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Background: Biogenic silver nanoparticles (AgNPs) have wider range of biomedical applications. The present work synthesized Tp-AgNPs using mycelial extract of endophytic fungus Talaromyces purpureogenus (MEEF), characterized, and analyzed for antibacterial, anti-proliferation and cell wounding healing activities. Methods: The synthesized Tp-AgNPs were characterized by UV-visible spectrophotometer (UV-Vis), field emission transmission electron microscopy (FETEM) with energy-dispersive X-ray spectroscopy (EDS), Fourier transform infrared spectroscopy (FTIR), particle size analysis (PSA) and X-ray diffraction (XRD). Further, antibacterial activity was determined by Kirby–Bauer test and anti-proliferation activity was tested in human lung carcinoma A549 by water-soluble tetrazolium and flow cytometer assay. In addition, cell wounding healing activity was determined by scratch assay. Results: UV-Vis results displayed a strong absorption peak from 390 nm to 420 nm, which indicated the successful synthesis of Tp-AgNPs. FETEM-EDS results indicated the round and triangle shaped Tp-AgNPs with the average size of 25 nm in accordance with PSA. FTIR analysis indicated the involvement of various functional molecules from MEEF in the synthesis of Tp-AgNPs. XRD result proved nature of Tp-AgNPs as a high-quality crystal. The Tp-AgNPs significantly inhibited the growth of bacterial pathogens at the minimal inhibitory concentration of 16.12 μg.mL −1 for Gram + , and 13.98 μg.mL −1 for Gram − bacteria. Further, Tp-AgNPs (2 μg.mL −1 ) showed a strong anti-proliferation effect in A549. Interestingly, Tp-AgNPs was not cytotoxic to normal NIH3T3 cells. In addition, the NPs exhibited a strong cell wounding healing activity. Conclusion: This work biosynthesized AgNPs with strong antibacterial, anticancer and cell wound healing properties using endophytic fungus T. purpureogenus .

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The clean and eco-friendly synthesis of silver nanoparticles (AgNPs) has provided promising characteristics with impressive biomedical related potential. Here, we have employed a green process for the synthesis of AgNPs using kenaf seed (KS) extract as a bilateral mediator for reducing and capping of Ag + ions under hydrothermal condition. The synthesis pathways, such as varying amounts of KS, Ag ion concentration and autoclaving time were optimized. The manifestation of a strong absorption peak from 420-430 nm in UV-vis spectroscopy indicated the successful synthesis of KS@AgNPs. Fourier transform infrared spectroscopy confirmed the presence of hydroxyl and carbonyl functionalities involved in the reduction and stabilization of Ag + ions. Furthermore, transmission electron microscopy revealed that the KS@AgNPs are spherical in shape having a size around 7-11 nm, whereas high-quality crystals were evidenced by x-ray diffraction analysis. Moreover, inductively coupled plasma-optical emission spectrometry revealed that 19.6 µg l −1 of Ag + ions were released from the KS@AgNPs. In cell line studies, KS@AgNPs at a higher dose were shown to be non-toxic to the healthy (NIH3T3) cells, while strong anti-proliferative response was found in the case of lung cancer (A549) cells. Furthermore, a significant zone of inhibition was observed for both Gram-positive and Gram-negative microorganisms, and a combination of KS@AgNPs with ampicillin revealed a notable synergistic anti-pathogenic effect. Overall, our study proved the potentiality of KS as an efficient bio-resource for the synthesis of AgNPs and also its original feature as an anti-cancer and antimicrobial agent.

Objective: In this study, we aimed to clarify the effects of long noncoding ribonucleic acid prostrate androgen-regulated transcript-1 on bladder cancer cell proliferation and apoptosis. Methods: Microarrays were implemented to investigate the long noncoding ribonucleic acid expression profiles in bladder cancer tissue (N = 9) and in noncancer bladder tissue (N = 5). Relative prostrate androgen-regulated transcript-1 expression levels in tissue samples or cell lines were detected by real-time quantitative reverse transcription-polymerase chain reaction. Prostrate androgen-regulated transcript-1 expression was enhanced by the transfection of pcDNA3.1-prostrate androgen-regulated transcript-1 and downregulated by the infection with pcMV-sh prostrate androgen-regulated transcript-1. Additionally, cell proliferation and apoptosis were measured by the cell counting kit-8 assay and flow cytometry, respectively. Cell invasion was determined by a Transwell assay. Results: Prostrate androgen-regulated transcript-1 expression was upregulated in bladder cancer tissues compared to adjacent nontumor tissues. Furthermore, prostrate androgen-regulated transcript-1 levels were successfully upregulated by pcDNA3.1-prostrate androgen-regulated transcript-1 and depleted by pCMV-sh prostrate androgen-regulated transcript-1 in bladder cancer cell lines (5637, T24). Enhanced prostrate androgen-regulated transcript-1 expression promoted cell proliferation and invasion and inhibited cell apoptosis. However, knockdown of prostrate androgen-regulated transcript-1 expression inhibited cell proliferation and invasion and induced cell apoptosis. Conclusion: In summary, these data suggest that the knockdown of prostrate androgen-regulated transcript-1 represents a tumor suppressor player in bladder cancer and contributes to the inhibition of tumor proliferation, the promotion of cell apoptosis, and the suppression of cell invasion. Prostrate androgen-regulated transcript-1 may function as a new prognostic biomarker and as a feasible therapeutic target for patients with bladder cancer.

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