PHYTO-PHARMACOGNOSTICAL EVALUATION AND HPTLC STUDY ON ANANTAMUL (HEMIDESMUS INDICUS R.Br.) ROOT

Das, Manosi; Banerjii, Avijit; Dutta, Sreya; Saha, Sanjoy Kumar; Mondal, D. N.; Hazra, Jayram

doi:10.7897/2277-4343.083146

Cited by 4 publications

(2 citation statements)

References 1 publication

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“…Phalsa pomace's flavonoid concentration was estimated to be 12.42056 CE mg/g [36,37]; it was found that the rind/bark contained 39.114 ± 65 mg of flavonoids. The results of another investigation showed that the fruit's solvent extract's total flavonoid concentration and phenolic content were, respectively, 0.13 and 5.25 GAE/mL [38]. The bioactive potential of various parts of berry trees is given in detail in Table 2.…”

Section: Flavonoidsmentioning

confidence: 99%

A Review of Emerging Technologies for the Extraction of Bioactive Compounds from Berries (Phalsa Berries)

Shams,

Kaur,

Dash

et al. 2024

Horticulturae

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Berries have been gaining in popularity among consumers and producers due to their natural bioactive compounds that have beneficial effects on human health. This review aimed to identify effective techniques for the extraction of bioactive compounds from berries, consolidate the findings of recent studies using various extraction technologies, and provide a global perspective on the research trends in this field. These extraction techniques include pulsed electric field, ultrasound-assisted extraction, pressurized liquid extraction, microwave-assisted extraction, and supercritical CO2 extraction. The solid waste generated during the industrial berry juice production process is assumed to be a less expensive source of raw materials for the natural extraction of bioactive compounds. The main aim of modern techniques is to produce more of the desired compound and find a method to extract bioactive compounds from berries without the use of hazardous solvents. These include flavonoids, phenols, anthocyanins, and antioxidants. Regarding the characterization of the bioactive compounds that are isolated from berries, aspects such as scanning electron microscopy, X-ray powder diffraction, Fourier transform infrared spectroscopy, and nuclear magnetic resonance were reviewed.

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Section: Flavonoidsmentioning

confidence: 99%

A Review of Emerging Technologies for the Extraction of Bioactive Compounds from Berries (Phalsa Berries)

Shams,

Kaur,

Dash

et al. 2024

Horticulturae

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“…The use of herbal drugs is rapidly increasing worldwide as the herbal drugs were found to be beneficial in treating mild to moderate diseases in all age groups and in averting illnesses thereby promoting health (2). Roots of H. indicus is an important plant drug used as a tonic, demulcent, diaphoretic and diuretic as per Siddha literature, it is used to treat a variety of diseases such as leprosy, leucoderma, itching, skin disease, body coolant, asthma, bronchitis, leucorrhoea, dysentery, piles, syphilis, paralysis, urinary disorders and diabetes (3). Hemidesmus indicus (L.) R. Br.…”

Section: Introductionmentioning

confidence: 99%

Detection of adulteration of Decalepis hamiltonii Wight & Arn. with Hemidesmus indicus (L.) R. Br. by pharmacognostic, molecular DNA fingerprinting by RAPD, chemical and HPTLC studies

et al. 2021

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Hemidesmus indicus (L.) R. Br. (Apocynaceae) root is extensively used in Indian traditional systems due to its biological activities. Decalepis hemiltonii Wight & Arn. is another member from the same family resembling H. indicus and is adulterated in the herbal market. Aim of the study was to compare and evaluate the distinguishing features based on macroscopy, microscopy, powder microscopy, molecular differences in the genomic DNA by RAPD, physiochemical, phytochemical screening, TLC and HPTLC fingerprint profiling of successive extracts. Microscopically cork, cortex, phloem, xylem, medullary rays and pith; powder microscopically size and shape of the cork cells, fibre, fibre tracheids, vessels, xylem parenchyma cells were different from each other. Polymorphism (75.4 %) was found in eight primers out of 16 primers analyzed. The water soluble extractive and the hexane soluble extractive of D. hamiltonii was higher than H. indicus. Tannins, flavonoids, steroids and coumarins were present only in H. indicus and absent in D. hamiltonii. After derivatization, spots at Rf 0.88 (hexane extract), 0.81 (chloroform extract) and 0.55 (ethanol extract) in H. indicus; spots at Rf 0.22, 0.45 (chloroform extract), 0.19, 0.35, 0.58, 0.59 (ethanol extract) in D. hamiltonii were observed. This study will be helpful to find out adulteration of D. hemiltonii in place of H. indicus sold in the crude drug market and in herbal formulations.

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Box–Behnken design avenue for development and validation of high‐performance thin‐layer chromatography method for estimation of rutin in Hemidesmus indicus

Shrivastava

Daharwal

2021

Biomedical Chromatography

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The aim of this study was to determine the content of rutin in Hemidesmus indicus and to optimize the high‐performance thin‐layer chromatography method. The method was validated in compliance with the International Council for Harmonisation guidelines Q2 (R1) for parameters such as linearity, accuracy, precision, robustness, limit of detection, and limit of quantitation. A Box–Behnken design and response surface methodology has been used to investigate the impact of independent variables on the response. Three independent variables, mobile phase composition (% v/v), mobile phase volume (mL), and duration of saturation (min), were studied. Rutin was verified, and its content was determined using a validated high‐performance thin‐layer chromatography method with good linearity within the range of 200–1000 ng spot–1 with r2 = 0.9998 and correlation coefficient with calibration curve equation y = 0.0297x + 0.0001. The average percentage recovery values varied from 99.03 to 101.15 and 98.88 to 100.12%, respectively, for in‐house and marketed mother tincture). The peak area determination at three different concentration levels shows low values of percentage relative standard deviation (<2%) for inter‐day (0.04–0.06) and intra‐day (0.04–0.05) precision of rutin. The average content of rutin in extract and marketed mother tincture was 229 ± 0.57 and 210 ± 0.57 μg g–1. The proposed method was simple, precise, and accurate for the determination of rutin with frequent quality control assessment of H. indicus.

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PHYTO-PHARMACOGNOSTICAL EVALUATION AND HPTLC STUDY ON ANANTAMUL (HEMIDESMUS INDICUS R.Br.) ROOT

Cited by 4 publications

References 1 publication

A Review of Emerging Technologies for the Extraction of Bioactive Compounds from Berries (Phalsa Berries)

A Review of Emerging Technologies for the Extraction of Bioactive Compounds from Berries (Phalsa Berries)

Detection of adulteration of Decalepis hamiltonii Wight & Arn. with Hemidesmus indicus (L.) R. Br. by pharmacognostic, molecular DNA fingerprinting by RAPD, chemical and HPTLC studies

Box–Behnken design avenue for development and validation of high‐performance thin‐layer chromatography method for estimation of rutin in Hemidesmus indicus

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