Analysis of Antioxidant and Anti-Inflammatory Activities of Solvent Fractions from Rhynchosia nulubilis Cultivated with Ganoderma lucidum Mycelium

Park, MiHye; Kim, Meera

doi:10.3746/pnf.2017.22.4.365

Cited by 9 publications

(4 citation statements)

References 26 publications

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“…us, the highest free radical scavenging activity of the ethyl acetate fraction could be due to the presence of an increased amount of those antioxidant metabolites in higher proportions compared with the crude extract and aqueous fractions. is is similar to another finding where the ethyl acetate fraction of a different plant showed the highest radical scavenging activity [44].…”

Section: Discussionsupporting

confidence: 91%

Cardioprotective Effect of Croton macrostachyus Stem Bark Extract and Solvent Fractions on Cyclophosphamide-Induced Cardiotoxicity in Rats

Ayza

Balasubramanian²,

Berhe

2020

Evidence-Based Complementary and Alternative Medicine

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Context. Croton macrostachyus Hochst. ex Delile (Euphorbiaceae) has been used in traditional medicine to manage heart failure and other heart diseases in Ethiopia. Objective. To evaluate the antioxidant and cardioprotective activities of stem bark extract and solvent fractions of Croton macrostachyus on cyclophosphamide-induced cardiotoxicity in rats. Materials and Methods. DPPH free radical scavenging assay method was used to determine antioxidant activity whereas Sprague-Dawley rats were used to evaluate the cardioprotective activity. Except for the normal control, all groups were subjected to cyclophosphamide (200 mg/kg, i.p.) toxicity on the first day. Enalapril at 10 mg/kg was used as a reference. The hydromethanolic crude extract (100, 200, and 400 mg/kg) and aqueous and ethyl acetate fractions (100 and 200 mg/kg, each) were administered for 10 days. The cardioprotective activities were evaluated using cardiac biomarkers such as Troponin I, aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), total cholesterol (TC), triglyceride (TG), and histopathological studies of heart tissue. Results. Crude extract and ethyl acetate and aqueous fractions exhibited free radical scavenging activities at IC50 of 594 μg/mL, 419 μg/mL, and 716 μg/mL, respectively. Crude extract at 400 mg/kg decreased the levels of troponin, AST, ALT, and ALP to 0.29 ± 0.06 ng/mL, 103.00 ± 7.63 U/L, 99.80 ± 6.18 U/L, and 108.80 ± 8.81 U/L, respectively. In addition, ethyl acetate fraction at 200 mg/kg decreased the levels of troponin, AST, ALT, and ALP to 0.22 ± 0.02 ng/mL, 137.00 ± 14.30 U/L, 90.33 ± 6.13 U/L, and 166.67 ± 13.50 U/L, respectively, compared with the cyclophosphamide control group. Conclusions. Croton macrostachyus possesses cardioprotective activities and it could be a possible source of treatment for cardiotoxicity induced by cyclophosphamide.

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Section: Discussionsupporting

confidence: 91%

Cardioprotective Effect of Croton macrostachyus Stem Bark Extract and Solvent Fractions on Cyclophosphamide-Induced Cardiotoxicity in Rats

Ayza

Balasubramanian²,

Berhe

2020

Evidence-Based Complementary and Alternative Medicine

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“…The TE/L. In contrast, betel and kaduk extracted by EA (1384.40 mg TE/L) and HE (1191.48 g TE/L) solvents exhibited higher FRAP activity than did those extracted by W. Similar results were also reported in the studies of Guleria et al50 on the fractions of Terminalia chebula fruit and Park et al51 on the fractions of Rhynchosia nulubilis cultivated with Ganoderma lucidum.…”

supporting

confidence: 83%

Comparison of the total phenolic content and antioxidant and antibacterial activities of different fractions obtained from selected plant leaves native to Viet Nam

Nguyen,

Nguyen

2023

Sci. Tech. Dev. J.

