Physiological performance, secondary metabolite and expression profiling of genes associated with drought tolerance in Withania somnifera

Sanchita, .; Singh, Ruchi; Mishra, Anand; Dhawan, Sunita Singh; Shirke, Pramod Arvind; Gupta, Madan M.; Sharma, Ashok

doi:10.1007/s00709-015-0771-z

Cited by 38 publications

(17 citation statements)

References 66 publications

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“…All the genes considered in this study were transparent testa glabra 1 (TTG1), transcription factor, STICHEL, and trihelix DNA-binding protein (GTL1), genes responsible for trichome development and its formation, which showed 6, 8.2, and 7 times increased expression, respectively, in O. kilimandscharicum, whereas 0.5, 1.5, and 0.1, respectively, in O. basilicum and 1, 7.5, and 0.2 upregulated expression, respectively, in comparison to their hybrid (Sanchita et al 2015). Microspore-specific promoter 2, a cold responsible gene, has shown 2.2, 7.5, and 0 times increased expression, respectively, in O. kilimandscharicum, hybrid and O. basilicum genotypes and in the case of the WRKY transcription factor gene, shows down-regulation with factor −0.5, −2, and 0 times overexpressions, respectively.…”

Section: Gene Expression Analysis From Leaf Tissues Of O Basilicum mentioning

confidence: 96%

“…This reaction mix was dispensed in a 96-well PCR plate in triplicates. The thermal profile of the reaction was an initial denaturation at 95°C for 2 min, followed by 40 cycles at 95°C for 10 s and 60°C for 10 s. This was followed by fluorescence acquisition after each cycle (Sanchita et al 2015). Baseline and Ct values were automatically calculated by the Sequence Detection Systems (SDS) software version 2.2.1 (Applied Biosystems).…”

Section: Quantitative Real-time Pcrmentioning

confidence: 99%

“…An increased proline content at the biochemical level represents a higher potential to survive in a stress condition like cold stress as reported in different plant species, therefore, proline was also estimated in all the three genotypes. Many genes and transcription factors are involved in the formation and regulation of trichome development and adaptation towards various stresses, thus involved in many physiological processes (Sanchita et al 2015). We have selected some of the key factors and analyzed them in the parent species and cold-tolerant evergreen hybrid for their expression.…”

Section: Introductionmentioning

confidence: 99%

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A cold-tolerant evergreen interspecific hybrid of Ocimum kilimandscharicum and Ocimum basilicum: analyzing trichomes and molecular variations

et al. 2015

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Ocimum (Lamiaceae) is an important source of essential oils and aroma chemicals especially eugenol, methyl eugenol, linalool, methyl chavicol etc. An elite evergreen hybrid has been developed from Ocimum kilimandscharicum and Ocimum basilicum, which demonstrated adaptive behavior towards cold stress. A comparative molecular analysis has been done through RAPD, AFLP, and ISSR among O. basilicum and O. kilimandscharicum and their evergreen cold-tolerant hybrid. The RAPD and AFLP analyses demonstrated similar results, i.e., the hybrid of O. basilicum and O. kilimandscharicum shares the same cluster with O. kilimandscharicum, while O. basilicum behaves as an outgroup, whereas in ISSR analysis, the hybrid genotype grouped in the same cluster with O. basilicum. Ocimum genotypes were analyzed and compared for their trichome density. There were distinct differences on morphology, distribution, and structure between the two kinds of trichomes, i.e., glandular and non-glandular. Glandular trichomes contain essential oils, polyphenols, flavonoids, and acid polysaccharides. Hair-like trichomes, i.e., non-glandular trichomes, help in keeping the frost away from the living surface cells. O. basilicum showed less number of non-glandular trichomes on leaves compared to O. kilimandscharicum and the evergreen cold-tolerant hybrid. Trichomes were analyzed in O. kilimandscharicum, O. basilicum, and their hybrid. An increased proline content at the biochemical level represents a higher potential to survive in a stress condition like cold stress. In our analysis, the proline content is quite higher in tolerant variety O. kilimandscharicum, low in susceptible variety O. basilicum, and intermediate in the hybrid. Gene expression analysis was done in O. basilicum, O. kilimandscharicum and their hybrid for TTG1, GTL1, and STICHEL gene locus which regulates trichome development and its formation and transcription factors WRKY and MPS involved in the regulation of plant responses to freezing and cold. The analysis showed that O. kilimandscharicum and the hybrid were very close to each other but O. basilicum was more distinct in all respects. The overexpression of the WRKY coding gene showed high expression in the hybrid as compared to O. kilimandscharicum and O. basilicum and the transcription factor microspore-specific (MPS) promoter has also shown overexpression in the hybrid for its response against cold stress. The developed evergreen interspecific hybrid may thus provide a base to various industries which are dependent upon the bioactive constituents of Ocimum species.

