Phytochemical analyses and pharmacological screening of Neem oil

Cesa, Stefania; Sisto, Francesca; Zengin, Gökhan; Scaccabarozzi, Diletta; Kokolakis, Antonios K.; Scaltrito, M.M.; Grande, Rossella; Locatelli, Marcello; Cacciagrano, Francesco; Angiolella, Letizia; Campestre, Cristina; Granese, Arianna; Chimenti, Paola; Basilico, Nicoletta

doi:10.1016/j.sajb.2018.10.019

Cited by 31 publications

(22 citation statements)

References 42 publications

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“…To evaluate the color properties of hemp seed oils, the tristimulus colorimetry was employed. To our knowledge, only one work is available in literature regarding colorimetric studies on hemp seed oil samples, reporting an L* value of 18.13, an a* of 2.40 and a b* of 30.87 [46], so that a significant yellow parameter (+b*) can be shown, compared to a very weak red value (+a*) and a quite dark sample.…”

Section: Colorimetrymentioning

confidence: 99%

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Commercial Hemp Seed Oils: A Multimethodological Characterization

et al. 2020

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Nine commercial hemp seed oils from different countries were studied using a multimethodological approach to obtain information about their quality and chemical composition. Due to the lack of a specific regulation for hemp seed oils, quality parameters used in the case of olive oils (free acidity, peroxides number, spectrophotometer parameters) and anisidine number were measured and compared with those reported for extra virgin olive oil (EVOO). Free acidity and peroxides number showed a great variability, ranging from 0.4 to 17.24% and from 4.32 to 22.14 meqO2/kg, respectively, whereas the anisidine number ranged from 0.11 to 3.58. K232 value turned out to be generally below the limit reported for EVOO, whereas K270 and ΔK values were higher, with respect to EVOO limits, due to the high amount of tri-unsaturated fatty chains. Colorimetric analysis showed a peculiar curve trend that could represent the fingerprint of this product. Untargeted nuclear magnetic resonance methodology allowed to measure the amount of fatty chains, ω-6:ω-3 ratio, β-sitosterol, and aldehydes. The ω-6:ω-3 ratio turned out to be, in some cases, different from that reported on the bottle labels. Finally, lipoperoxidation assays were also carried out under different storage (light and temperature) and time exposure conditions, confirming that the exposure to direct light is the condition that interferes more with the product quality.

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Section: Colorimetrymentioning

confidence: 99%

“…In a previous work [46], a comparison among medium reflectance profiles of hemp seed, olive, and neem oils has been carried out: a similarity among olive oils and hemp oils has been observed, although hemp seed oils turned out to be darker.…”

Section: Colorimetrymentioning

confidence: 99%

Commercial Hemp Seed Oils: A Multimethodological Characterization

et al. 2020

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“…Neem oil has been used since ancient times in the popular medicine thanks to its wide spectrum of biological activities such as antioxidant, antinflammatory, antibacterial [ 3 ], antifungal and antiparasitic [ 4 , 5 ]. It is obtained from the neem tree belonging to the family of the Meliaceae ( Azadirachta indica A.Juss.)…”

Section: Introductionmentioning

confidence: 99%

“…Some studies reported antitumor and chemo-preventive effects of neem oil in different types of cancer thanks to its ability to balance redox reactions and to stimulate the host immune responses [ 10 ]. Further, in traditional Indian medicine it is used for its moisturizing, antiaging and regenerative properties, mainly connected with its lipophilic composition, fatty acid content (e.g., oleic, palmitic and stearic acids) and antioxidant potential [ 4 ]. It is considered a convenient and effective plant-derived oil for wound healing in animals [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Nanotechnology for Natural Medicine: Formulation of Neem Oil Loaded Phospholipid Vesicles Modified with Argan Oil as a Strategy to Protect the Skin from Oxidative Stress and Promote Wound Healing

