Physico‐chemical studies on a wide composition range of low‐moisture glucose–fructose mixtures: rates of crystallisation

Arvanitoyannis, Ioannis S.

doi:10.1111/j.1365-2621.2008.01740.x

Cited by 1 publication

(1 citation statement)

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“…It induces dehydration and osmotic shrinkage of the cells; therefore, lowering the risk of intracellular ice crystallization (PLACHINTA et al, 2004). Using fructose is not very common in cryopreservation assays, but when associated with sucrose it has the ability to delay the crystallization rate by reducing the time of ice crystals nucleation (ARVANITOYANNIS, 2009). In other words, fructose may be used as a cryoprotectant agent with impermeable effect on the cells, because it has presented lower toxicity at low concentrations.…”

Section: Discussionmentioning

confidence: 99%

Surubim-do-Paraíba oocytes viability after being exposed to different cryoprotectants

et al. 2018

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To know the non-toxic cryoprotectants to fish oocytes is of extreme importance for tests that aim to increase oocyte resistance to cold, thus allowing more advanced studies in cryopreservation. Therefore, commonly used cryoprotectants such as methanol, dimethyl sulfoxide, ethylene glycol, propylene glycol, sucrose and fructose were studied. Immature oocytes from the initial to vitelogenic (diameter <1.7 mm) and mature (diameter >1.8 mm) stages of Steindachneridion parahybae were evaluated. Four distinct experiments were performed, three using immature oocytes and one using oocytes at the mature stage. For each oocyte stage, the best maintenance solution to be used: Hank or 50% L15 and; viability after baths for 30min (room temperature) at cryoprotectant concentrations ranging from 0.25 to 4M were evaluated. Different tests were used to evaluate oocyte viability: in vitro maturation followed by observation of germinal vesicle breakdown (only for immature oocytes), Trypan Blue staining (all stages) and fertilization and hatching rates (mature stage only). Results showed that the toxic effect of cryoprotectants on oocytes generally increases with increasing concentrations. Sensitivity of oocytes to cryoprotectants increases according to the stage of development, with mature oocytes being more sensitive. Sucrose, fructose, methanol, propylene glycol and dimethyl sulfoxide can be used as cryoprotectants for S. parahybae oocytes.

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Section: Discussionmentioning

confidence: 99%