Physiological and Tolerance Indices Useful for Drought Tolerance Selection in Smooth Bromegrass

Saeidnia, Fatemeh; Majidi, Mohammad Mahdi; Mirlohi, Aghafakhr; Soltan, Saba

doi:10.2135/cropsci2016.07.0636

Cited by 17 publications

(11 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Compared with autoclaved medium, the activities of the antioxidant enzymes POX, CAT and SOD increased in seedlings cultured on media with ≤29.3 μM CD, and decreased significantly in media sterilized with 88.0 μM CD ( P < 0.05). This is in accordance to the physiological responses of plants to other environmental stressors, such as hydrogen peroxide 33 , lower temperature 34 and drought 35 . MDA, one of the end products of stress-induced lipoperoxidation of polyunsaturated fatty acids, is an important index that can indicate the function and integrity of the plant cell membrane 36–38 .…”

Section: Discussionsupporting

confidence: 83%

Use of chlorine dioxide to sterilize medium for tissue culture of potato

Duan

Zhang

Sun

et al. 2019

Sci Rep

View full text Add to dashboard Cite

In vitro cultured seedlings or microtubers are the major starting materials for the production of potato. Currently, seedlings are cultured in media sterilized by autoclaving, which, however, consumes more electricity and takes longer for sterilization, and also requires high temperature-tolerant vessel materials. In order to identify alternative methods of sterilizing culture conditions, the disinfection effects of chlorine dioxide (CD) at 88.0, 29.3, 17.6, 12.6 and 8.8 μM were evaluated in potato medium and vessels. The ≥12.6 μM gaseous CD effectively disinfected vessel through a 30-min fumigation process, and its aqueous solution disinfected potato medium efficiently as well. In presence of 12.6 μM CD in the medium, the potato seedlings had similar morphological features as those grown on autoclaved medium, with some exceptions. The use of 12.6–29.3 μM aqueous CD to sterilize the medium increased antioxidant enzyme activities in potato seedlings, while the use of higher concentration decreased antioxidant enzyme activity levels. SSR analysis did not reveal significant molecular differences in potato seedlings cultured between autoclaved and CD-sterilized medium. In addition to this, CD-sterilized medium induced potato microtuber formation at a similar rate as autoclaved medium. In summary, using CD to sterilize potato medium and vessels did not compromise the growth of seedlings and microtuber induction. This study provides an economical and simplified sterilization method for media used to culture potato plantlets, and this can improve energy use of the large-scale tissue culture industry.

show abstract

Section: Discussionsupporting

confidence: 83%

Use of chlorine dioxide to sterilize medium for tissue culture of potato

Duan

Zhang

Sun

et al. 2019

Sci Rep

View full text Add to dashboard Cite

show abstract

“…ese morphological traits have extensively been used for the screening of germplasm [44][45][46][47]. But the present research has proved that these morphological traits are less 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 Proline 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 effective than the biochemical traits for the rice germplasm screening against drought stress.…”

Section: Discussionmentioning

confidence: 99%

Proline, Total Antioxidant Capacity, and OsP5CS Gene Activity in Radical and Plumule of Rice are Efficient Drought Tolerance Indicator Traits

Saddique

Ali

Sher

et al. 2020

International Journal of Agronomy

View full text Add to dashboard Cite

The success of a plant breeding program is linked with the rapid screening of crop germplasm. In the following study, the germination stage of rice seeds has been examined for the rapid screening of drought-tolerant genotypes. The rice genotypes (10 drought tolerant, 5 moderately drought tolerant, and 5 drought susceptible) were sown in Petri dishes under control and osmotic stress of 15% PEG6000. Data were recorded after four days of sowing for the osmotic stress-induced change in imbibition rate, speed of germination, radical and plumule length, radical and plumule total fresh and dry weight, proline contents, total antioxidant capacity, and malondialdehyde level in radical and plumule of seeds. Moreover, the change in expression of OsP5CS gene was also recorded in one of each drought tolerant, moderately drought tolerant, and drought susceptible genotypes. Under osmotic stress, the level of proline, total antioxidant capacity, and the expression of OsP5CS were increased in drought-tolerant genotypes as compared to moderately drought tolerant and drought susceptible genotypes. While, the change in imbibition rate, speed of germination, radical and plumule length, and fresh and dry weight were not symmetrical in drought tolerant, moderately drought tolerant, and drought susceptible genotypes. In short, the symmetrical change in proline, total antioxidant capacity, and expression of OsP5CS gene within radical and plumule of drought tolerant, moderately drought tolerant, and drought susceptible genotypes can help rapid screening of drought-tolerant rice genotypes.

