Characterization and Antioxidant Activities of Yellow Strain Flammulina velutipes (Jinhua Mushroom) Polysaccharides and Their Effects on ROS Content in L929 Cell

Hu, Yu‐Ning; Sung, Tzu-Jung; Chou, Chun-Hsu; Liu, Kai-Lun; Hsieh, Liang-Po; Hsieh, Chang‐Wei

doi:10.3390/antiox8080298

Cited by 51 publications

(20 citation statements)

References 46 publications

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“…This result is in agreement with a study showing the non-cytotoxicity of CS from shark cartilage (13). The inhibitory effect of the total intracellular ROS (DCFH-DA test) by CS-A tilapia was more efficient than other polysaccharides found in the literature, and similar effects were obtained with low concentrations of CS-A tilapia (13,18). These data are important to strengthen the hypothesis of the safe and efficient use of CS-A tilapia as an antioxidant and could be correlated during the treatment of oxidant stress diseases such as osteoarthritis and Alzheimer's disease (6,7,13).…”

Section: Discussionsupporting

confidence: 92%

Chondroitin sulfate from fish waste exhibits strong intracellular antioxidant potential

Medeiros

Vasconcelos

Silva

et al. 2021

Braz J Med Biol Res

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Chondroitin sulfate (CS) is a type of glycosaminoglycan described as an antioxidant molecule that has been found in animal species such as fish. Tilapia (Oreochromis niloticus) represents an eco-friendly source of this compound, since its economical processing generates usable waste, reducing the negative environmental impact. This waste was used for CS extraction, purification, characterization by enzymatic degradation, and evaluation of its antioxidant effect. CS obtained from tilapia presented sulfation mainly at carbon 4 of galactosamine, and it was not cytotoxic at concentrations up to 200 mg/mL. Furthermore, 100 mg/mL of CS from tilapia reduced the levels of reactive oxygen species to 47% of the total intracellular reactive oxygen species level. The ability of CS to chelate metal ions in vitro also suggested an ability to react with other pathways that generate oxidative radicals, such as the Haber-Weiss reaction, acting intracellularly in more than one way. Although the role of CS from tilapia remains unclear, the pharmacological effects described herein indicate that CS is a potential molecule for further study of the relationship between the structures and functions of chondroitin sulfates as antioxidants.

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Section: Discussionsupporting

confidence: 92%

Chondroitin sulfate from fish waste exhibits strong intracellular antioxidant potential

Medeiros

Vasconcelos

Silva

et al. 2021

Braz J Med Biol Res

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show abstract

“…The ABTS free radical scavenging activity was determined following the method modified by the authors [18], and the absorbance of the final reaction mixture was read at 734 nm against an appropriate blank. The ABTS RSA of the methanolic extract was calculated by the following equation: % RSA = [1 − Asample/Acontrol] × 100.…”

Section: Determination Of Antioxidant Capacitymentioning

confidence: 99%

Metabolic Profiles, Bioactive Compounds, and Antioxidant Capacity in Lentinula edodes Cultivated on Log versus Sawdust Substrates

2021

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Lentinula edodes (shiitake) is a popular nutritious edible mushroom with a desirable aroma and flavor. Traditional cultivation of L. edodes on beds of logs has been replaced by cultivation on sawdust, but the effects of cultivation changes on L. edodes mushrooms have not been well characterized. We determined the metabolic profile, bioactive compounds, and antioxidant capacity in L. edodes grown on log or sawdust substrates. Metabolic profiles of L. edodes extracts were determined by 1H nuclear magnetic resonance (NMR) and ultra-performance liquid chromatography to quadrupole time-of-flight mass spectrometry. Principal component analysis score plots from 1H NMR analysis showed clear differences between samples. Concentrations of primary metabolites, especially amino acids, generally decreased in L. edodes grown on logs compared to sawdust. Phenolic compounds showed variations in concentration depending on the cultivation method. Bioactive compounds and their antioxidant capacity were analyzed spectrophotometrically. L. edodes cultivated on logs had high concentrations of bioactive compounds with strong antioxidant capacity compared to L. edodes cultivated on sawdust. Thus, the concentration of primary metabolites was high in L. edodes grown on sawdust, which produces a high growth rate. In contrast, log-cultivated L. edodes, which were similar to wild mushrooms, had high levels of bioactive compounds and high antioxidant capacity. This information is useful for determining optimal cultivation conditions for nutritional and medicinal uses of L. edodes mushrooms.

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“…The ROS generation assay was performed using a previously described method, with modification [28]. Specifically, the concentration of ROS was evaluated using a DCF-DA probe (Sigma Aldrich).…”

Section: Ros Generationmentioning

confidence: 99%

Pholiota nameko Polysaccharides Promotes Cell Proliferation and Migration and Reduces ROS Content in H2O2-Induced L929 Cells

et al. 2020

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Pholiota nameko, a type of edible and medicinal fungus, is currently grown extensively for food and traditional medicine in China and Japan. It possesses various biological activities, such as anti-inflammatory, anti-hyperlipidemia and antitumor activities. However, P. nameko has rarely been discussed in the field of dermatology; identifying its biological activities could be beneficial in development of a new natural ingredient used in wound care. To evaluate its in vitro wound healing activities, the present study assessed the antioxidant and anti-collagenase activities of P. nameko polysaccharides (PNPs) prepared through fractional precipitation (40%, 60% and 80% (v/v)); the assessments were conducted using reducing power, hydroxyl radical scavenging activity, dichloro-dihydro-fluorescein diacetate and collagenase activity assays. The ability of PNPs to facilitate L929 fibroblast cell proliferation and migration was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and scratch assays. The findings indicated that, among all fractions, PNP-80 showed the best antioxidant and anti-collagenase activity, as measured by their reducing power (IC50 of PNP-80 was 2.43 ± 0.17 mg/mL), the hydroxyl radical scavenging (IC50 of PNP-80 was 2.74 ± 0.11 mg/mL) and collagenase activity assay, and significantly reduced cellular ROS content, compared with that of H2O2-induced L929 cells. Moreover, PNP-80 significantly promoted L929 fibroblast proliferation and migration, compared with the control group. Overall, we suggested that PNP-80 could be a promising candidate for further evaluation of its potential application on wound healing.

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Characterization and Antioxidant Activities of Yellow Strain Flammulina velutipes (Jinhua Mushroom) Polysaccharides and Their Effects on ROS Content in L929 Cell

Cited by 51 publications

References 46 publications

Chondroitin sulfate from fish waste exhibits strong intracellular antioxidant potential

Chondroitin sulfate from fish waste exhibits strong intracellular antioxidant potential

Metabolic Profiles, Bioactive Compounds, and Antioxidant Capacity in Lentinula edodes Cultivated on Log versus Sawdust Substrates

Pholiota nameko Polysaccharides Promotes Cell Proliferation and Migration and Reduces ROS Content in H2O2-Induced L929 Cells

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