Acute Toxicity Evaluation of Ethanol Extract of Indonesian Velvet Beans

Sardjono, Ratnaningsih Eko; Musthapa, Iqbal; Sholihin,; Qowiyah, Atun; Rachmawati, Rahmi

doi:10.22159/ijpps.2017v9i5.16284

Cited by 4 publications

(5 citation statements)

References 20 publications

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“…Based on the results obtained, the LD 50 of HAMP after single oral administration to female rats, observed over a period of 14 days, is >2000 mg/kg body weight. Sardjono et al also showed non-toxic effect of MP seed extracts, but it was carried out using ethanol extract, which supports our study [27].…”

Section: Resultssupporting

confidence: 90%

Evaluation of Mucuna Pruriens Seed Extract for Its Acute Oral Toxicity in Albino Rats

Swamy

Rao

Holla

2019

Asian J Pharm Clin Res

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Objectives: The present study was carried out to evaluate the hydroalcoholic extract of Mucuna pruriens (HAMP) seeds for its acute oral toxicity in albino rats. Methods: Acute oral toxicity of MP seed extract was assessed in albino rats with three different doses of the extract with 175, 550, and 2000 mg/ kg body weight. Body weight, mortality, and clinical signs were recorded on 0 (before administration), 7th, and 14th days. Rats were sacrificed after day 14 and observed for any histological changes in the brain, heart, liver, and kidney tissues. Rats were normal up to 1 h and exhibited dullness and piloerection after 1 h which continued up to 2–4 h of observation period on day 0 of administration. All animals appeared normal from day 1 to throughout the experimental procedure. Results: No significant changes in the histological structure of the liver, kidney, and heart were noticed except mild congestion and hydropic changes only in liver tissue seen for 2000 mg/kg body weight of HAMP seeds. The seed extract of MP is non-toxic to rats and did not show any mortality nor the behavioral changes. In addition, it showed an increase in the body weight with the administration up to 2000 mg/kg body weight. Conclusion: MP seed extract signified as neurosuppressant, and the drug can be used in the treatment of neurological disorders characterized by hyperactivity of the neurons. The present data could provide adequate confirmation of the safety of MP for further experimental studies on a standardized formulation of the seeds extract.

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Section: Resultssupporting

confidence: 90%

Evaluation of Mucuna Pruriens Seed Extract for Its Acute Oral Toxicity in Albino Rats

Swamy

Rao

Holla

2019

Asian J Pharm Clin Res

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“…Previous studies showed the ability of SBT to increase blood HDL [14]. Other study results by Sardjono et al [20] showed the ability of velvet bean extract to increase blood HDL. Based on Table 4, VBT and VB-SBT have the potential as alternative foods to increase blood HDL like SBT.…”

Section: Analysis Of Serum Biochemical Profilementioning

confidence: 91%

“…Velvet beans have also been reported to have hypocholesterolemic properties. Several possible reaction mechanisms associated with velvet bean's hypocholesterolemic properties include inhibiting cholesterol biosynthesis in the body, preventing LDL oxidation due to its flavonoid content, converting cholesterol into bile acids, and inhibiting cholesterol absorption in the digestive tract [20].…”

Section: Analysis Of Serum Biochemical Profilementioning

confidence: 99%

“…The ability of VBT and VB-SBT to maintain the value of hematological parameters in rats is inseparable from the role of its constituent minerals. Minerals such as Fe, Mg, Zn, and Cu, as well as vitamin C (ascorbic acid), are known to help the formation of red blood cells [20]. Therefore, integrating velvet beans into the tempe production process can reduce the activity of antinutritional compounds to increase the bioavailability of minerals.…”

Section: Analysis Of the Hematological Profilementioning

confidence: 99%

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The potential of velvet bean tempe to improve hematology and serum biochemical profiles in experimental rats

Damayanti,

Astawan,

Wresdiyati

et al. 2024

IOP Conf. Ser.: Earth Environ. Sci.

