This study investigated the phytochemical and biological activities like antioxidant, antibacterial and cytotoxitc effects of C. hirsuta (Synonym- Senna hirsuta) leaf extracted with exhanol. Qualitative phytochemical analyses were accomplished by established methods. Antioxidant, antibacterial and cytotoxic effects were, respectively, measured by DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging method, disc diffusion method and brine shrimp lethality bioassay. The presence of alkaloids, glycosides, anthraquinone glycosides and flavonoids were detected in the leaf extract. The extract showed significant (p < 0.01) radical scavenging effect (IC50, 200.96 ± 0.85 ?g/ml) in comparison to ascorbic acid (IC50, 1.24 ± 0.08 ?g/ml). The extract also showed significant (p < 0.05) zone of inhibitions against Gram-positive Bacillus cereus (13 ± 1.05 mm) and Bacillus megaterium (9 ± 0.85 mm), and Gram-negative Vibrio cholera (20 ± 0.68 mm), Escherichia coli (8 ± 0.92 mm), Pseudomonas aeroginosa (15 ± 1.03 mm), Salmonella paratyphi (9 ± 0.79 mm) and Shigella dysenteriae (8 ± 0.88 mm). In cytotoxic assay, the extract showed the LC50 value 315.5 ?g/ml which was statistically significant (p < 0.01) compared to reference cytotoxic agent vincristine sulfate (LC50, 38.99 ?g/ml). DOI: http://dx.doi.org/10.3329/bjsir.v48i1.15413 Bangladesh J. Sci. Ind. Res. 48(1), 43-50, 2013
Seaweeds are fresh sources of phytochemical compounds with immense medicinal potential, which have attracted the attention of agriculture, aquaculture and the pharmaceutical industry. The aim of this study was to test the antioxidant, antibacterial and cytotoxic activity and screening the phytochemical properties of methanol crude extract from two green seaweeds Caulerpa racemosa (Forsskål) J.Agardh and Ulva (Enteromorpha) intestinalis L. The antioxidant and cytotoxic activity of these seaweeds were assessed by 2,2diphenyl, 1-picryl hydrazyl (DPPH) free radical scavenging technique and brine shrimp lethality bioassay method, respectively. The antibacterial activities against Vibrio fluvialis was determined using the standard disc diffusion method. The U. intestinalis showed higher total phenolic content with the value of 149.87 ± 18.17 mg of GAE/g than 73.95 ± 16.09 mg of GAE/g of C. racemosa. However, the recorded inhibition concentration (IC50) to corresponding standards ascorbic acid of C. racemosa and U. intestinalis were 119.62 and 34.274 μg/mL, respectively. The values were statistically significant (p <0.05) compared to the reference antioxidative agent ascorbic acid. In antibacterial assay, C. racemosa extract showed higher inhibition zone (22.65 ± 0.58 mm) than U. intestinalis (17± 0.28 mm). Moreover, U. intestinalis exhibited a lower LC50 value than C. racemosa in cytotoxic activity. Phytochemical analysis revealed that these two seaweeds have alkaloids, flavonoids, glycosides, phenols, saponins, steroids and tannins.
Bangladesh J. Sci. Ind. Res.55(4), 237-244, 2020
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.