show abstract

“…The authors found that, on average, water stress reduced seed and forage yield by 38 and 14%, respectively, suggesting a higher impact of drought on reproductive growth than vegetative growth of smooth bromegrass genotypes. In a 2-year field drought study with 36 smooth bromegrass genotypes selected from 25 half-sib polycross progenies, drought overall reduced dry matter yield of smooth brome genotypes by 36 and 39%, relative to non-drought control in 2013 and 2014 (Saeidnia et al, 2017b). Reduction of dry matter yield in bromegrass under drought stress can be explained by reduction in photosynthetic capacity as evidenced by drought-caused reduction in photosynthetic pigments and relative water content.…”

Section: Effects Of Drought On Forage Yield and Qualitymentioning

confidence: 96%

“…These mechanisms are likely to be important under drought and/or heat stress as insufficient water or impaired metabolism impedes full utilization of light energy leading to higher oxidative damage to photosynthetic machinery (Mittler and Zilinskas, 1994;Niyogi, 1999). Photoprotective carotenoids and wax are therefore correlated with drought tolerance of crop plants, including bromegrass (Saeidnia et al, 2017b), and these traits can be measured using canopy/leaf reflectance data derived from high-throughput hyperspectral imaging (Penuelas and Filella, 1998). Higher photoprotective capacity generally contributes to increased quantum efficiency of photosystem II, which can be measured using highthroughput imaging chlorophyll fluorescence.…”

Section: Phenotyping For Abiotic Stress Tolerance and Resource Use-efmentioning

confidence: 99%

Advancing Bromegrass Breeding Through Imaging Phenotyping and Genomic Selection: A Review

et al. 2020

View full text Add to dashboard Cite

Breeding forage crops for high yields of digestible biomass along with improved resourceuse efficiency and wide adaptation is essential to meet future challenges in forage production imposed by growing demand, declining resources, and changing climate. Bromegrasses (Bromus spp.) are economically important forage species in the temperate regions of world, but genetic gain in forage yield of bromegrass is relatively low. In particular, limited breeding efforts have been made in improving abiotic stress tolerance and resource-use efficiency. We conducted a literature review on bromegrass breeding achievements and challenges, global climate change impacts on bromegrass species, and explored the feasibility of applying high-throughput imaging phenotyping techniques and genomic selection for further advances in forage yield and quality selection. Overall genetic gain in forage yield of bromegrass has been low, but genetic improvement in forage yield of smooth bromegrass (Bromus inermis Leyss) is somewhat higher than that of meadow bromegrass (Bromus riparius Rehm). This low genetic gain in bromegrass yield is due to a few factors such as its genetic complexity, lack of long-term breeding effort, and inadequate plant adaptation to changing climate. Studies examining the impacts of global climate change on bromegrass species show that global warming, heat stress, and drought have negative effects on forage yield. A number of useful physiological traits have been identified for genetic improvement to minimize yield loss. Available reports suggest that high-throughput imaging phenotyping techniques, including visual and infrared thermal imaging, imaging hyperspectral spectroscopy, and imaging chlorophyll fluorescence, are capable of capturing images of morphological, physiological, and biochemical traits related to plant growth, yield, and adaptation to abiotic stresses at different scales of organization. The more complex traits such as photosynthetic radiation-use efficiency, water-use efficiency, and nitrogen-use efficiency can be effectively assessed by utilizing combinations of imaging hyperspectral spectroscopy, infrared thermal imaging, and imaging chlorophyll fluorescence techniques in a breeding program. Genomic selection has been applied in the breeding of forage species and the applications show its potential in high ploidy, outcrossing species like bromegrass to improve the accuracy of parental selection and improve

show abstract

Physiological and Tolerance Indices Useful for Drought Tolerance Selection in Smooth Bromegrass

Cited by 17 publications

References 40 publications

Use of chlorine dioxide to sterilize medium for tissue culture of potato

Use of chlorine dioxide to sterilize medium for tissue culture of potato

Proline, Total Antioxidant Capacity, and OsP5CS Gene Activity in Radical and Plumule of Rice are Efficient Drought Tolerance Indicator Traits

Advancing Bromegrass Breeding Through Imaging Phenotyping and Genomic Selection: A Review

Contact Info

Product

Resources

About