A new bacterial leaf blight disease of oat (Avena sativa) caused by Pantoea agglomerans in China

Abstract

A new leaf blight disease of oat (Avena sativa) was observed in many oat fields in Huan county, Gansu Province of China, during 2018–2019. Typical symptoms appeared as yellow necrotic and water‐soaked lesions. The lesions developed from tip to base of leaves and eventually resulted in leaf withering. Disease incidence on plants (leaves) was approximately 36%–100%. A gram‐negative bacterium was isolated from the necrotic lesions of all samples. Coupled with multigene sequence (16S rRNA and gyrB gene) analyses, Biolog Gen III MicroStation, morphological, physiological, and biochemical characterization identified the pathogen as Pantoea agglomerans. Pathogenicity tests by wounding and injection inoculations in the greenhouse established that P. agglomerans could induce typical symptoms as observed in the field. The infection rate in leaves was 44%–71% after 14 days. In addition, host range tests showed that P. agglomerans could infect other plant hosts, including Sorghum sudanense, Medicago sativa, Fagopyrum esculentum, Setaria italica, and Zea mays. This is the first report of P. agglomerans causing bacterial leaf blight disease (LBD) on oat in China. The current study can provide a foundation for the prevention of this disease in the future.

Evaluation of different antimicrobial agents for laboratory and field against Pantoea agglomerans, the causative agent of bacterial leaf blight disease on oat (Avena sativa)

“…Isolation and identification of P. agglomerans causing LBD was reported in our previous study (Wang, Chen, Xue, et al, 2022). P .…”

“…Isolation and identification of P. agglomerans causing LBD was reported in our previous study (Wang, Chen, Xue, et al, 2022). P. agglomerans strains were grown on nutrient agar (NA) in dark conditions for 24-48 h at 25°C and suspended in sterile distilled water to obtain approximately 10 8 cfu/mL based on optical density readings using a turbidimeter (Biolog).…”

“…agglomerans was frequently observed in oat‐growing areas of Huan county, Gansu province in north‐western China. This new disease of oat decreased seed germination, seedling growth and seed quality (Wang, Chen, White, et al, 2022; Wang, Chen, Xue, et al, 2022).…”

Genomics assisted functional characterization of Bacillus velezensis E as a biocontrol and growth promoting bacterium for lily

“…However, there is no report of the antagonistic effect of B. velezensis on Fusarium equiseti , Xanthomonas arboricola , and Pantoea agglomerans . Among them, Pantoea agglomerans can cause bacterial shot-hole disease of plum, bacterial soft rot of Dioscorea opposita , blight disease on Solanum muricatum and oat, necrotic disease on Ziziphus jujuba , brown apical necrosis of walnut, and bacterial shot-hole disease of plum ( Yang et al, 2011 ; She et al, 2019 ; Moloto et al, 2020 ; She et al, 2021 ; Fan et al, 2022 ; Wang et al, 2022 ). Xanthomonas arboricola can cause bacterial canker and spot and bacterial blight diseases of stone fruit, almond, and walnut trees, respectively ( Lamichhane, 2014 ).…”

Comparative study on antimicrobial activity of mono-rhamnolipid and di-rhamnolipid and exploration of cost-effective antimicrobial agents for agricultural applications

“…alternata, P. agglomerans, Cladosporium sp., P. oxalicum were potential plant pathogens in agriculture[24][25][26]. Mono-rhamnolipid and di-rhamnolipid exhibited antimicrobial activity to the potential agricultural pathogens such as A. alternata, P. agglomerans and Cladosporium sp.…”