Background and Aim:Nanoparticles can bypass conventional physiological ways of nutrient distribution and transport across tissue and cell membranes, as well as protect compounds against destruction prior to reaching their targets. In ovo administration of nanoparticles, may be seen as a new method of nano-nutrition, providing embryos with an additional quantity of nutrients. The aim of the study is to examine the effect of in ovo supplementation of nano forms of zinc, copper and selenium on the hatchability and post hatch performance of broiler chicken.Materials and Methods:Nano form of zinc at 20, 40, 60 and 80 µg/egg, nano form of copper at 4, 8, 12 and 16 µg/egg and nano form of selenium at 0.075, 0.15, 0.225 and 0.3 µg/egg were in ovo supplemented (18th day incubation, amniotic route) in fertile broiler eggs. Control group in ovo fed with normal saline alone was also maintained. Each treatment had thirty replicates. Parameters such as hatchability, hatch weight and post hatch performance were studied.Results:In ovo feeding of nano minerals were not harmful to the developing embryo and did not influence the hatchability. Significantly (p<0.05) best feed efficiency for nano forms of zinc (2.16), copper (2.46) and selenium (2.51) were observed, when 40, 4 and 0.225 µg/egg respectively were in ovo supplemented. Except in nano form of copper at 12 µg per egg which had significantly (p<0.05) highest breast muscle percentage there was no distinct trend to indicate that dressing percentage or breast muscle yield was influenced in other treatments.Conclusion:Nano forms of zinc, copper and selenium can be prepared at laboratory conditions. In ovo feeding of nano forms of zinc, copper and selenium at 18th day of incubation through amniotic route does not harm the developing embryo, does not affect hatchability.
The stratospheric ozone depletion and enhanced solar ultraviolet-B (UV-B) irradiance may have adverse impact on living organisms. The impact of UV-B radiation (UV-B, 280~320nm) on growth, biochemical and antioxidant enzyme activity was studied in Indigofera tinctoria (L.) seedling, commonly used as a green manure. The supplementary UV-B radiation significantly decreased the growth, development and changes in UV-B absorbing compounds such as anthocyanin and flavonoids. The antioxidant enzymes were unaffected and showed an enhanced activities of peroxidase, superoxide dismutase, polyphenoloxidase and phenylalanine ammonia-lyase except catalase in UV-B irradiated seedling. Indigofera tinctoria seedling tries to counteract high level of reactive oxygen species produced under UV-B stress through the increased activities of antioxidant enzyme. The results suggest that Indigofera tinctoria is resistant to UV-B radiation damage and the possible negative effect of additional UV-B radiation on the growth of seedling may have been effectively balanced by the UV-B radiation stress through increase in UV-absorbing compound and antioxidant enzymes.
Olfaction is the response to odors and is mediated by a class of membrane-bound proteins called olfactory receptors (ORs). An understanding of these receptors serves as a good model for basic signal transduction mechanisms and also provides important clues for the strategies adopted by organisms for their ultimate survival using chemosensory perception in search of food or defense against predators. Prior research on cross-genome phylogenetic analyses from our group motivated the addressal of conserved evolutionary trends, clustering, and ortholog prediction of ORs. The database of olfactory receptors (DOR) is a repository that provides sequence and structural information on ORs of selected organisms (such as Saccharomyces cerevisiae, Drosophila melanogaster, Caenorhabditis elegans, Mus musculus, and Homo sapiens). Users can download OR sequences, study predicted membrane topology, and obtain cross-genome sequence alignments and phylogeny, including three-dimensional (3D) structural models of 100 selected ORs and their predicted dimer interfaces. The database can be accessed from http://caps.ncbs.res.in/DOR. Such a database should be helpful in designing experiments on point mutations to probe into the possible dimerization modes of ORs and to even understand the evolutionary changes between different receptors.
G-protein coupled receptors (GPCRs) are one of the largest groups of membrane proteins and are popular drug targets. The work reported here attempts to perform cross-genome phylogeny on GPCRs from two widely different taxa, human versus C. elegans genomes and to address the issues on evolutionary plasticity, to identify functionally related genes, orthologous relationship, and ligand binding properties through effective bioinformatic approaches. Through RPS blast around 1106 nematode GPCRs were given chance to associate with previously established 8 types of human GPCR profiles at varying E-value thresholds and resulted 32 clusters were illustrating co-clustering and class-specific retainsionship. In the significant thresholds, 81% of the C. elegans GPCRs were associated with 32 clusters and 27 C. elegans GPCRs (2%) inferred for orthology. 177 hypothetical proteins were observed in cluster association and could be reliably associated with one of 32 clusters. Several nematode-specific GPCR clades were observed suggesting lineage-specific functional recruitment in response to environment.
Multiple sequence alignments become biologically meaningful only if conserved and functionally important residues and
secondary structural elements preserved can be identified at equivalent positions. This is particularly important for transmembrane
proteins like G-protein coupled receptors (GPCRs) with seven transmembrane helices. TM-MOTIF is a software package and an
effective alignment viewer to identify and display conserved motifs and amino acid substitutions (AAS) at each position of the
aligned set of homologous sequences of GPCRs. The key feature of the package is to display the predicted membrane topology for
seven transmembrane helices in seven colours (VIBGYOR colouring scheme) and to map the identified motifs on its respective
helices /loop regions. It is an interactive package which provides options to the user to submit query or pre-aligned set of GPCR
sequences to align with a reference sequence, like rhodopsin, whose structure has been solved experimentally. It also provides the
possibility to identify the nearest homologue from the available inbuilt GPCR or Olfactory Receptor cluster dataset whose
association is already known for its receptor type.AvailabilityThe database is available for free at mini@ncbs.res.in
G-protein coupled receptors (GPCRs) belong to biologically important and functionally diverse and largest super family of
membrane proteins. GPCRs retain a characteristic membrane topology of seven alpha helices with three intracellular, three
extracellular loops and flanking N' and C' terminal residues. Subtle differences do exist in the helix boundaries (TM-domain), loop
lengths, sequence features such as conserved motifs, and substituting amino acid patterns and their physiochemical properties
amongst these sequences (clusters) at intra-genomic and inter-genomic level (please re-phrase into 2 statements for clarity). In the
current study, we employ prediction of helix boundaries and scores derived from amino acid substitution exchange matrices to
identify the conserved amino acid residues (motifs) as consensus in aligned set of homologous GPCR sequences. Co-clustered
GPCRs from human and other genomes, organized as 32 clusters, were employed to study the amino acid conservation patterns
and species-specific or cluster-specific motifs. Critical analysis on sequence composition and properties provide clues to connect
functional relevance within and across genome for vast practical applications such as design of mutations and understanding of
disease-causing genetic abnormalities.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.