Use of chlorine dioxide to sterilize medium for tissue culture of potato

Duan, Yongbo; Zhang, Han; Sun, Meixiu; Zhao, Fenglan; Xue, Tao; Xue, Jing

doi:10.1038/s41598-019-46795-4

Cited by 18 publications

(7 citation statements)

References 30 publications

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“…found that hydrogen dioxide had a better sterilization effect on potato micropropagation and caused no damage to seedling growth and tuber induction [27]. These results indicate that different bacteriostatic agents are suitable for a range of explant sterilization methods.…”

Section: Discussionmentioning

confidence: 81%

Acacia Mangium × A. Auriculiformis Micropropagation in a Non-Sterile Environment

LieJian

Hong

et al. 2021

Preprint

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Background: Autoclaving is used to eliminate contamination during tissue culturing, however, it is a complicated process, time-consuming and costly. Chemical sterilization of tissue culture can effectively eliminate contamination, is a simple procedure, and cost effective. However, studies on the chemical sterilization mostly focus on bud induction, while the effects of chemical sterilization overall process of tissue culture, including bud induction, proliferation, and rooting, remain to be determined. Here, we investigate the effect of chemical sterilization on bud induction, proliferation, and rooting of Acacia mangium × A. auriculiformis.Results: The results showed that chlorothalonil (0.2 g/L) was a suitable chemical sterilant, and bud induction medium was 1/8 Murashige and Skoog medium + agar 7 g/L + chlorothalonil 0.2 g/L + 6-benzylaminopurine 0.5 mg/L The highest induction rate (99.54%) was observed in the third to fifth buds’ stem segments collected in October treated with 0.8 g/L carbendazim for 3 min, with a contamination rate of 0. The rooting medium was agar 7 g/L + chlorothalonil 0.2 g/L+ indolebutyric acid 1.5 mg/L + naphthylacetic acid 0.5 mg/L, and the rooting rate was 97.62%. The proliferation rate and subculture duration showed a positive correlation, while the proliferation rate was 3.58 times higher at the fourth subculture rooting. Conclusions: Our results suggest that chlorothalonil can effectively replace autoclaving during bud induction, proliferation, and rooting of A. mangium × A. auriculiformis. The findings of this study provide technical support for rapid seedlings propagation, accelerates the breeding process of Acacia, and can be applied in other tree species.

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Section: Discussionmentioning

confidence: 81%

Acacia Mangium × A. Auriculiformis Micropropagation in a Non-Sterile Environment

LieJian

Hong

et al. 2021

Preprint

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“…Chlorine dioxide was also used to sterilize the medium for tissue culture of potato ( Solanum tuberosum L .) [ 56 ]. NaOCl can alleviate significantly the inhibitive effect of salt stress during the germination phase.…”

Section: Resultsmentioning

confidence: 99%

Establishment of optimized in vitro disinfection protocol of Pistacia vera L. explants mediated a computational approach: multilayer perceptron–multi−objective genetic algorithm

