Optically pure D-lactate production has received much attention for its critical role in high-performance polylactic acid production. However, the current technology can hardly meet the comprehensive demand of industrialization on final titer, productivity, optical purity, and raw material costs. Here, an efficient D-lactate producer strain, Sporolactobacillus terrae (S. terrae) HKM-1, is isolated for D-lactate production. The strain HKM-1 shows extremely high D-lactate fermentative capability by using peanut meal, soybean meal, or corn steep liquor powder as a sole nitrogen source; the final titers (205.7 g L −1 , 218.9 g L −1 , and 193.9 g L −1 , respectively) and productivities (5.56 g L −1 h −1 , 5.34 g L −1 h −1 , and 3.73 g L −1 h −1 , respectively) of D-lactate reached the highest level ever reported. A comparative genomic analysis between S. terrae HKM-1 and previously reported D-lactate high-producing Sporolactobacillus inulinus (S. inulinus) CASD is conducted. The results show that many unrelated genetic features may contribute to the superior performance in D-lactate production of S. terrae HKM-1. This D-lactate producer HKM-1, along with its fermentation process, is promising for sustainable D-lactate production by using agroindustrial wastes.
Management of solid wastes with high nicotine content, such as those accumulated during tobacco manufacturing, poses a major challenge, which can be addressed by using bacteria such as Pseudomonas and Arthrobacter. In this study, a new species of Pseudomonas geniculata, namely strain N1, which is capable of efficiently degrading nicotine, was isolated and identified. The optimal growth conditions for strain N1 are a temperature of 30°C, and a pH 6.5, at a rotation rate of 120 rpm min−1 with 1 g l−1 nicotine as the sole source of carbon and nitrogen. Myosmine, cotinine, 6-hydroxynicotine, 6-hydroxy-N-methylmyosmine, and 6-hydroxy-pseudooxynicotine were detected as the five intermediates through gas chromatography-mass and liquid chromatography-mass analyses. The identified metabolites were different from those generated by Pseudomonas putida strains. The analysis also highlighted the bacterial metabolic diversity in relation to nicotine degradation by different Pseudomonas strains.
A newly isolated bacterium, Pseudomonas geniculata N1, can efficiently degrade nicotine. Here we present a 4.51-Mb assembly of its genome, which is the first sequence of the P. geniculata group. The sequence contains the genes related to nicotine catabolism and may provide insights into its molecular mechanism for N-heterocyclic degradation.
Sporolactobacillus terrae DSM 11697 is the type strain of S. terrae. Here, we present a 3.2-Mb assembly of its genome sequence. As S. terrae is one of the important lactic acid bacteria, the genome sequence may provide insights into the molecular mechanism for its further microbial investigation.
The PLA‐derived materials possess great potential of commercialization. A soil‐derived bacterium was isolated for effi cient production of optically pure d‐lactate, a crucial monomer for high‐performance PLA. This will be a promotion for replacing petroleum‐based plastics with bio‐based polymers which can be easily mineralized by soil microbes. This is reported by Xiao Han, Kaiming Huang, Hongzhi Tang, Jun Ni, Jiongqin Liu, Ping Xu, and Fei Tao in the article https://doi.org/10.1002/biot.201800656.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.