Physiochemical and biological characterization of novel exopolysaccharide produced by Bacillus tequilensis FR9 isolated from chicken

Rani, Rizwana Parveen; Anandharaj, Marimuthu; Sabhapathy, Palani; Ravindran, A. David

doi:10.1016/j.ijbiomac.2016.11.122

Cited by 82 publications

(43 citation statements)

References 36 publications

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“…The weak stretch band at 802.64 cm -1 was the main character for α-D-glucose [27]. The peak in the region of 668.71 cm -1 and 551.20 cm -1 indicated the existence of glycosidic linkage bonds between the glycosyl groups [26].…”

Section: A C C E P T E Dmentioning

confidence: 94%

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Characterization and Antioxidant Activity of the Exopolysaccharide Produced by Bacillus amyloliquefaciens GSBa-1

Zhao¹,

Zhang²,

Jiang³

et al. 2018

Journal of Microbiology and Biotechnology

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The exopolysaccharide (EPS) produced by Bacillus amyloliquefaciens GSBa-1 was isolated and purified by ethanol precipitation, DEAE-cellulose and Sepharose CL-6B chromatography. The molecular weight of the purified EPS was determined to be 54 kDa. Monosaccharide analysis showed that the EPS was composed of predominantly glucose, and it was further confirmed by NMR spectroscopy to be α-glucan consisted of a trisaccharide repeating unit with possible presence of two α-(1→3) and one α-(1→6) glucosidic linkages. Microstructural analysis showed that the EPS appeared as ellipsoid or globose with smooth surface. The EPS had a degration temperature at 240℃. Furthermore, the EPS had strong DPPH and hydroxyl radical scavenging activities, and moderate scavenging activities on superoxidant anion and metal ion chelating activity. This is the first characterization of a glucan produced by B. amyloliquefaciens with strong antioxidant activity. The results of this study suggested the potential of the EPS from B. amyloliquefaciens GSBa-1 to serve as a natural antioxidant for application in functional products.

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Section: A C C E P T E Dmentioning

confidence: 94%

“…licheniformis produced an EPS composed of glucose, galactose and mannose, while B. tequilensis FR9 produced an EPS composed of glucose, galactose, mannose, arabinose and xylose[26]. B. amyloliquefaciens LPL061 produced two different EPSs composed of mannose and glucose in a relative molar proportion of 96.9/3.2 and 65.3/34.7, respectively[13].…”

mentioning

confidence: 99%

Characterization and Antioxidant Activity of the Exopolysaccharide Produced by Bacillus amyloliquefaciens GSBa-1

Zhao¹,

Zhang²,

Jiang³

et al. 2018

Journal of Microbiology and Biotechnology

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“…A parallel increase between the EPS concentrations and the biomass was observed during the first 6 h of fermentation, reaching a maximum EPS yield of 15.61 g/L, then declined between 6 h and 12 h, and remained constant afterwards (Figure 6b). The time to obtain the maximum EPS was much shorter than those of B. tequilensis PS21 in a previous study [35], and the EPS yield of CGMCC 17603 (15.61 g/L) was more than that of B. tequilensis PS21, B. tequilensis FR9 (6.83 g/L), and B. sonorensis MJM60315 (8.4 g/L) [35,44,45], indicating that the production mode of CGMCC 17603 can greatly shorten the production time and increase the production efficiency in commercial production. Therefore, we have realized high-density culture of B. tequilensis CGMCC 17603 and improvement of EPS yield through low-cost, short-time, and simple production processes, and provided new solutions for the development of strain preparations and EPS preparations.…”

Section: Sand-fixing Property Of B Tequilensis Cgmcc 17603mentioning

confidence: 69%

A New Strain of Bacillus tequilensis CGMCC 17603 Isolated from Biological Soil Crusts: A Promising Sand-Fixation Agent for Desertification Control

Zhao

Wang

et al. 2019

Sustainability

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In arid and semi-arid desert ecosystems, physical, chemical, and vegetative measures were used to prevent wind erosion. However, studies on the utilization of microbial resources for sand fixation are still limited. To fill this gap, a new strain of Bacillus tequilensis CGMCC 17603 with high productivity of exopolysaccharide (EPS) was isolated from biological soil crusts, and its high-density culture technology and sand-fixing ability were studied. The one-factor-at-a-time approach (OFAT) and Box–Behnken design of CGMCC 17603 showed that the optimum culture conditions were pH 8.5, temperature 31 °C, agitation speed 230 rpm, and inoculation quantity 3%, and the optimum medium was 27.25 g/L glucose, 15.90 g/L yeast extract, and 5.61 g/L MgSO4•7H2O. High-density culture showed that the biomass and EPS yield of CGMCC 17603 increased from 9.62 × 107 to 2.33 × 109 CFU/mL, and from 8.01 to 15.61 g/L, respectively. The field experiments showed that CGMCC 17603 could effectively improve the ability of sand fixation and wind prevention. These results indicated that B. tequilensis, first isolated from cyanobacterial crusts, can be considered as an ideal soil-fixing agent to combat desertification in arid and semi-arid areas.

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“…While compact structure of EPS indicated its potential to be used as viscosifier, thickener or stabilizing agent for use in food industry because of plasticized film like property (Sajna et al 2013); porous EPS structure were proven to be a good candidate for heavy metal biosorption (Qiang et al 2013), while also having the capillary force to hold more water (Rani et al 2017). The fibrous pores of bacterial EPSs were already proved to help bacterial cells to stay attached to each other due to high affinity interactions (Rani et al 2017). Thus the psychrohalophilic bacterial isolates from SO explored in this study may have produced EPSs as an extracellular response to the environmental constraints.…”

Section: Discussionmentioning

confidence: 99%

Bacterial exopolysaccharides from extreme marine habitat of Southern Ocean: Production and partial characterization

Banerjee

Gupta²,

Nigam³

et al. 2019

Gayana (Concepc.)

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Deep marine microorganisms survive under extreme ecological settings and harsh environmental conditions of low temperature, high salinity, and high atmospheric pressure making it significant of scientific interest. Southern Ocean (SO) is one such example of deep marine ecosystem and the microorganisms inhabiting in such hostile environment may produce different bioactive secondary metabolites. SO (Indian Sector) is relatively less documented in terms of microbial composition and community dynamics. The present study involves isolation of exopolysaccharides (EPSs) from three potent SO (Indian Sector) bacteria, optimization of the EPS production and partial characterization of them. Three different EPSs show varying structural conformation, that is from porous to strong flakes mimicking polymeric structure with C/N ratio ranging between 4-11. FTIR spectra have exhibited the presence of different active groups of carbohydrate moieties, water molecules and protein-associated amides. EPSs produced by marine microorganisms show high biotechnological promises such as drug carrier in pharmaceutical field, emulsifier and cryoprotectant in food-processing industry, detoxification of petrochemical oils and much more. The three bacterial isolates in this study showed potential of producing EPS biopolymer that can be further explored in terms of its proper biotechnological applications.

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Physiochemical and biological characterization of novel exopolysaccharide produced by Bacillus tequilensis FR9 isolated from chicken

Cited by 82 publications

References 36 publications

Characterization and Antioxidant Activity of the Exopolysaccharide Produced by Bacillus amyloliquefaciens GSBa-1

Characterization and Antioxidant Activity of the Exopolysaccharide Produced by Bacillus amyloliquefaciens GSBa-1

A New Strain of Bacillus tequilensis CGMCC 17603 Isolated from Biological Soil Crusts: A Promising Sand-Fixation Agent for Desertification Control

Bacterial exopolysaccharides from extreme marine habitat of Southern Ocean: Production and partial characterization

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