et al. 2021

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Neem oil, a plant-derived product rich in bioactives, has been incorporated in liposomes and hyalurosomes modified by adding argan oil and so called argan-liposomes and argan-hyalurosomes. Argan oil has also been added to the vesicles because of its regenerative and protective effects on skin. In the light of this, vesicles were specifically tailored to protect the skin from oxidative stress and treat lesions. Argan-liposomes were the smallest vesicles (~113 nm); the addition of sodium hyaluronate led to an increase in vesicle size (~143 nm) but it significantly improved vesicle stability during storage. In vitro studies confirmed the free radical scavenging activity of formulations, irrespective of their composition. Moreover, rheological investigation confirmed the higher viscosity of argan-hyalurosomes, which avoid formulation leakage after application. In vitro studies performed by using the most representative cells of the skin (i.e., keratinocytes and fibroblasts) underlined the ability of vesicles, especially argan-liposomes and argan-hyalurosomes, to counteract oxidative stress induced in these cells by using hydrogen peroxide and to improve the proliferation and migration of cells ensuring the more rapid and even complete closure of the wound (scratch assay).

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“…However, several issues do exist in the discovery of the novel drugs, such as toxicity, drug selectivity, and smart sensing of target. Plant derived metabolites are used as traditional medicine to cure several diseases because of their remarkable properties, such as anti-inflammatory, antimicrobial, antidiabetics, antioxidants, anticarcinogenic, antidepression, antiobesity, immunostimulatory, and antianxiety [6,7,8,9]. These health beneficial properties of plant-derived metabolites are due to the existence of novel flavonoids, simple phenolics, anthocyanins, and phenolic acids [7,10].…”

Section: Introductionmentioning

confidence: 99%

Antioxidant, Anti-Lung Cancer, and Anti-Bacterial Activities of Toxicodendron vernicifluum

Saravanakumar

Chelliah

et al. 2019

Biomolecules

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This work tested antioxidant, anti-lung cancer, and antibacterial activities by in vitro, in vivo, and computational experiments for the metabolites extracted from the bark, seed, and stem of Toxicodendron vernicifluum. The results showed that all the extracts significantly scavenged 1,2-diphenyl-1-picrylhydrazyl (DPPH) in a dose-dependent manner. But, the total phenol content (TPC) ranged from 2.12 to 89.25% and total flavonoids content (TFC) ranged from 1.02 to 15.62% in the extracts. The methanolic bark extract (MBE) exhibited higher DPPH scavenging activity than the other extracts, probably due to the higher content of the TPC and TFC present in it. Among the extracts, only the MBE showed anti-lung cancer activity at an acceptable level with a therapeutic index value (22.26) against human lung carcinoma. This was due to the cancer cell death in A549 induced by MBE through reactive oxygen species (ROS) generation, apoptosis, and cell arrest in G1 phase and inhibition of anti-pro-apoptotic protein survivin. Among the extracts, MBE showed significantly higher antibacterial activity as evident through the higher zone of inhibition 13 ± 0.5 mm against methycilin resistant strain of Staphylococcus aureus (MRSA), Salmonila enteria subp. enterica, and P. aeruginosa, 11 ± 0.3 mm against E. coli and 10 ± 0.2 mm against B. cereus. The MBE also showed an excellent antibacterial activity with lower minimal inhibitory concentration (MIC). Particularly, the MBE showed more significant antibacterial activity in MRSA. The in vivo antibacterial activity of the MBE was further tested in C. elegans model. The treatment of the MRSA induced cell disruption, damage and increased mortality of C. elegans as compared to the untreated and MBE treated C. elegans with normal OP50 diet. Moreover, the MBE treatment enhanced the survival of the MRSA infected C. elegans. The compounds, such as 2,3,3-trimethyl-Octane and benzoic from the MBE, metabolized the novel bacterial topoisomerases inhibitor (NBTI) and MRSA related protein (PBP2a). Overall the T. vernicifluum is potentially bioactive as evident by antioxidant, anti-lung cancer, and antibacterial assays. Further studies were targeted on the purification of the novel compounds for the clinical evaluation.

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Phytochemical analyses and pharmacological screening of Neem oil

Cited by 31 publications

References 42 publications

Commercial Hemp Seed Oils: A Multimethodological Characterization

Commercial Hemp Seed Oils: A Multimethodological Characterization

Nanotechnology for Natural Medicine: Formulation of Neem Oil Loaded Phospholipid Vesicles Modified with Argan Oil as a Strategy to Protect the Skin from Oxidative Stress and Promote Wound Healing

Antioxidant, Anti-Lung Cancer, and Anti-Bacterial Activities of Toxicodendron vernicifluum

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