Changes in the antioxidant intensities of seven different soybean ( Glycine max (L.) Merr.) cultivars during drought

Abstract

Drought stress-induced antioxidative defense responses were studied in seven soybean (Glycine max (L) Merr.) cultivars PUSA 9712, LSB 1, JS 335, ADB 22, NRC 37, DSB 20, and MAUS 61, respectively. Drought stress was imposed by withholding irrigation till the leaf water potentials reached −1.0, −1.5, and −2.0 MPa. Lipid peroxidation and superoxide, H 2 O 2 , • OH, DPPH radical scavenging assays were performed. Antioxidants during drought stress were studied by quantifying the ascorbic acid, glutathione, α-tocopherol, phenolics, flavonoids, tannins, enzymes superoxide dismutase, catalase, ascorbate peroxidase, and glutathione reductase. Antioxidant levels were significantly high in soybean foliages exposed to drought with the increased concentrations of radicals and enhanced lipid peroxidation rates. Isoenzyme analysis of SOD has shown the upregulation of Cu/Zn and MnSODs. Our results demonstrate that cultivars PUSA 9712 and LSB1 have shown to possess a high rate of antioxidants and a very efficient antioxidative defense system when compared with other cultivars. Practical applicationsSoybean (Glycine max (L.) Merr.) is an economically important cash crop, cultivated globally for its rich protein, oil, nutraceutical, and antioxidant constituents. It is also cultivated for its rich enriched food, animal feed as well as several other commercial products. In India, most of the soybean cultivation is being done under rainfed conditions. Hence, identification of superior soybean cultivars rich in antioxidants is very important. The work presented here is the output of the investigation carried out on cramming the changes in antioxidative defense mechanisms of seven popular soybean cultivars that are regularly cultivated in India. A study was performed by quantifying the radicals, H 2 O 2 concentrations, lipid peroxidation, determination of enzymatic, and nonenzymatic antioxidants in all selected soybean cultivars exposed to control and increased the duration of drought stress, for the identification of superior cultivars with a rich avenue of enzymatic and nonenzymatic antioxidants. K E Y W O R D S antioxidants, drought stress, ROS, secondary metabolites, soybean cultivars How to cite this article: Easwar Rao D, Viswanatha Chaitanya K. Changes in the antioxidant intensities of seven different soybean (Glycine max (L.) Merr.) cultivars during drought.

Natural immunity stimulation using ELICE16INDURES® plant conditioner in field culture of soybean

“…It is also hypothesized that H 2 O 2 is likely to play a key role in preventing biotic stressor-induced infections [ 61 ]. As a result of the previous effects, the gene expression level of the ascorbate peroxidase (APX) enzyme also increased, which can also indirectly explain the quantitative increase in H 2 O 2 production [ 62 ]. In plants, APXs can neutralize the accumulated H 2 O 2 by converting it to water [ 63 ].…”

Nuclear transport factor GmNTF2B‐1 enhances soybean drought tolerance by interacting with oxidoreductase GmOXR17 to reduce reactive oxygen species content

“…, 2018). The ASC and GSH cycle system is one of the keys in the process of ROS scavenging (Easwar Rao and Viswanatha Chaitanya, 2020). To determine whether the molecules responsible for ROS scavenging are depleted when ROS concentration in the nucleus is overexpressed, the oxidation levels of ASC‐GSH and lipids in the cells were measured (Figure 7).…”

Molecular mechanisms of flavonoid accumulation in germinating common bean (Phaseolus vulgaris) under salt stress

“…However, flavonoids, including rutin, are stressful substances, and prolonged stress does not necessarily enable continued flavonoid accumulation ( 57 ). The antioxidant levels in soybean during drought showed an alternating increasing and decreasing trend, suggesting that short-term drought could rapidly enhance antioxidant intensities, while long-term droughts might cause plants to gradually adapt to these stresses and stop enhancement ( 58 ). Also, plant antioxidant enzyme activities showed a similar trend under abiotic stress, which revealed that short-term stress could increase plants' secondary metabolite production, while secondary metabolites under long-term stress might return to a lower level ( 59 ).…”