Phytochemical Analysis and Antioxidant Property of Leaf Extracts ofVitex donianaandMucuna pruriens

Abstract

Oxidative stress and impaired antioxidant system have been implicated in the pathophysiology of diverse disease states. The phytochemical screening and antioxidant property of fresh leaves of Vitex doniana and Mucuna pruriens, used in the management and treatment of various diseases, were studied. The extracts (ethanol and distilled water) were screened for the presence of phytochemicals, and their inhibition of 2,2-diphenyl-1-picryl-hydrazyl (DPPH) radical was used to evaluate their free radical scavenging activity. Liver levels of malondialdehyde (MDA), superoxide dismutase (SOD), and catalase (CAT) in carbon tetrachloride- (CCl4) treated albino rats were also used to assess the antioxidant activity of the extracts. The animals were treated with 250 mg/kg body weight of the extracts for six consecutive days before a single dose (2.5 mL/kg body weight) of CCl4. Vitamin C was used as the standard antioxidant. Phytochemical screening revealed the presence of saponins, tannins, anthraquinones, terpenoids, and flavonoids in all the extracts, while alkaloids were detected in extracts of Vitex doniana only, and cardiac glycosides occurred in extracts of Mucuna pruriens only. All the extracts inhibited DPPH radical in a concentration-dependent manner, water extract of Vitex doniana producing highest inhibition which was not significantly different (P > .05) from vitamin C. The extracts produced a significant decrease (P < .05) in liver MDA, while the levels of SOD and CAT significantly increased (P < .05) relative to the positive control. These results are an indication of antioxidant potential of the extracts and may be responsible for some of the therapeutic uses of these plants.

Etude de l’activité antioxydante des extraits des feuilles de <i>Vitex doniana</i> (Verbenacea)

“…Les phases hexaniques rassemblées ont été évaporées pour constituer la fraction hexanique. (Agbafor et Nwachukwu, 2011 ;Lagnika et al, 2012).…”

“…Par ailleurs, dans les travaux menés par Agbafor et Nwachukwu (2011) L'objectif du fractionnement bioguidé consistait à identifier une fraction dont l'activité antioxydante est supérieure à celle de l'extrait éthanolique total. Les feuilles de Vitex doniana contiennent des flavonoïdes, des tanins, des terpénoïdes, des composés phénoliques, des alcaloïdes et les vitamines A, C et E (Suleiman et Yusuf, 2008 ;Muanda, 2010 ;James et al, 2014 (Suleiman et Yusuf, 2008 ;Muanda, 2010 ;Lagnika et al, 2012).…”

Screening of Antioxidant Property in Medicinal Plants Belonging to the Family Apocynaceae Zahra

“…Usually, they include steroids, alkaloids, flavonoids, tannins, terpenoids, and several others [2]. Many phytochemicals possess antioxidative property, which can lessen the danger of many diseases [3]. The prominence of therapeutic plants and the impact of phytomedicine derived from it have a significant influence to the well-being of the considerable world population, which have engrossed awareness from different disciplines of research.…”

Acute Toxicity Evaluation of Ethanol Extract of Indonesian Velvet Beans

“…The pod was reported as anthelmintic, while root was used in the treatment of nervous disorders and blood purification. In many countries, this plant is also used in the treatment of cholesterol, diabetes, hypertension, paralysis, rheumatism, cancer, tumors, tuberculosis, asthma, cholera, diarrhea, dysentery, irritation, burns, cough, muscle pain, and many others [5][6][7]. Various studies reported the bioactivity of Mucuna pruriens, such as increasing the sexual activity of normal male rats, hepatoprotective activity with increasing the in vivo antioxidants [8], antidiabetic activity [9][10][11][12], and as a potential antiparkinson from the seeds [13,14].…”

“…The results of the phytochemical study showed that Mucuna pruriens contains saponins, tannins, anthraquinones, steroids, terpenoids, alkaloids, flavonoids, resins, and glycoside-containing compounds [5,[15][16][17]. The seed of Mucuna pruriens is known contains L-DOPA, a precursor to the neurotransmitters dopamine.…”