Evaluation of cytotoxic and antioxidant activity of different fractions of methanolic extract of Baccaurea ramiflora (Lour.) fruits

Abstract

Chloroform and petroleum ether soluble fractions of the crude methanolic extract of the fruits of Baccaurea ramiflora (Lour.) have been investigated for the evaluation of cytotoxic and antioxidant activity. Brine shrimp lethality bioassay was used to evaluate the cytotoxic activity and free radical scavenging activity method was used to evaluate antioxidant activity. In brine shrimp lethality bioassay, the chloroform soluble fraction showed greater cytotoxic activity (LC50 value 74.2 µg/ml) than petroleum ether soluble fraction (LC50 value 129 µg/ml) compared to the standard drug vincristine sulfate (LC50 value 0.54 µg/ml). In free radical scavenging activity method (DPPH assay method), the chloroform soluble fraction showed greater antioxidant activity (IC50 value 49.78 µg/ml) than petroleum ether soluble fraction (IC50 value 75.31 µg/ml) compared to the standard antioxidant butylated hydroxytoluene (IC50 value 24.51µg/ml). This study reveals that Baccaurea ramiflora fruit possesses cytotoxic and antioxidant activity hence can be a potential source for the isolation of active principle(s) for different therapies.International Current Pharmaceutical Journal, May 2015, 4(6): 386-389

<i>Baccaurea ramiflora</i>: Isolation of Aldehydes and in Vitro Biological Investigations

“…depicted mortality rate of different fractions of Baccaurea ramiflora while Table 5, Figure5showed different degree of lethality of plant extracts of Baccaurea ramiflora to Brine shrimp. Among the fractions, chloroform soluble fraction was found to be most toxic to brine shrimp and petroleum ether soluble fraction showed moderate toxicity compared to anticancer drug vincristine sulphate, which support the previous data about this[9] [10][20]. Further in vivo acute oral toxicity study can confirm whether this toxicity level is harmful for susceptible biological systems or not.…”

“…have been isolated from different parts of Baccaurea ramiflora. As well as analgesic activity from seeds [7], anthelmintic from the whole plant [8], antioxidant activity from fruits [9], cytotoxicity from fruits [10] and hypoglycemic and hypolipidemic activity from the leaves [11] of Baccaurea ramiflora were mentioned. Further research can identify whether there are any unidentified bioactive principles.…”

Estimation of Antioxidant and Cytotoxicity Activities of Extracts Obtained from the Leaves of Folk Medicinal Plant Benkara malabarica (Lam)

“…Highest extraction power and polarity of methanol can be inferred for the higher activities of methanol extracts. 47 The type of phytochemicals, present in the extracts varies with use of different solvents. Each solvent has specific influence on the extraction of phytochemicals from plant parts.…”

Dietary Fiber Profiling of Different Parts of Burmese Grape (Baccaurea ramiflora) and Their Application

“…Due to its high content of vitamin C, protein, and iron; B. ramiflora fruit is used as a novel food additive also [2] . Amin and Nabi also revealed that Baccaurea ramiflora fruit possesses cytotoxic properties and antioxidant activity so that, this fruit could prevent the diseases caused by oxidative stress and cancer [3] . The fruit is similar to berry and the edible portion is aril which is covered by the leathery rind.…”