on Fruit Extracts of Momordica cymbalaria Fenzl, a Medicinally Important Plant

Antioxidant property and GCMS profile of oil extracted from Cocos nucifera using a fermentation method

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