2022

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Background Contamination−free culture is a prerequisite for the success of in vitro − based plant biotechnology. Aseptic initiation is an extremely strenuous stride, particularly in woody species. Meanwhile, over−sterilization is potentially detrimental to plant tissue. The recent rise of machine learning algorithms in plant tissue culture proposes an advanced interpretive tool for the combinational effect of influential factors for such in vitro − based steps. Results A multilayer perceptron (MLP) model of artificial neural network (ANN) was implemented with four inputs, three sterilizing chemicals at various concentrations and the immersion time, and two outputs, disinfection efficiency (DE) and negative disinfection effect (NDE), intending to assess twenty−seven disinfection procedures of Pistacia vera L. seeds. Mercury chloride (HgCl2; 0.05–0.2%; 5–15 min) appears the most effective with 100% DE, then hydrogen peroxide (H2O2; 5.25–12.25%; 10–30 min) with 66–100% DE, followed by 27–77% DE for sodium hypochlorite (NaOCl; 0.54–1.26% w/v; 10–30 min). Concurrently, NDE was detected, including chlorosis, hard embryo germination, embryo deformation, and browning tissue, namely, a low repercussion with NaOCl (0–14%), a moderate impact with H2O2 (6–46%), and pronounced damage with HgCl2 (22–100%). Developed ANN showed R values of 0.9658, 0.9653, 0.8937, and 0.9454 for training, validation, testing, and all sets, respectively, which revealed the uprightness of the model. Subsequently, the model was linked to multi−objective genetic algorithm (MOGA) which proposed an optimized combination of 0.56% NaOCl, 12.23% H2O2, and 0.068% HgCl2 for 5.022 min. The validation assay reflects the high utility and accuracy of the model with maximum DE (100%) and lower phytotoxicity (7.1%). Conclusion In one more case, machine learning algorithms emphasized their ability to resolve commonly encountered problems. The current successful implementation of MLP–MOGA inspires its application for more complicated plant tissue culture processes.

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“…Using chemicals instead of autoclaving has drawn attention because it is an easily applicable method to decontaminate culture medium. Several chemicals, including antibiotics [13], fungicides [14], plant preservative mixtures [15], chlorine dioxide [16,17], diethyl pyrocarbonate [18], peracetic acid [19], sodium hypochlorite [20][21][22], hydrogen peroxide [23],…”

Section: Introductionmentioning

confidence: 99%

Effect of biosynthesised silver nanoparticles as sterilant on physiological and biochemical characteristics in micropropagation of Musa sapientum L.

Phongtongpasuk,

Liamnimit,

Buakaew

et al. 2024

Adv. Nat. Sci: Nanosci. Nanotechnol.

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Green synthesis of silver nanoparticles (AgNPs) using fruit peel extract has gained considerable interest, as it is an eco-friendly and cost-effective method. However, studies on the use of biosynthesised AgNPs to sterilise plant tissue culture medium as an alternative to autoclaving are limited. Thus, this study presented a biogenic method for synthesising AgNPs using mangosteen peel extract. The biosynthesised AgNPs were characterised by different spectroscopic and microscopic methods, including UV-visible spectroscopy (UV-vis), Fourier transform infrared spectroscopy, X-ray diffraction, transmission electron microscopy, and energy dispersive x-ray spectroscopy. The results showed that the phytochemical constituents in the mangosteen peel extract (MPE) helped to reduce Ag+ from AgNO3 to metallic silver (Ag0) and stabilise the particles. The formation of biogenic AgNPs was monitored by UV-vis demonstrating a characteristic peak at 425 nm. The AgNPs were spherical and crystalline. The size distribution of the biogenic AgNPs was 5–47 nm with an average diameter of 23.1 ± 6.8 nm. To evaluate the potential of the AgNPs for use as an alternative method to sterilise the culture medium, the antimicrobial activity of the biosynthesised AgNPs (0.1, 1, 10, 100, and 1000 mg l−1) was tested in the banana culture medium. The most feasible concentration of AgNPs to decontaminate the culture medium was 100 mg l−1. Moreover, the results demonstrated that adding 100 mg l−1 AgNPs to the culture medium promoted the growth of the plantlets without any toxic effects. Thus, biogenic AgNPs are a potential biocide to sterilise in vitro banana culture medium.

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Use of chlorine dioxide to sterilize medium for tissue culture of potato

Cited by 18 publications

References 30 publications

Acacia Mangium × A. Auriculiformis Micropropagation in a Non-Sterile Environment

Acacia Mangium × A. Auriculiformis Micropropagation in a Non-Sterile Environment

Establishment of optimized in vitro disinfection protocol of Pistacia vera L. explants mediated a computational approach: multilayer perceptron–multi−objective genetic algorithm

Effect of biosynthesised silver nanoparticles as sterilant on physiological and biochemical characteristics in micropropagation of Musa sapientum L.

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