Phytochemical and biological activities of ethanolic extract of C. hirsuta leaves

Abstract

This study investigated the phytochemical and biological activities like antioxidant, antibacterial and cytotoxitc effects of C. hirsuta (Synonym- Senna hirsuta) leaf extracted with exhanol. Qualitative phytochemical analyses were accomplished by established methods. Antioxidant, antibacterial and cytotoxic effects were, respectively, measured by DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging method, disc diffusion method and brine shrimp lethality bioassay. The presence of alkaloids, glycosides, anthraquinone glycosides and flavonoids were detected in the leaf extract. The extract showed significant (p < 0.01) radical scavenging effect (IC50, 200.96 ± 0.85 ?g/ml) in comparison to ascorbic acid (IC50, 1.24 ± 0.08 ?g/ml). The extract also showed significant (p < 0.05) zone of inhibitions against Gram-positive Bacillus cereus (13 ± 1.05 mm) and Bacillus megaterium (9 ± 0.85 mm), and Gram-negative Vibrio cholera (20 ± 0.68 mm), Escherichia coli (8 ± 0.92 mm), Pseudomonas aeroginosa (15 ± 1.03 mm), Salmonella paratyphi (9 ± 0.79 mm) and Shigella dysenteriae (8 ± 0.88 mm). In cytotoxic assay, the extract showed the LC50 value 315.5 ?g/ml which was statistically significant (p < 0.01) compared to reference cytotoxic agent vincristine sulfate (LC50, 38.99 ?g/ml). DOI: http://dx.doi.org/10.3329/bjsir.v48i1.15413 Bangladesh J. Sci. Ind. Res. 48(1), 43-50, 2013

Evaluation of cytotoxic and antioxidant activity of different fractions of methanolic extract of Baccaurea ramiflora (Lour.) fruits

“…They might have antitumor activity as the cytotoxicity of plant material is considered to be caused by the presence of antitumor compounds (Rahman and Ahmed, 2013). The brine shrimp lethality bioassay also indicates antifungal effects, pesticidal effects, teratogenic effects, toxicity to environment and many more (Rahman et al, 2014).…”

Investigation of the Larvicidal Potential of Silver Nanoparticles againstCulex quinquefasciatus: A Case of a Ubiquitous Weed as a Useful Bioresource

“…This was confirmed by the gradual color change of the final mixture from light yellow to yellowish brown (Figure 2(a) inset) indicating the formation of AgNPs [15]. Phytochemicals like tannins, alkaloids, glycosides, and flavonoids in the leaf extract [43,45] are responsible for the reduction and stabilization of Ag + to Ag 0 nanoparticles. The surface plasmon resonance (SPR) band was observed at 440 nm for 30 ∘ C and 420 nm for both 50 ∘ C and 80 ∘ C, respectively.…”

“…The petals of C. hirsuta were reported to contain cardiac glycosides [41] while the presence of tannins [42], phlobatannins [43], alkaloids, glycosides, anthraquinone glycosides, steroids, and flavonoids has been reported in the leaf extract [44]. The antioxidant, antibacterial, and cytotoxic activities of C. hirsuta leaf extract have been investigated [45] and high quantities of carbohydrate and proteins have been reported [43]. However, there have been no reports on the biosynthesis of AgNPs using C. hirsuta.…”

In Vitro Antimicrobial Activity and GC-MS Analysis of Crude Aqueous Methanolic Extract Produced from Leaves of Eucalyptus species

“…The phytochemical chemical tests were carried out according to (18)(19)(20)(21)(22) . The solution of aqueous methanolic extract was performed by re-dissolving the solid extract in aqueous methanol (80%).…”