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In this study, five types of plant extracts in Vietnam were selected to compare phenolic content, antioxidant and antibacterial activities, respectively Gymnanthemum amygdalinum (bitter leaf), Piper betle (betel), Pseuderanthemum bracteatum (Imlay), Piper sarmentosum (kaduk), and Paederia tomentosa (stinkvine). Five types of leaves were fractionally extracted with n-hexane (HE), ethyl acetate (EA) and water (W) solvents. The antioxidant activity was compared based on free radical scavenging capacity (DPPH, ABTS), iron reducing capacity (FRAP) and iron chelation capacity (FIC). At the same time, the total phenolic content (TPC) was also compared by the Folin-Ciocalteau method. The results demonstrate antioxidant activity (DPPH), the EA fraction of betel leaf was the best, followed by four extracts of bitter leaf > kaduk > Imlay > stinkvine. Similarly, the EA fraction of betel leaf also showed the highest FRAP and ABTS iron-reducing activity. A correlation between phenolic content and antioxidant activities of leaf extracts was also investigated. Regarding antibacterial activity, betel leaf in all fractions showed the highest antibacterial activity against most gram (+) and gram (-) organisms through diffuse on agar plate test. Meanwhile, bitter leaf showed the lowest antibacterial activity in both EA and W fractions.

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“…The ethanol extract was then subjected to a liquid–liquid fractionation, as described by Lestari et al (2019) and Park and Kim (2017), with minor changes [ 47 , 75 ]. Dried ethanol extract (5 g) was dissolved in a mixture of ethanol (45 mL) and distilled water (5 mL) to produce the crude ethanol extract.…”

Section: Methodsmentioning

confidence: 99%

The Antiproliferative Effect of Chloroform Fraction of Eleutherine bulbosa (Mill.) Urb. on 2D- and 3D-Human Lung Cancer Cells (A549) Model

2023

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Since lung cancer is the leading cause of cancer-related death worldwide, research is being conducted to discover anticancer agents as its treatment. Eleutherine bulbosa, a Dayak folklore medicine, exhibited anticancer effects against several cancer cells; however, its anticancer potency against lung cancer cells has not been explored yet. This study aims to determine the anticancer potency of E. bulbosa bulbs against lung cancer cells (A549) using 2D and 3D culture models, as well as determine its active compounds using gas chromatography-mass spectrometry (GC-MS) analysis. Three fractions of E. bulbosa bulbs, namely chloroform, n-hexane, and ethyl acetate, were tested for cytotoxicity using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium-bromide (MTT) and CellTiter-Glo. The antiproliferative effects of the most cytotoxic fraction against the 2D culture model were determined by a clonogenic survival assay and propidium iodide/Hoechst 33342 double staining, whereas the effects against the 3D culture model were determined by microscopy, flow cytometry, and gene expression analysis. The chloroform fraction is the most cytotoxic against A549 cells than other fractions, and it inhibited colony formation and induced apoptosis of A549 cells. The chloroform fraction also inhibited the growth of the A549 spheroid by suppressing the spheroid size, inducing apoptosis, reducing the proportion of CD44 lung cancer stem cells, causing arrest at the S phase of the cell cycle, and suppressing the expression of the SOX2 and MYC genes. Furthermore, the GC-MS analysis detected 20 active compounds in the chloroform fraction, including the major compounds of eleutherine and isoeleutherine. In conclusion, the chloroform fraction of E. bulbosa bulbs exhibit its antiproliferative effect on 2D and 3D culture models of A549 cells, suggesting it could be a lung cancer chemopreventive agent.

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Analysis of Antioxidant and Anti-Inflammatory Activities of Solvent Fractions from Rhynchosia nulubilis Cultivated with Ganoderma lucidum Mycelium

Cited by 9 publications

References 26 publications

Cardioprotective Effect of Croton macrostachyus Stem Bark Extract and Solvent Fractions on Cyclophosphamide-Induced Cardiotoxicity in Rats

Cardioprotective Effect of Croton macrostachyus Stem Bark Extract and Solvent Fractions on Cyclophosphamide-Induced Cardiotoxicity in Rats

Comparison of the total phenolic content and antioxidant and antibacterial activities of different fractions obtained from selected plant leaves native to Viet Nam

The Antiproliferative Effect of Chloroform Fraction of Eleutherine bulbosa (Mill.) Urb. on 2D- and 3D-Human Lung Cancer Cells (A549) Model

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