Physicochemical properties and antioxidant activities of polysaccharides from Gynura procumbens leaves by fractional precipitation

Li, Jingen; Wang, Wenjun; Zheng, Guodong; Li, Linyan

doi:10.1016/j.ijbiomac.2016.11.113

Cited by 64 publications

(37 citation statements)

References 23 publications

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“…The amount of total sugar and uronic acid of FVYs was higher than the corresponding fractions of FVWs. Protein and uronic acid were detected in FVYs and FVWs, which suggests that all of the samples belong to acid glycoprotein compounds [31].…”

Section: Resultsmentioning

confidence: 99%

Characterization and Antioxidant Activities of Yellow Strain Flammulina velutipes (Jinhua Mushroom) Polysaccharides and Their Effects on ROS Content in L929 Cell

Sung

Chou³

et al. 2019

Antioxidants

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Yellow strain Flammulina velutipes, which is known as Jinhua mushroom in Taiwan, has become popular among customers due to its distinct texture that is utterly different from white strain F. velutipes. However, there has been little study on the physicochemical properties, antioxidant activities, and biological functions of yellow strain F. velutipes polysaccharides (FVYs). The specific aims of this study are to evaluate and compare the physicochemical properties, antioxidant activities, and biological functions of FVYs and white strain F. velutipes polysaccharides (FVWs) in order to select the strain appropriate for cosmetic ingredient. The FVYs and FVWs were prepared by fractional precipitation (40%, 60%, and 80%). According to the results, FVY-80 showed the greatest antioxidant activities based on 2,2-diphenyl-1-picrylhydrazyl (DPPH) (IC50 = 2.22 mg/mL) and 2,2’ -azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radical assay (IC50 = 2.04 mg/mL). None of the fractions exhibited cytotoxicity toward L929 cell under a concentration of 500 μ g/mL. FVY-80 significantly reduced the reactive oxygen species (ROS) content in L929 cell by 55.96%, as compared with the H2O2-induced L929 cell, according to the dichloro-dihydro-fluorescein diacetate (DCFH-DA) assay. In conclusion, we suggest that FVY-80 is the best source for a cosmetics ingredient.

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Section: Resultsmentioning

confidence: 99%

Characterization and Antioxidant Activities of Yellow Strain Flammulina velutipes (Jinhua Mushroom) Polysaccharides and Their Effects on ROS Content in L929 Cell

Sung

Chou³

et al. 2019

Antioxidants

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“…us, an appropriate molecular weight is a critical factor for the biological activity of the polysaccharide. For example, a polysaccharide DOPA-1 from Dendrobium officinale in Huoshan has an average molecular weight of 229 kDa close to the upper limit of our suitable molecular weight range (176.29 kDa), resulting in induction of apoptosis in tumor cells through altered mitochondrial function, ROS production, and altered apoptosis-related protein expression [30]. Similarly, the polysaccharide DCPP1a-1 isolated from the protocorms of Dendrobium officinale with a molecular weight of 189 kDa showed significant antitumor effects on H22-bearing mice and significantly raised the thymus and spleen indexes [31].…”

Section: Discussionmentioning

confidence: 99%

Physicochemical and Antioxidant Properties of the Degradations of Polysaccharides from Dendrobium officinale and Their Suitable Molecular Weight Range on Inducing HeLa Cell Apoptosis

