Physiological and Biochemical Characterization of a Novel Nicotine-Degrading Bacterium Pseudomonas geniculata N1

Liu, Yanghui; Wang, Limin; Huang, Kaiming; Wang, Weiwei; Nie, Xueling; Jiang, Yi; Li, Pengpeng; Liu, Shanshan; Xu, Ping; Tang, Hongzhi

doi:10.1371/journal.pone.0084399

Cited by 20 publications

(14 citation statements)

References 25 publications

(26 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…P. geniculata has much more potential applications in the field of environment protection. As a strain of environmental origin, P. geniculata has been reported capable of degrading phenanthrene, T-2 toxin, nicotine, and industrial dyes ( Shi et al, 2013 ; Yang et al, 2013 ; Liu et al, 2014 ), and also active in nitrogen fixation, plant-growth promotion, and seedling growth of aged seeds ( Zhang et al, 2010 ; Gopalakrishnan et al, 2015 ; Liu et al, 2019 ). In this study, we identify a new anaerobic metabolism of P. geniculata PQ01 that can effectively reduce soluble humics analogs and insoluble Fe(III) (hydr)oxides under anaerobic conditions, using glucose, sucrose, acetic acid, formic acid, or pyruvic acid as the electron donor.…”

Section: Discussionmentioning

confidence: 99%

A Newly Discovered Humic-Reducing Bacterium, Pseudomonas geniculata PQ01, Isolated From Paddy Soil Promotes Paraquat Anaerobic Transformation

Chen

et al. 2020

Front. Microbiol.

View full text Add to dashboard Cite

Due to toxicity and persistence of paraquat (a widely used herbicide), eco-friendly remediation approaches to its contamination and effective antidotes to its poisoning have been highly desired and raised increasing concerns. Paraquat degradation was lesser in aerobic soil in comparison with anaerobic soil, and humic-reducing microorganisms (HRMs) play a key role in paraquat anaerobic transformation process. However, the degradation pathways and related mechanisms remain poorly understood. In this study, we investigated the specific interaction mechanisms of the paraquat transformation processes mediated by a humic-reducing strain under anaerobic conditions. A strain of pure culture, designated as PQ01, was successfully isolated from paddy soil using anaerobic enrichment procedure, and identified as Pseudomonas geniculata using phenotypic and phylogenetic analysis. Sucrose, glucose, pyruvate, formic acid, and acetic acid were shown to be favorable electron donors for the reduction of anthrahydroquinone-2,6-disulfonate (AQDS) reduction by PQ01. The strain also had the ability of reducing Fe(III) (hydr)oxides in the presence of sucrose with efficiencies in the order of ferrihydrite > α-FeOOH/γ-FeOOH > γ-Fe 2 O 3 > α-Fe 2 O 3 . In the “PQ01 + paraquat + AQDS + sucrose” system, AQDS reduction and paraquat biotransformation by strain PQ01 occurred simultaneously, and the presence of sucrose significantly enhanced the biotransformation. Specific mechanisms of the electron transfer processes are promoted by both PQ01 and AQDS, and proceed in two aspects: (1) paraquat served as electron donor in the anaerobic reduction of AQDS by strain PQ01; (2) AQDS was reduced by PQ01 anaerobic metabolism to produce AH 2 QDS, which can directly react with paraquat under anaerobic conditions to generate a single crystal compound (molecular formula of the unit structure is C 2 6 H 2 0 N 2 O 8 S 2 ), causing the paraquat to decline dramatically. In conclusion, this main mechanism included the microbial reduction of AQDS to AH 2 QDS, followed by the abiotic reaction between AH 2 QDS and paraquat. This study reported the new characteristics of P. geniculata capable of reducing humics analogs, Fe(III) (hydr)oxides, and paraquat, and proposed a novel electron transformation mechanism of the HRMs’ mediated degradation of organic contaminants.

show abstract

Section: Discussionmentioning

confidence: 99%

A Newly Discovered Humic-Reducing Bacterium, Pseudomonas geniculata PQ01, Isolated From Paddy Soil Promotes Paraquat Anaerobic Transformation

Chen

et al. 2020

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…The standard strain (P. agglomerans, CGMCC 1.843), collected from China General Microbiological Culture Collection Center (CGMCC), was used as a control. The biochemical properties including Gram's stain, starch hydrolysis, gelatin liquefaction, nitrate reduction, catalase reaction, MR reaction, VP reaction, indole production, levan formation, potato decay, H 2 S production, urease reaction, oxidase reaction, motility, and esculin hydrolysis, were tested using standard methods (Liu et al, 2014;Yazdani et al, 2018). The isolated strain was identified by referring to the results of the standard strain and Bergey's Manual of Determinative Bacteriology (Holt, 1994).…”