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In Indonesia, tempe is the second largest source of protein after rice. The main raw material for tempe is soybean, of which 70% is still obtained through imports. This has encouraged the necessity to explore Indonesia’s indigenous legume products. Velvet bean (Mucuna pruriens L.) contains several bioactive components, is rich in minerals, and is composed of essential and non-essential amino acids, as well as essential and non-essential fatty acids. This study aimed to explore the potential of velvet beans as a substitute ingredient in tempe production. Parameters of serum biochemical profile and blood hematology of rats were used as scientific evidence that can support the purpose of this study. Protein quality was analyzed using the growth method in experimental rats. Three types of tempe were used as protein sources, namely soybean tempe (SBT), velvet bean tempe (VBT), and velvet bean-soybean combination tempe (VB-SBT). Protein quality analysis results show that VB-SBT has FCE, growth rate, and NPR values that were as good (p>0.05) as SBT. Analysis results of serum biochemical and hematological profiles indicate no abnormalities in rats fed with VB-SBT. These results suggest that the substitution of soybean with velvet bean can be applied in the tempe production process.

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“…Subgroup-5 (sodium arsenite + M. pruriens) received sodium arsenite in drinking water (50 mg/L) + M. pruriens at a dose of 350 mg/kg BW [ 22 ] orally once per day for 45 days.…”

Section: Methodsmentioning

confidence: 99%

Ameliorating effect of Mucuna pruriens seed extract on sodium arsenite-induced testicular toxicity and hepato-renal histopathology in rats

Bairy

Raghavendra

2023

Vet World

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Background and Aim: A significant cause of arsenic poisoning is polluted groundwater. Arsenic poisoning results in the suppression of spermatogenesis and the liver and kidneys are vulnerable to the toxic effects as well. Mucuna pruriens has been identified to have fertility-enhancing and anti-lipid peroxidation properties. Based on these properties of M. pruriens, this study aimed to investigate the efficacy of M. pruriens seed extract in reducing sodium arsenite-induced testicular impairment and hepato-renal histopathology in rats. Materials and Methods: The study was divided into two groups; short-term (45 days) and long-term (90 days) treatment groups and each group was divided into nine subgroups. Subgroups 1 and 2 served as normal and N-acetyl cysteine (NAC) controls, respectively. Subgroups 3–9 received sodium arsenite in the drinking water (50 mg/L). Subgroup-4 received NAC (210 mg/kg body weight [BW]) orally once daily. Subgroups 5–7 received aqueous seed extract of M. pruriens (350, 530, and 700 mg/kg BW, respectively) orally once daily. Subgroups 8 and 9 received a combination of NAC and aqueous seed extract (350 and 530 mg/kg BW, respectively) orally once daily. Following the treatment, animals were sacrificed and sperm parameters and DNA damage were evaluated. Testis, liver, and kidneys were analyzed for histopathology. Results: Sodium arsenite-induced a significant reduction in sperm parameters and increase in the abnormal architecture of spermatozoa. Histology revealed tissue necrosis. The M. pruriens seed extract ameliorated the damaging effects of sodium arsenite with respect to tissue architecture and sperm parameters when coadministered. Conclusion: Mucuna pruriens has beneficial effects against the deleterious effects of sodium arsenite on various tissues. Thus, M. pruriens (530 and 700 mg/kg BW) supplementation would reduce the adverse changes observed with sodium arsenite exposure.

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Acute Toxicity Evaluation of Ethanol Extract of Indonesian Velvet Beans

Cited by 4 publications

References 20 publications

Evaluation of Mucuna Pruriens Seed Extract for Its Acute Oral Toxicity in Albino Rats

Evaluation of Mucuna Pruriens Seed Extract for Its Acute Oral Toxicity in Albino Rats

The potential of velvet bean tempe to improve hematology and serum biochemical profiles in experimental rats

Ameliorating effect of Mucuna pruriens seed extract on sodium arsenite-induced testicular toxicity and hepato-renal histopathology in rats

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