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Section: Gene Expression Analysis From Leaf Tissues Of O Basilicum mentioning

confidence: 96%

Section: Quantitative Real-time Pcrmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A cold-tolerant evergreen interspecific hybrid of Ocimum kilimandscharicum and Ocimum basilicum: analyzing trichomes and molecular variations

et al. 2015

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“…Similarly, the leaf length and area of the Axonopus compressus were also affected by limited water supply. Drought stress promotes un-stabilization of the plasma membrane, decreases cell turgor, thus cell damage occurs with the ultimate decrease in the photosynthetic activity and falsifies the light-capturing apparatus, phenomena that largely related to drought stress [58]. Drought also triggered ROS (mainly H 2 O 2 )…”

Section: Discussionmentioning

confidence: 99%

Protection Mehcanism Against Drought In Axsonopus Compressus: Insight of Physio-Biochemical Traits, Antioxident Interplay and Gene Experssion

Nawaz¹,

Liao²,

Azeem³

et al. 2020

Preprint

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Drought is a major environmental constraint that affects plant growth and internal physio-biochemical features. The present study was conducted to evaluate the performance of three different Axonopus compressus accessions, i.e., A-38, A-58, and A-59 under well-watered (WW), low drought (LD), moderate (MD) and severe drought (SD) conditions at field capacity of 100, 80, 60, and 40%, respectively. Results indicated that drought-induced higher production of proline and soluble sugar (SS) up to 40 and 41% respectively, than control. Drought stress caused excessive production of H 2 O 2 while the highest value (10.15µmol g -1 FW) was observed in the A-38 under SD. However, the lowest enzymatic (SOD, POD, CAT, and APX) activity were observed in A-38 than A-58 and A-59 respectively, in the SD. In A-58 the efficient enzymatic and nonenzymatic defense systems hinder the severe damage while stunted growth occurred in Axonopus compressus accessions at SD which were more pronounced in the A-38. Overall, the performance of all Axonopus compressus accessions under drought stress was recorded as A-58>A-59>A-38. The qRT PCR expression analysis also revealed highest expression of drought responsive genes in A-58 and reinforced the findings of physiological data. These results suggested the plant's ability to maintain its functions during drought induction could be used for further investigation under scarce water for developing drought tolerance.

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“…Secondary metabolites such as phenols, flavonoids, and tannins play a pivotal role in the adaptation and defense of plants against adverse environmental conditions such as drought (Sanchita et al, 2015). Synthesis of secondary metabolites in plants during drought stress will help in signaling, and coping up with the over reduced environment generated in the plant cell due to imbalance in the photosynthesis (Alessandra et al, 2019).…”

Section: Total Phenols Flavonoids and Tanninsmentioning

confidence: 99%

Changes in the antioxidant intensities of seven different soybean ( Glycine max (L.) Merr.) cultivars during drought

Rao

Chaitanya

2019

J Food Biochem

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Drought stress-induced antioxidative defense responses were studied in seven soybean (Glycine max (L) Merr.) cultivars PUSA 9712, LSB 1, JS 335, ADB 22, NRC 37, DSB 20, and MAUS 61, respectively. Drought stress was imposed by withholding irrigation till the leaf water potentials reached −1.0, −1.5, and −2.0 MPa. Lipid peroxidation and superoxide, H 2 O 2 , • OH, DPPH radical scavenging assays were performed. Antioxidants during drought stress were studied by quantifying the ascorbic acid, glutathione, α-tocopherol, phenolics, flavonoids, tannins, enzymes superoxide dismutase, catalase, ascorbate peroxidase, and glutathione reductase. Antioxidant levels were significantly high in soybean foliages exposed to drought with the increased concentrations of radicals and enhanced lipid peroxidation rates. Isoenzyme analysis of SOD has shown the upregulation of Cu/Zn and MnSODs. Our results demonstrate that cultivars PUSA 9712 and LSB1 have shown to possess a high rate of antioxidants and a very efficient antioxidative defense system when compared with other cultivars. Practical applicationsSoybean (Glycine max (L.) Merr.) is an economically important cash crop, cultivated globally for its rich protein, oil, nutraceutical, and antioxidant constituents. It is also cultivated for its rich enriched food, animal feed as well as several other commercial products. In India, most of the soybean cultivation is being done under rainfed conditions. Hence, identification of superior soybean cultivars rich in antioxidants is very important. The work presented here is the output of the investigation carried out on cramming the changes in antioxidative defense mechanisms of seven popular soybean cultivars that are regularly cultivated in India. A study was performed by quantifying the radicals, H 2 O 2 concentrations, lipid peroxidation, determination of enzymatic, and nonenzymatic antioxidants in all selected soybean cultivars exposed to control and increased the duration of drought stress, for the identification of superior cultivars with a rich avenue of enzymatic and nonenzymatic antioxidants. K E Y W O R D S antioxidants, drought stress, ROS, secondary metabolites, soybean cultivars How to cite this article: Easwar Rao D, Viswanatha Chaitanya K. Changes in the antioxidant intensities of seven different soybean (Glycine max (L.) Merr.) cultivars during drought.

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Physiological performance, secondary metabolite and expression profiling of genes associated with drought tolerance in Withania somnifera

Cited by 38 publications

References 66 publications

A cold-tolerant evergreen interspecific hybrid of Ocimum kilimandscharicum and Ocimum basilicum: analyzing trichomes and molecular variations

A cold-tolerant evergreen interspecific hybrid of Ocimum kilimandscharicum and Ocimum basilicum: analyzing trichomes and molecular variations

Protection Mehcanism Against Drought In Axsonopus Compressus: Insight of Physio-Biochemical Traits, Antioxident Interplay and Gene Experssion

Changes in the antioxidant intensities of seven different soybean ( Glycine max (L.) Merr.) cultivars during drought

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