Section: Physiological and Biochemical Characterizationmentioning

confidence: 99%

A new bacterial leaf blight disease of oat (Avena sativa) caused by Pantoea agglomerans in China

et al. 2021

View full text Add to dashboard Cite

A new leaf blight disease of oat (Avena sativa) was observed in many oat fields in Huan county, Gansu Province of China, during 2018–2019. Typical symptoms appeared as yellow necrotic and water‐soaked lesions. The lesions developed from tip to base of leaves and eventually resulted in leaf withering. Disease incidence on plants (leaves) was approximately 36%–100%. A gram‐negative bacterium was isolated from the necrotic lesions of all samples. Coupled with multigene sequence (16S rRNA and gyrB gene) analyses, Biolog Gen III MicroStation, morphological, physiological, and biochemical characterization identified the pathogen as Pantoea agglomerans. Pathogenicity tests by wounding and injection inoculations in the greenhouse established that P. agglomerans could induce typical symptoms as observed in the field. The infection rate in leaves was 44%–71% after 14 days. In addition, host range tests showed that P. agglomerans could infect other plant hosts, including Sorghum sudanense, Medicago sativa, Fagopyrum esculentum, Setaria italica, and Zea mays. This is the first report of P. agglomerans causing bacterial leaf blight disease (LBD) on oat in China. The current study can provide a foundation for the prevention of this disease in the future.

show abstract

“…ZJUSKD [ 24 ], Pseudomonas putida J5 [ 25 ], Pseudomonas putida HF-1 [ 26 ], Pseudomonas plecoglossicida TND35 [ 27 ], Pseudomonas sp. HZN6 [ 28 , 29 , 30 ], Pseudomonas geniculata N1 [ 31 ], Shinella sp. HZN7 [ 16 ], Agrobacterium tumefaciens S33 [ 32 ], Ochrobactrum sp.…”

Section: Figurementioning

confidence: 99%

Characterization and Genome Analysis of a Nicotine and Nicotinic Acid-Degrading Strain Pseudomonas putida JQ581 Isolated from Marine

Qiu

Chen

et al. 2017

Marine Drugs

View full text Add to dashboard Cite

Abstract:The presence of nicotine and nicotinic acid (NA) in the marine environment has caused great harm to human health and the natural environment. Therefore, there is an urgent need to use efficient and economical methods to remove such pollutants from the environment. In this study, a nicotine and NA-degrading bacterium-strain JQ581-was isolated from sediment from the East China Sea and identified as a member of Pseudomonas putida based on morphology, physio-biochemical characteristics, and 16S rDNA gene analysis. The relationship between growth and nicotine/NA degradation suggested that strain JQ581 was a good candidate for applications in the bioaugmentation treatment of nicotine/NA contamination. The degradation intermediates of nicotine are pseudooxynicotine (PN) and 3-succinoyl-pyridine (SP) based on UV, high performance liquid chromatography, and liquid chromatography-mass spectrometry analyses. However, 6-hydroxy-3-succinoyl-pyridine (HSP) was not detected. NA degradation intermediates were identified as 6-hydroxynicotinic acid (6HNA). The whole genome of strain JQ581 was sequenced and analyzed. Genome sequence analysis revealed that strain JQ581 contained the gene clusters for nicotine and NA degradation. This is the first report where a marine-derived Pseudomonas strain had the ability to degrade nicotine and NA simultaneously.

show abstract

Physiological and Biochemical Characterization of a Novel Nicotine-Degrading Bacterium Pseudomonas geniculata N1

Cited by 20 publications

References 25 publications

A Newly Discovered Humic-Reducing Bacterium, Pseudomonas geniculata PQ01, Isolated From Paddy Soil Promotes Paraquat Anaerobic Transformation

A Newly Discovered Humic-Reducing Bacterium, Pseudomonas geniculata PQ01, Isolated From Paddy Soil Promotes Paraquat Anaerobic Transformation

A new bacterial leaf blight disease of oat (Avena sativa) caused by Pantoea agglomerans in China

Characterization and Genome Analysis of a Nicotine and Nicotinic Acid-Degrading Strain Pseudomonas putida JQ581 Isolated from Marine

Contact Info

Product

Resources

About