Zhang

Luo

Wei

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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Different molecular weight polysaccharides of Dendrobium officinale (DOPs) have gradually attracted attention because of their broad biological activities. They, however, remain poorly defined whether their antitumor activity is associated with molecular weight. In this study, the physicochemical, antioxidant, and antitumor properties of DOPs, including the crude polysaccharide (DOP) and its six degradation fractions (DOP1–DOP6) extracted from Dendrobium officinale, were determined. Consequently, DOPs were mainly composed of different ratios of mannose and glucose as follows: 5.15 : 1, 4.62 : 1, 4.19 : 1, 4.46 : 1, 4.32 : 1, 4.29 : 1, and 4.23 : 1, and their molecular weights were significantly different ranging from 652.29 kDa to 11.10 kDa. With the concentration increase of DOPs, the scavenging capacity against OH and DPPH free radicals increased. The antitumor ability of DOPs was different that DOP1–DOP5 (Mw: 176.29 kDa–28.48 kDa) exhibited the best antiproliferation activity than DOP (Mw: 652.29 kDa) and DOP6 (Mw: 11.10 kDa) in HeLa cells rather than PC9, A549, and HepG2 cells. Moreover, it is worth mentioning that DOP1 and DOP5 showed stronger capability on inducing apoptosis of HeLa cells than DOP and DOP6 via the mitochondrial pathway by upregulating the ratio of the Bax/Bal-2 mRNA expression. The results demonstrated that DOPs can be used as the potential natural antioxidant and antitumor products in pharmaceutical industries, and the molecular weight is a crucial influential factor of their antitumor activity that 28.48 kDa–176.29 kDa is a suitable range we may refer to.

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“…At 6000 lg/mL, the absorbance of WE, EAE and ascorbic acid were 1.548 ± 0.276, 2.077 ± 0.071 and 2.373 ± 0.055, respectively, which indicated that the reducing power of EAE had already reached 87.5% of that of ascorbic acid. This result indicated that the EAE of T. jasminoides possessed higher reducing power in comparison with the extracts from other plants that have been reported in recent years such as Momordica charantia, Gynura procumbens and so on [34,35].…”

Section: Reducing Powermentioning

confidence: 75%

Cytotoxic activities against MCF-7 and MDA-MB-231, antioxidant andα-glucosidase inhibitory activities ofTrachelospermum jasminoidesextractsin vitro

Song

Zhang

Gong

et al. 2019

Biotechnology & Biotechnological Equipment

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The present study investigated the cytotoxic activities against MCF-7 and MDA-MB-231 cells, and the potential antioxidant and a-glucosidase inhibitory activities of the extracts of the aerial parts of Trachelospermum jasminoides, a traditional medicinal plant used for the treatment of rheumatic arthralgia in China. In MTT assay and microscopic analysis, the ethyl acetate extract (EAE) of T. jasminoides showed moderate cytotoxic effect against MCF-7 and MDA-MB-231 with IC 50 values of 109.54 ± 2.39 lg/mL and 162.68 ± 9.52 lg/mL, respectively, but the water extract (WE) was inactive against both of them. With gallic acid and rutine as equivalents for determination, the total phenolic and total flavonoid contents in the EAE (54.93 ± 0.32 mg GAE/g and 186.75 ± 4.55 mg RE/ g, respectively) were higher than those of the WE. The antioxidant activities of the EAE and WE, which were evaluated by reducing power assay, DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging assay and hydroxyl radical scavenging assay, may be directly associated with the high contents of phenols and flavonoids in T. jasminoides. The EAE showed higher inhibitory effect in the a-glucosidase inhibition tests with an IC 50 value of 776.81 ± 107.80 lg/mL as compared to the WE with 2607.48 ± 364.31 lg/mL. Furthermore, the antioxidant activity and the inhibitory effects of the fractions against a-glucosidase were concentration-dependent. ARTICLE HISTORY

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Physicochemical properties and antioxidant activities of polysaccharides from Gynura procumbens leaves by fractional precipitation

Cited by 64 publications

References 23 publications

Characterization and Antioxidant Activities of Yellow Strain Flammulina velutipes (Jinhua Mushroom) Polysaccharides and Their Effects on ROS Content in L929 Cell

Characterization and Antioxidant Activities of Yellow Strain Flammulina velutipes (Jinhua Mushroom) Polysaccharides and Their Effects on ROS Content in L929 Cell

Physicochemical and Antioxidant Properties of the Degradations of Polysaccharides from Dendrobium officinale and Their Suitable Molecular Weight Range on Inducing HeLa Cell Apoptosis

Cytotoxic activities against MCF-7 and MDA-MB-231, antioxidant andα-glucosidase inhibitory activities ofTrachelospermum jasminoidesextractsin vitro

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