Physicochemical properties and antioxidant capacity of propolis of stingless bees (Meliponinae) and Apisfrom two regions of Tocantins, Brazil

Abstract

The composition of propolis depends on time, vegetation and the location of the collection area. The objective of this study was to determine the physicochemical characteristics, the concentration of phenol compounds and the antioxidant capacity of propolis of native stingless bees (Meliponinae) and Apis from the State of Tocantins. Extraction with 80% ethanol (v/v) was performed in order to obtain the extracts. Parameters examined were: propolis mass loss by desiccation at 105 ºC, ashes, wax concentration and pH. Furthermore, the propolis antioxidant activity was measured, as well as the total concentration of phenol compounds. The extracts were also analyzed by high performance liquid chromatography. The total concentration of phenol compounds varied between 121.78 and 631.29 (mg GAE g . High performance liquid chromatography analysis allowed us to infer the existence of phenol compounds. The results indicated that the studied propolis samples constitute good sources of natural antioxidants. The variety of phenol compounds identified in this study, and the diverse biological functions reported in literature for these compounds indicated that this stingless bee propolis (Meliponinae) and Apis has a high pharmacological potential. KEYWORDS: Propolis, antioxidant, phenol compounds.Propriedades físico-químicas e capacidade antioxidante de própolis de abelhas sem ferrão (Meliponinae) e Apis de duas regiões do Tocantins, Brasil RESUMO A composição da própolis depende do tempo, da vegetação e a localização da área de coleta. Este estudo teve como objetivo determinar as características físicas e químicas, o teor de compostos fenólicos e capacidade antioxidante da própolis de abelhas nativas sem ferrão (Meliponinae) e Apis do Estado do Tocantins. Para a obtenção dos extratos empregou-se extração com etanol 80% (v/v). Os parâmetros analisados foram: perda de peso por dessecação a 105 °C, cinzas, Teor de cera e pH. Além dessas foi mensurada a atividade antioxidante e o teor de compostos fenólicos totais. Adicionalmente os extratos de própolis foram também analisados por cromatografia líquida de alta eficiência. O teor de compostos fenólicos totais variou entre 121,78 e 631,29 (mg GAE g -1 ). A atividade antioxidante expressa pelo valor de CE 50 variou entre 29,81 e 845,38 µg mL -1 . As análises por cromatografia líquida de alta eficiência permitiram inferir a existência de compostos fenólicos. Os resultados indicaram que as amostras de própolis estudadas constituem boas fontes de antioxidantes naturais. A variedade de compostos fenólicos identificada neste estudo, e as diversas funções biológicas relatadas na literatura para estes compostos, indicaram que a própolis de abelhas sem ferrão (Meliponinae) e Apis tem um grande potencial farmacológico. PALAVRAS-CHAVE: Própolis, antioxidante, compostos fenólicos. Physicochemical properties and antioxidant capacity of propolis of stingless bees (Meliponinae) and Apis from two regions of Tocantins, Brazil ACTA

Chemical composition and antioxidant activity of geopropolis produced by Melipona fasciculata (Meliponinae) in flooded fields and cerrado areas of Maranhão State, northeastern Brazil

“…Gallic acid and its derivatives have been identified in geopropolis and propolis of stingless bees in the Brazilian states of Maranhão (Dutra et al 2014), Pernambuco, Paraná, São Paulo (Velikova et al 2000), Piauí (Bankova et al 1998) and Tocantins (Araújo et al 2016).…”

Phytochemical Profile and Antioxidant Activity of Propolis Ethanolic Extract from Tetragonula Bee

“…The use of propolis with antioxidant capacity for prevention and treatment of diseases related to the increase of oxidative stress such as cancer, aging, and cardiovascular diseases. 4 This research aimed to determine the phytochemical composition and the antioxidant properties in vitro of three types propolis collected from the region of South Sulawesi Province of Indonesia. 20 µL of CH 3 COOK(1M) and 140 µL of distilled water, and shaken for 60s.…”

“…3 The composition of propolis depends on diverse vegetation, phytogeographic region, and time of the collection. 4 Results from previous researchers found more than 300 components contained in propolis i.e. aromatic acids; aromatics esters; flavanones; flavones and flavonol; chalcones and dihydrochalcones; terpenoids; acyclic hydrocarbons and esters; alcohols; aliphatic acids (short-chain); aliphatic esters; aliphatic fatty acids (long-chain) and esters; amino acids; aromatic hydrocarbons; acetophenones and other ketones; glycerol derivatives; steroids; sugars and sugar alcohols; and miscellaneous ingredients.…”

Beekeeping in Brazil: A Bibliographic Review

“…were valuable for their characterization. The results obtained provide support to know the possible species suppliers of resin for Tubi, increasing the scientific knowledge about the propolis of stingless bees that are still incipient, and may also contribute to the protection of this species of bee in the elaboration of conservation programs and replanting of their areas of occupation 08 Araújo et al [18] Propolis samples collected in the two regions of Tocantins presented concentrations of moisture, ash, and wax that fall under Brazilian legislation for propolis quality. They also evidence a high concentration of phenol compounds and good antioxidant capacity.…”