Background and Aim:Nanoparticles can bypass conventional physiological ways of nutrient distribution and transport across tissue and cell membranes, as well as protect compounds against destruction prior to reaching their targets. In ovo administration of nanoparticles, may be seen as a new method of nano-nutrition, providing embryos with an additional quantity of nutrients. The aim of the study is to examine the effect of in ovo supplementation of nano forms of zinc, copper and selenium on the hatchability and post hatch performance of broiler chicken.Materials and Methods:Nano form of zinc at 20, 40, 60 and 80 µg/egg, nano form of copper at 4, 8, 12 and 16 µg/egg and nano form of selenium at 0.075, 0.15, 0.225 and 0.3 µg/egg were in ovo supplemented (18th day incubation, amniotic route) in fertile broiler eggs. Control group in ovo fed with normal saline alone was also maintained. Each treatment had thirty replicates. Parameters such as hatchability, hatch weight and post hatch performance were studied.Results:In ovo feeding of nano minerals were not harmful to the developing embryo and did not influence the hatchability. Significantly (p<0.05) best feed efficiency for nano forms of zinc (2.16), copper (2.46) and selenium (2.51) were observed, when 40, 4 and 0.225 µg/egg respectively were in ovo supplemented. Except in nano form of copper at 12 µg per egg which had significantly (p<0.05) highest breast muscle percentage there was no distinct trend to indicate that dressing percentage or breast muscle yield was influenced in other treatments.Conclusion:Nano forms of zinc, copper and selenium can be prepared at laboratory conditions. In ovo feeding of nano forms of zinc, copper and selenium at 18th day of incubation through amniotic route does not harm the developing embryo, does not affect hatchability.

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A 60 day study was carried out to determine the effect of protein supplement in Kano brown bucks. Twenty four bucks with initial weight of 10.25-12.33 kg were grouped into three of eight bucks each and were randomly assigned to the three treatment diets in a completely randomized design. Prior to this, concentrate diets were formulated to contain 15%, 30% and 45% cottonseed cake (CSC) as the protein supplement in the dietary treatments. Crop residues formed the basal diet and were fed ad libitum. Alongside, fresh clean water was provided to goats ad libitum. Parameters determined during the experiment were feed intake, daily weight changes, Feed cost and and haematological parameters. Result showed that protein, EE, lysine, methionine and fibre contents increased with increasing levels of CSC in diet, whereas ME in kcal/kg reduced as CSC in diet increased. Feed intake significantly (P<0.05) decreased with increasing levels of CSC in diet across treatment. Final weight gain, average daily live weight and feed to gain ratio significantly (P<0.05) increased when CSC in diet increased from 15% to 45%. Feed cost (N/kg) increased with increased in CSC. However, cost of concentrate intake and feed cost/gain were better for animal fed 45%. Although, varying levels of CSC in diet significantly (P<0.05) influenced the haematological values of WBC, PVC and were within acceptable regions. The distribution of neutrophils, lymptrocytes, monocytes, basophils and eosinophils reduced with increasing levels of CSC in diet, though were not significantly (P>0.05) affected by variation of levels of CSC in diets. It is concluded that including protein supplement such as cottonseed cake in buck diets at 45% can be beneficial to Kano brown bucks.

A 60 day study was carried out to determine the effect of protein supplement in Kano brown bucks. Twenty four bucks with initial weight of 10.25-12.33 kg were grouped into three of eight bucks each and were randomly assigned to the three treatment diets in a completely randomized design. Prior to this, concentrate diets were formulated to contain 15%, 30% and 45% cottonseed cake (CSC) as the protein supplement in the dietary treatments. Crop residues formed the basal diet and were fed ad libitum. Alongside, fresh clean water was provided to goats ad libitum. Parameters determined during the experiment were feed intake, daily weight changes, Feed cost and and haematological parameters. Result showed that protein, EE, lysine, methionine and fibre contents increased with increasing levels of CSC in diet, whereas ME in kcal/kg reduced as CSC in diet increased. Feed intake significantly (P<0.05) decreased with increasing levels of CSC in diet across treatment. Final weight gain, average daily live weight and feed to gain ratio significantly (P<0.05) increased when CSC in diet increased from 15% to 45%. Feed cost (N/kg) increased with increased in CSC. However, cost of concentrate intake and feed cost/gain were better for animal fed 45%. Although, varying levels of CSC in diet significantly (P<0.05) influenced the haematological values of WBC, PVC and were within acceptable regions. The distribution of neutrophils, lymptrocytes, monocytes, basophils and eosinophils reduced with increasing levels of CSC in diet, though were not significantly (P>0.05) affected by variation of levels of CSC in diets. It is concluded that including protein supplement such as cottonseed cake in buck diets at 45% can be beneficial to Kano brown bucks.